Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,595,570	(A)6→5	coding (282/915 nt)	lgoR ←	putative DNA‑binding transcriptional regulator LgoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,595,570	0	A	.	100.0%	220.8 / NA	49	coding (282/915 nt)	lgoR	putative DNA‑binding transcriptional regulator LgoR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (25/24);  total (25/24)

GAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC  >  NC_000913/4595324‑4595819                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          gagaAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTg                                                                                                                                                                                                                                                      <  1:44391/251‑1 (MQ=255) gagaAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGAGCTGGTTTATCATCGTGAAAAA‑GCCTg                                                                                                                                                                                                                                                      >  2:325665/1‑251 (MQ=255)    aaaGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGACTTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTc                                                                                                                                                                                                                                                   >  1:132224/1‑251 (MQ=255)         gCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAaca                                                                                                                                                                                                                                              <  1:325665/251‑1 (MQ=255)            gAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACtt                                                                                                                                                                                                                                           >  1:74758/1‑251 (MQ=255)             aaGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACttt                                                                                                                                                                                                                                          >  2:391028/1‑251 (MQ=255)                            gAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCa                                                                                                                                                                                                                            >  2:323757/1‑250 (MQ=255)                            gAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCa                                                                                                                                                                                                                            <  1:323757/250‑1 (MQ=255)                                   tCGCGTAGCTCAAACAGCTGCTCGGCGTAGGAGTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTGTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATGCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGc                                                                                                                                                                                                                     <  1:317566/250‑1 (MQ=255)                                         aGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCTAACACTTTGTTTTGTTCACTCATTGAAGCGgtagt                                                                                                                                                                                                               >  2:216229/1‑250 (MQ=255)                                                               aGGATTGATCGAATTTCTTAATGCTCCACTGGCCGCGCTTTTCGCTGGGAAGAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACTCCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCGGCTCAAACACTTTGTGTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGt                                                                                                                                                                                        <  2:344007/251‑1 (MQ=255)                                                               aGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGt                                                                                                                                                                                        >  1:478970/1‑251 (MQ=255)                                                                 gATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCa                                                                                                                                                                                      <  1:342861/251‑1 (MQ=255)                                                                 gATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGACGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCa                                                                                                                                                                                      <  1:387784/251‑1 (MQ=255)                                                                  aTTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAg                                                                                                                                                                                     <  2:74758/251‑1 (MQ=255)                                                                                    tGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACAt                                                                                                                                                                   >  1:254670/1‑251 (MQ=255)                                                                                     gCTCCACTGGCCGCGCCTTTCGCTTTGATTAAGATTATAACGGCCGAATTTTAAAATATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGATCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACAt                                                                                                                                                                   <  1:56045/250‑1 (MQ=255)                                                                                                 gcgcTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAAc                                                                                                                                                      <  2:425588/251‑1 (MQ=255)                                                                                                         cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTAACATCGTGAAAAA‑GCATGCTCAAACCCTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGTCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGACGTTTCCAACCTgattc                                                                                                                                               >  2:418367/1‑246 (MQ=255)                                                                                                                    aGATTATCACGGCATAATGTTAAAAGATATTTGCTTAACAAAACCGGGGTGGCACCCTCGGGCAGAGCTAGTTGCAGTGCAGAAAACGTTTCCCCTGGGCGTAAGTGGCGCTGGTTTATCATCGTGAAAAA‑GCGTGCTCAAACACTTTGTTTTGTTCACTCATTGAATCTGTAGTACAGTCGAACCCGTCATCATGGTCAGGTTTGCGCGCAAGAACATAGTCGTTGCCATCCTGCTTCAAATCGCCGCAt                                                                                                                                   <  2:337011/251‑1 (MQ=255)                                                                                                                      aTTATAACGGCCGCATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGCCGCCGCATTc                                                                                                                                 >  2:220503/1‑251 (MQ=255)                                                                                                                      aTTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTc                                                                                                                                 <  1:65126/251‑1 (MQ=255)                                                                                                                          taACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGt                                                                                                                             >  1:118375/1‑251 (MQ=255)                                                                                                                              ggCCGAATTTTAAAAGATATTCTCTTACTACAAACGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGTGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCACAAACACTTTGTTTTGTTCACTCATTGCAGCGGTAGTACAGTCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCAGCGTCAAGACGCCGCATTCGCGTAAgg                                                                                                                         >  2:260736/1‑250 (MQ=255)                                                                                                                                    aTTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTg                                                                                                                    >  1:379612/1‑250 (MQ=255)                                                                                                                                        tAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCCAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAGGCGCAATACCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAttagatcggaagcacgtcgtg                                                                                                               >  2:473417/1‑232 (MQ=255)                                                                                                                                        tAAAAGATATTCTCGTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                                                                  <  1:473417/232‑1 (MQ=255)                                                                                                                                            aGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                                                                           <  1:445727/251‑1 (MQ=255)                                                                                                                                            aGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                                                                           <  1:391028/251‑1 (MQ=255)                                                                                                                                                    tctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGGGAATATGACGCACtgt                                                                                                   >  2:141901/1‑251 (MQ=255)                                                                                                                                                     ctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgtg                                                                                                  >  1:121423/1‑251 (MQ=255)                                                                                                                                                         ctacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggggc                                                                                              >  1:259164/1‑248 (MQ=255)                                                                                                                                                            cAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTg                                                                                                                                                   <  2:443570/195‑1 (MQ=255)                                                                                                                                                            cAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTg                                                                                                                                                   >  1:443570/1‑195 (MQ=255)                                                                                                                                                             aaCCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCt                                                                                          >  1:21154/1‑251 (MQ=255)                                                                                                                                                                cGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAt                                                                                       >  1:485546/1‑251 (MQ=255)                                                                                                                                                                 gggCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtg                                                                                      >  1:245775/1‑251 (MQ=255)                                                                                                                                                                     tGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCtgttgttgt                                                                                  >  1:19563/1‑251 (MQ=255)                                                                                                                                                                                       ccAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGGTGTTGTACCTTTCCGCCAgcgccc                                                                >  2:460309/1‑249 (MQ=255)                                                                                                                                                                                            tGCAGTTCAGAAAACGTTTCCCCTGTGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTg                                                            <  1:231276/250‑1 (MQ=255)                                                                                                                                                                                             gCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGGTTATCATCGTGAAAAA‑GCCGGCTCAAACACTTTGTTTTGTTCACTCATTGAAGAGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGCTGTTGTACATTTCCGCCAGCGCACTTTGc                                                           <  2:347257/250‑1 (MQ=255)                                                                                                                                                                                                    aGAAAACGTTGCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTGGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGGCAGGTTTACGCGCAATAACATAGTCGTTGCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTGCATTTCCGCCAGCGCACGTTGCGATGGAAg                                                   <  1:220503/251‑1 (MQ=255)                                                                                                                                                                                                         aCGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTgggg                                              >  1:475683/1‑251 (MQ=255)                                                                                                                                                                                                          cGTTTCCCCTGGGCGTAACTGGCGCTGGTTGATCATCGTGAAAAT‑GCCTGCTCAAACACTTTGTGTTGTTCACTCATTGAAGCGGTAGGACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAAGAACATAGTCGGTTCCAACCGGCGTCAAGACGCCGCATTCTCGTAAGTGGCTGAGAATATGACGCGCGGTGGTGCGGCTGATGGTGTACATTTCCTCCAGCGCACTTTGCGATGGAAGTGGGGa                                             <  2:53797/251‑1 (MQ=255)                                                                                                                                                                                                               ccccTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAggg                                         <  1:220074/250‑1 (MQ=255)                                                                                                                                                                                                                        gTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGAcccc                               <  1:324041/251‑1 (MQ=255)                                                                                                                                                                                                                        gTAACTGGCGCTGGTTTATCATCGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGAcccc                               <  1:476008/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggattATCATCGAGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGGGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACACCGCGCCATatcat                   >  2:105031/4‑251 (MQ=255)                                                                                                                                                                                                                                           aatcGTGAAAAA‑GCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGGCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGAc            <  2:29870/250‑1 (MQ=255)                                                                                                                                                                                                                                                  gaaaaaGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAc                                                                                                          <  1:415258/150‑1 (MQ=255)                                                                                                                                                                                                                                                  gaaaaaGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAc                                                                                                          >  2:415258/2‑151 (MQ=255)                                                                                                                                                                                                                                                       aGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCCGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCCTATCATCAATGACCTGGTTAATc  >  1:446512/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          GAGAAAGTGCTGAAGTGAATGTGTTTCGAGCATTTCGCGTAGCTCAAACAGCTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATC  >  NC_000913/4595324‑4595819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑