Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	820,914	IS5 (+) +4 bp	coding (122‑125/714 nt)	ybhM →	Bax1‑I family protein YbhM

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	273955 =	NA (NA)	28 (0.610)	27/478	0.6	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	820914 =	0 (0.000)					coding (122/714 nt)	ybhM	Bax1‑I family protein YbhM
*	?	NC_000913	= 275149	NA (NA)	35 (0.770)	33/476	0.4	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 820917	0 (0.000)					coding (125/714 nt)	ybhM	Bax1‑I family protein YbhM

CGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTT  >  NC_000913/273713‑274188                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          cGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCGAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGc                                                                                                                                                                                                                                   <  1:422814/251‑1 (MQ=255)  gTCAAAGGGACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCg                                                                                                                                                                                                                                  <  2:484405/251‑1 (MQ=255)        ggCACCAAAGCACAGGTGGAAGCAATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAg                                                                                                                                                                                                                            <  1:386314/251‑1 (MQ=255)          cACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCg                                                                                                                                                                                                                          <  1:177353/251‑1 (MQ=255)            ccAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCggg                                                                                                                                                                                                                        >  1:479574/1‑251 (MQ=255)                 gCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCACCGCTG‑ACAGCCCTGTTATGGAAGGTGCGAATAATCGGGGAAAt                                                                                                                                                                                                                   <  2:66779/251‑1 (MQ=255)                   acaGGTGGAAGCCAGCAGGGAGGAGTTTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAAttc                                                                                                                                                                                                                 <  2:220255/251‑1 (MQ=255)                    caGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGGACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGACTCAACGATCCCGCAG‑AGTAAAGCCAGAAACATCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGGATAAGAGGGGGAAttct                                                                                                                                                                                                                >  2:129029/1‑251 (MQ=255)                         ggAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTtctc                                                                                                                                                                                                            <  1:440966/250‑1 (MQ=255)                                           gtgtCGGGGTGTGCTCGAAGGCAGTCTGAAACTCATGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttca                                                                                                                                                                                         <  2:14554/251‑1 (MQ=255)                                                   gtgtGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttcttc                                                                                                                                                                                  >  1:383972/1‑250 (MQ=255)                                                                      gaaatgcaggccgacaaTGGATAAGACGAAAAACCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑GCAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCGGCATGTTGGAGCCGAttttttt                                                                                                                                                              <  2:71984/235‑2 (MQ=38)                                                                       aaaCTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtct                                                                                                                                                             >  1:323894/1‑251 (MQ=255)                                                                          cTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTccc                                                                                                                                                          >  1:256758/1‑251 (MQ=255)                                                                                  gAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGAGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGCAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGCGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCTTGTTTGAGCCGGTTTTTTCTCCCGTAAATGc                                                                                                                                                  >  1:440484/1‑251 (MQ=255)                                                                                              gACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCCGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCCCTGTTATGGAAGGGGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTCAATGCCTTGAATCAGcc                                                                                                                                      >  2:281306/1‑251 (MQ=255)                                                                                              gACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGc                                                                                                                                                                                                                                   >  2:90810/1‑158 (MQ=255)                                                                                              gACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGc                                                                                                                                                                                                                                   <  1:90810/158‑1 (MQ=255)                                                                                                     aTCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACCGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAttt                                                                                                                                 >  2:169030/1‑249 (MQ=255)                                                                                                      tCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCCACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCCCTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCCGCCTAttt                                                                                                                                 >  1:29666/1‑248 (MQ=255)                                                                                                        ccccAGGTTAATGACGGTTTTATCTTTCTGGGGCAAAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCGCAACGATCCCGCAGGAG‑AAAGCCAGAAACGTCGTCGAATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                                                                                                                            <  2:252485/251‑1 (MQ=25)                                                                                                         cccATGTTAATGACGGCTTTATCTTTCTGGGGCCCAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTTGTCTCAACGGTCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTT‑ACTGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGTCTGTCTCAGTCCTTTCATTTCTTCATGTTTGAGCCGATTTTTTCCCCCGTAAATTCCTTGAATCAGCCTATTTAGACCg                                                                                                                           <  2:38241/251‑1 (MQ=25)                                                                                                              gTTAATGACGGCTTTATCTTTCTGGGGGACAGGCTCATTCGCAAACGCAGTCGTTATGGTGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGATCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttct                                                                                                                      <  2:330612/251‑1 (MQ=255)                                                                                                                  aTGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc                                                                                                                  >  1:108323/1‑251 (MQ=255)                                                                                                                                        gCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGt                                                                                            <  1:281306/251‑1 (MQ=255)                                                                                                                                                tCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                                    >  1:325343/1‑251 (MQ=255)                                                                                                                                                 cATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt                                                                                     <  1:290519/249‑1 (MQ=255)                                                                                                                                                    tCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCGTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTgaga                                                                                 <  2:127968/250‑1 (MQ=255)                                                                                                                                                       cAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGGGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCCGTTTTTAGTGAGAtctc                                                                             >  2:207691/1‑251 (MQ=255)                                                                                                                                                         aaCGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑TCAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAtctctc                                                                           >  1:159617/1‑251 (MQ=255)                                                                                                                                                           cGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTccc                                                                         <  1:303636/251‑1 (MQ=255)                                                                                                                                                               gTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                                                                                                                              <  1:55994/162‑1 (MQ=255)                                                                                                                                                               gTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                                                                                                                              >  2:55994/1‑162 (MQ=255)                                                                                                                                                               gTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                                                                                                                                <  1:469966/192‑1 (MQ=255)                                                                                                                                                               gTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                                                                                                                                >  2:469966/1‑192 (MQ=255)                                                                                                                                                                         cGAGATGCGAGTGGTCTTAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCTTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCAtt                                                           <  2:32443/251‑1 (MQ=255)                                                                                                                                                                            gaTGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTgg                                                        >  2:344866/1‑251 (MQ=255)                                                                                                                                                                                cGAGAGGTCTCAAAGATCCCGCAGGAG‑AAAGCCCGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGACTCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCCGTTTTTAGTGAGATCTCTCCCACTGCCGTATCATTTGGTccc                                                    >  2:434405/1‑250 (MQ=255)                                                                                                                                                                                   gTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCACGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCAATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGAGGTATCATTTgggtcgccc                                                 >  2:401453/1‑244 (MQ=255)                                                                                                                                                                                           aaCGATCCCGCAGGAG‑AAACCCATAAACCTCGCCGCATCG‑TGTACAGCACTGTTATGGAAGGTGCGAATCATCGGGGAAATTCCTCTCGGCTGACTCATTCATTTCAGTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGTATCAGCCTATTTATACCGTGTCTTCGCCATTTAAGGCGTTATCCCCAGTTGTTAGTGAGATCCCGCCCACTGACCTATCATTTGGTCCGCCCGatccagg                                         <  2:132319/251‑7 (MQ=14)                                                                                                                                                                                               aTCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑AAAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTgg                                     >  1:148447/1‑251 (MQ=255)                                                                                                                                                                                                                      aCTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGcc                 >  1:464104/1‑249 (MQ=255)                                                                                                                                                                                                                             cgcATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGtt         >  1:212139/1‑250 (MQ=39)                                                                                                                                                                                                                                  cGCTT‑ACAGCCCTGTTATGGAAGGTGCTAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTTAATCAGCCTATTTAGACCGTTTCGTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTTGCCAGCGTTAATAACATCGCCATTTTGTTATCGtt   <  1:175606/251‑1 (MQ=11)                                                                                                                                                                                                                                  cGCTG‑TCAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTCGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGt    <  2:379278/250‑1 (MQ=21)                                                                                                                                                                                                                                   gCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGttt  <  1:344866/251‑1 (MQ=35)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          CGTCAAAGGCACCAAAGCACAGGTGGAAGCCATCAGGGAGGAGTGTCGGGGTGTGCTCGAAGGCAGTCTGAAACTCAGGCTGAACATGGATAAGACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAG‑AAAGCCAGAAACTTCGCCGCATCGCTG‑ACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTT  >  NC_000913/273713‑274188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.