Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,549	Δ1 bp	coding (179/606 nt)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,549	0	G	.	100.0%	225.0 / NA	50	coding (179/606 nt)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (29/21);  total (29/21)

GTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCA  >  NC_000913/3207304‑3207795                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gtcgCGGCACGCTGCTGGTTCATGCCGATCTCTCCTTTGAAAATCTGTCTCACCCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTTAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCAt                                                                                                                                                                                                                                                  <  1:423099/251‑1 (MQ=255)      ggCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAACTCTGTCTCACACCCGGAGCGCGTTATGAAACAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCCAGATATTCGCGTCGGCGATGTGATCTAACTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGACACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTgc                                                                                                                                                                                                                                             >  2:232981/1‑251 (MQ=255)                       tGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGCTCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGTTCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAACAACGGCGACaa                                                                                                                                                                                                                             >  2:419745/1‑250 (MQ=255)                         ccGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATg                                                                                                                                                                                                                          >  1:39554/1‑251 (MQ=255)                               ccccctttaaaaaccgtctccacccccGGAGCGCGTTATAAAAAAGACCCTAACAACCCCGAACAAAGAGAAATATCGGCAAGTTATTCGGGTCGGCGATGGAATCGCCCTGACCGTTGCTCTGTGGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATg                                                                                                                                                                                                                          <  2:195975/221‑1 (MQ=255)                                 cccTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGCCGTTTGTCACCGCCGTTTGCTCGAACTGAAACGTCCGATCCCTTACGATCTCCACGGCAAAGAGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGTGATgg                                                                                                                                                                                                                  >  2:325630/2‑251 (MQ=255)                                     ttGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCACAGCGCTTTTCCACGCTG‑CCCCCTCGTGCGCAAAAACGGCGACAAATGGGGGATGGtctc                                                                                                                                                                                                              >  2:55385/1‑251 (MQ=255)                                       aaaaaTCTGCCTCACCCCCGGAGCGCTTTAGGAAAAGGATCCTGACAACCCCGATAAAAGCGGAAGATCTGCAAGATATCGGCGTCGGCGAGGTGATCTCCCTGCCCGGTACGCTGGTGACCGGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCg                                                                                                                                                                                                            <  2:183532/250‑1 (MQ=255)                                               gtcccacaCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGcc                                                                                                                                                                                                      <  1:349237/245‑1 (MQ=255)                                               gcctcccccccggaggcggTTATAAAAAAGACCCTGACAACCCCGAACAATGGTGAAGATTTGAAAGATATTCGGGGCGGGGATGTTATCTCCCGGCCCGGAAGGCTGGTGACCTGCCGCGACGTTGGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCg                                                                                                                                                                                                    <  2:41234/233‑1 (MQ=255)                                                  ccacaCCCGGAGCGCGTTATGAAAAAGATCCTGACACCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGAGGTGATCTACCTGACCGGCACGCGGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACa                                                                                                                                                                                                 <  1:55385/250‑1 (MQ=255)                                                     cacCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGCTGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCCACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAcc                                                                                                                                                                                              >  1:292164/1‑251 (MQ=255)                                                       cccGGGGCGCGTGATGAAAAAGATCCGGACAACCCCCATCAAAGCTGAAGATGTGCAAGATATTCGCTCCGGCGATGTAATTTCCCTTACTGCTACGCTGGTGACCTCCCGCGACGTTTTTCCCCGCCGTTTGATCGAACTGAAACGTCCGAGCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAg                                                                                                                                                                                            <  1:450716/251‑1 (MQ=255)                                                          ggAGCGCGTTAGGAAAAAGATCCTGACAACCCCGATAAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAt                                                                                                                                                                                         <  1:404390/251‑1 (MQ=255)                                                           gAGCGGGTTATGAAAAAGATCCTGAAAACCCCGATCAAATCTGAAGATCTGCAAGATATTCGCTACGGCGATGTGATTTCCCTGACCGGTACGCTGGTGACCTTCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATg                                                                                                                                                                                        <  1:143356/251‑1 (MQ=255)                                                             gcgcGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCCAAAACGGCGAAAAATGGGAGATGGTCTCCGACGGCCCGCCAACCAGTATggg                                                                                                                                                                                      >  2:444044/1‑249 (MQ=255)                                                                     tGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGaa                                                                                                                                                                               >  1:236756/1‑250 (MQ=255)                                                                            aaTCCTGACATCCCCGATCAAAGCGGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTCCCGGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGa                                                                                                                                                                       <  1:418864/250‑1 (MQ=255)                                                                                  gACAACCCCGATCAACGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACATGACCGGTACGCTGGTGACCTGCCGCGCCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAACGCGATTTTCCACGCTG‑CCCCATCGTGCGAAAAAACGGCGACAAATAGGAGATGGTCTCCGTCGGCCCGACAAACAGTATGCGTATGGAAAGTTTAGAACg                                                                                                                                                                    >  2:449288/1‑248 (MQ=255)                                                                                     aaCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGCTGGTCTCCGTCGGCCCGACAACCAGTATGCGTATg                                                                                                                                                                                  >  2:92078/1‑231 (MQ=255)                                                                                     aaCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAAtt                                                                                                                                                              >  2:320270/1‑251 (MQ=255)                                                                                     aaCCCCGATCAAAGCTGAAGATCGGCAAGATATTCGCGTGGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATg                                                                                                                                                                                  <  1:92078/231‑1 (MQ=255)                                                                                      cccccGATCAAAGCTGAACACCGGCAAGAGAGTCGCGTCGGCGATTTGTGCTACCGTACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTGCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAAttt                                                                                                                                                             <  1:53932/250‑1 (MQ=255)                                                                                       ccccGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGAATGGAAAGATTTGAACGTGAAttt                                                                                                                                                             >  2:168043/1‑250 (MQ=255)                                                                                              cAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGc                                                                                                                                                      >  1:126785/1‑250 (MQ=255)                                                                                                 agctgataagcttcAAGATATTCTCATCGGCAAGGTAATCTACTGGTCCGGTGCGCTGGTGACCTGCCGGTAATTTTGTCACCGCCTTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGCTGGTCTCCGTCGGTCCGACAACCAGTATGCGTATGGAAAGTGTTGAACGTGAATTTATTGAGCAGAc                                                                                                                                                  <  1:347937/238‑1 (MQ=255)                                                                                                       aGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCggagt                                                                                                                                            >  1:16676/1‑248 (MQ=255)                                                                                                         aTCTGCATGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTACCGCGCCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAACGCGATTTTCCACGCTG‑CCCCATCGTGCGCCAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTCTGGAAAGTTTTGAACGTGAGTTTATTGAGCCGACCGGCGTg                                                                                                                                           >  2:423049/1‑250 (MQ=255)                                                                                                                cctacccggcgtcggggtggtgttCTACCTGCCCGGTACGCTGGTGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtggt                                                                                                                                   <  2:419877/228‑1 (MQ=255)                                                                                                                       cgcgTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtggt                                                                                                                                   >  1:419877/1‑244 (MQ=255)                                                                                                                          gTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTCCGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAACCTGGTGGTTGGCaa                                                                                                                          >  1:308329/1‑250 (MQ=255)                                                                                                                                    tgATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGCTTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAACGGTGGTATgg                                                                                                               >  1:219293/1‑251 (MQ=255)                                                                                                                                                  ggTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCCACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCTAATGTGGTATGGGGCCGCTGGCCg                                                                                                   >  2:122705/1‑249 (MQ=255)                                                                                                                                                     ccgcttgtaaCCTGCCTCGACTTTTGTACCCGCCGTTTGATCGAACGGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCgaagt                                                                                               <  2:16676/241‑2 (MQ=255)                                                                                                                                                             gACCTGCCGCGACGTTTGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGcc                                                                                       <  1:320270/250‑1 (MQ=255)                                                                                                                                                                               tCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGCCAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTAc                                                                     >  1:370956/1‑250 (MQ=255)                                                                                                                                                                                    gccgTTTGATCGAACTGAAACGGCCGATCCCTTACGATCTCAACGGCAAAGCGATTGTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGa                                                               <  1:145709/251‑1 (MQ=255)                                                                                                                                                                                                   ggaaagttCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTAGTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGTCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAGTTTCCCGGCAGGCt                                                <  2:385837/244‑1 (MQ=255)                                                                                                                                                                                                             gATCCCTTACGATCTCATCGGCGAAGCGAGTTTCCACGCTG‑CCCCATCGTGCGAAAAAACGGCGACAAATGGGGGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTTACCGAAGAAGGCTGCCAGAACTTCAAGGCGCTACATGTGAGTTTCCCGGCAGGCTGCTCGGTGCt                                      <  2:227756/251‑1 (MQ=255)                                                                                                                                                                                                             aaTCCCTTACGATCTCAACGGCAAAGCGATTTTACACGCTG‑CCCCATCTTGCGCAAAACCGGCGACAAATAGGAGATGGTCTCCGTCGGCCCGAAAACCCGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGGGGTATGGGGCCGCTGACCGCAGAAGGCTGCCAGAAATACAAAGCGCTACAAGTGATTTTCCCGGCCGGCTGCGCGGTGCt                                      >  2:389315/2‑251 (MQ=255)                                                                                                                                                                                                                cccTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTggg                                   >  2:371843/1‑250 (MQ=255)                                                                                                                                                                                                                   ttACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTggcggc                                >  1:411190/1‑251 (MQ=255)                                                                                                                                                                                                                    tACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTggcggc                                >  1:48835/1‑250 (MQ=255)                                                                                                                                                                                                                      cGATCTCAACGGCAAAACGATTTTCCCAGCTG‑CCCCATCGGGCGCAAAAACGGCGACAAATGGGAGATGGTCTCAGTCGGCACGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGAACGGCGTGAAACTGGTGGTTGGCAAAGGTGGAATGGGGCCGCTGACAGAAGAAGGCTGCAAGAAATTCAAGGCGCAACATGTGATTTTTCGGGCCGGATGCGGAGTGCTGGAGGCAAc                             >  1:264662/1‑251 (MQ=255)                                                                                                                                                                                                                          ctcAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAccccg                         >  1:404093/1‑249 (MQ=255)                                                                                                                                                                                                                            cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAcc                            <  1:325630/246‑1 (MQ=255)                                                                                                                                                                                                                             aaCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGAGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGGGGTTGGCAAAGGGGGTATGGGGCCGCTGACCGAAGAAGGCGGCCAGAAATTCAAGGCGCTACAGGTGATTTTCCCGGGAGGCTgggagggggcggcggacaaccagggg                      >  2:321410/1‑226 (MQ=255)                                                                                                                                                                                                                                ggCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGGGGTATGGGGCCGCTGACCGGAGAAGGATGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGGGCTGGCGGCAAcccggggggcg                   >  2:129928/1‑243 (MQ=255)                                                                                                                                                                                                                                 gCAAAGCGATTTTCCACGCTG‑ACCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAATAATGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAag                  <  1:209726/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaaGCGATTTTCCACGCTG‑CCCCCTCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGACAGATTTGAACGGGCATTAATTGCGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGAGGGATGGTGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGTCGCTTCATGTGATTTTCCCGGCAGGCTGCGCGGTGATGACGGCACCCCAggaggacg                  >  2:336276/1‑247 (MQ=255)                                                                                                                                                                                                                                                  cgcggCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGGGCTGGCGGCAACCCATGTGGAAGAGATTGAAGAAGTGCa  <  2:446259/248‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCA  >  NC_000913/3207304‑3207795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑