Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,275,902	A→T	E207D (GAA→GAT)	garD →	GarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,275,902	0	A	T	97.7%	135.5 / ‑3.3	44	E207D (GAA→GAT)	garD	GarD
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (15/28);  total (15/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01

GCCGATGGCAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATG  >  NC_000913/3275657‑3276139                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               gCCGATGGCAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAAt                                                                                                                                                                                                                                           <  1:118485/251‑1 (MQ=255)            gggggccACCAAAACCCTGCTCGGTAGCACCACCAAGGTCCACTGTGTGGCAGGCGTGGTTGATTATGTAGTAAAAATCATTGAACGCGATCTGCTACTGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGcc                                                                                                                                                                                                                                <  2:194726/245‑1 (MQ=255)            cGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGcc                                                                                                                                                                                                                                <  1:453421/251‑1 (MQ=255)                  cACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGtt                                                                                                                                                                                                                          <  1:329827/251‑1 (MQ=255)                           ccTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGGTTACAGCACCCGCGGCAGGTGTACCCATTCGTACCATTCAAAAAATTTCGCTG‑GATCCTTACTTTGGCGGGGGTGTAATGGTGGTTGGCCTGGGtttggggaagg                                                                                                                                                                                                                 >  1:32620/1‑242 (MQ=255)                           ccTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGGTTAAAGCACCGGCGGCAGTTGTAACTATCCGTTCCATTCACAATATTTCGCTA‑AATCCTAAATTTTGCGGCGGTGGAATTGGGATTGGCCTGGGGTGGGaaaaat                                                                                                                                                                                                                 >  1:340961/1‑249 (MQ=255)                                  ggTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTAACTATCCGTACCATTCCAAATATTTCGCTG‑AATCCTAACTTTTGCGGCGATGTAATGGGGATTGGCCTGGGTTTGGAAAAGTTgccgcc                                                                                                                                                                                                          >  1:469270/1‑251 (MQ=255)                                                   ccACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGGGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTg                                                                                                                                                                                         <  2:340961/251‑1 (MQ=255)                                                                        ggACTATGTAGTAAAAAGCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCGTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTa                                                                                                                                                                   <  1:26012/251‑1 (MQ=255)                                                                          actttGTAGTAAAAATCATGGAACGCGATCTGCTACAGAAATGCCCGAACTTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTAtt                                                                                                                                                                 <  2:454098/247‑1 (MQ=255)                                                                             aTGTAGTAAAGATCATGGAACGCGATGTGTTCCCTAAATAGCCGAACTTCGATGGCGTGGGGGGGCTGCAGCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTAACTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACCGATGATGTG‑CAAGTTATTCCa                                                                                                                                                              <  2:445766/251‑1 (MQ=255)                                                                             aTGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCa                                                                                                                                                              <  1:285596/251‑1 (MQ=255)                                                                                     aaaaTCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAAAGCACCGGCGGCAGTTGTAACTATCCGTACCATTCACAATATTTTGCTG‑AATCCTCACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGGTGTGGAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGGTGATGTG‑CAAACCATTCCAGTAGaa                                                                                                                                                        >  1:308791/1‑249 (MQ=255)                                                                                          cATTGAACGCGATCTTCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCa                                                                                                                                                 <  2:469270/251‑1 (MQ=255)                                                                                           aTTGAATGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAg                                                                                                                                                <  2:113304/251‑1 (MQ=255)                                                                                              gAACGCGACCTGCTCCCGAAATCCCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTGGTGGCGTTGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATAGTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCAt                                                                                                                                             <  2:246970/251‑1 (MQ=255)                                                                                                      tCTGTTACCGAAATACCCGAACGTGGATGGCGTGGTGGGGCTGAATCATGTGTACGTTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGt                                                                                                                                      <  2:367973/250‑1 (MQ=255)                                                                                                         gCTACCGAAATACCCGAACGTCGATGGCCTGGTGGGGCTGAAGCATTTGTACGGTGGTGGCGTGGCGAATAACGCTCCGGCGGCAGTTGTATCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTGGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGAGGATGTG‑CAAGCTATTCCAGTAGAAAGCTCCATCATTGTCa                                                                                                                                        <  2:357882/245‑1 (MQ=255)                                                                                                          cTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCCGCGGCAGTTGTACCTATCCGTACCCTTCCCAAAAATTCGCTG‑GATCCCACATTTGGCGGCGATGTAATTGTGGTTGGTCTGGGGTGGGGAAAATTGCCACCTGGGCGCCCGCTGGATGGAACGGAAG‑GGTGGTAAGCTATTTCAGTAGAAAACGccctccttgtcaggtatt                                                                                                                                  >  2:419790/1‑234 (MQ=255)                                                                                                            aCCGAAAAACCCGAACGTCGATGGCGTGGGGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAgg                                                                                                                               <  2:432358/251‑1 (MQ=255)                                                                                                             ccGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTAGCCGTACCACTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCTCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGa                                                                                                                              <  1:189731/251‑1 (MQ=255)                                                                                                                  attcccGAACGTCGATGGCGTGGTGGGGCTGAAGCATTTGTTCGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGGAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGaaa                                                                                                                         <  2:32620/248‑1 (MQ=255)                                                                                                                   tACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTTGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGGGGTTTGCCTGGGGTGTGAAAAAGTGAAGCCTGAGCGCCTGCTGACTGGAACGGATGGTGTG‑CAAACTATTTCAGTAGAAAGCGCCAGCATTGttcggttgctaggtgaaa                                                                                                                         >  1:406994/1‑233 (MQ=255)                                                                                                                        gAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTTGCTG‑AATCCCAACTTTGGCGGCGGTGTAATGGGGGTTGGCCTGGGGTGTGAAAAGATGCAGCCTTCGCGCCTGCTGACTGGGACGGATGATGTG‑CAAGCCATTCCAGTAAAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATg                                                                                                                   >  1:236335/1‑251 (MQ=255)                                                                                                                               gATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGGGGCGTGGCGATTAACGCGCCGGAGGCAGTTGTAACTATCCGCCCCATTACCAATATTTCGCTG‑AATCCTATCGTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTTTTAGTGTGCAGGATGAAAAGCATGTCGGttt                                                                                                            <  1:360272/251‑1 (MQ=255)                                                                                                                                  ggCGTGGGGGGGCTGAATCATTTGTACGGGTGGGGCGTGGCGAATAACGCACCGGCGGCAGTTGTACCTATTCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCa                                                                                                         <  2:366173/251‑1 (MQ=255)                                                                                                                                                                     cgtggcgATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAAc                                                                      <  1:21016/251‑1 (MQ=255)                                                                                                                                                                           gATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTCGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCa                                                                                                         >  1:59787/1‑210 (MQ=255)                                                                                                                                                                                aCGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACa                                                            >  1:361894/1‑250 (MQ=255)                                                                                                                                                                                      cggcggCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGCTTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGGTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAACACTg                                                     >  2:100496/1‑251 (MQ=255)                                                                                                                                                                                            cAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATcc                                                >  2:193679/1‑249 (MQ=255)                                                                                                                                                                                              gTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACg                                             <  2:149643/251‑1 (MQ=255)                                                                                                                                                                                                            gaCCATTCACAATATTGCGCTG‑AATCCTAACTGTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCt                               <  2:212653/250‑1 (MQ=255)                                                                                                                                                                                                               cATTTACAATATTTCG‑TGTAACCCTATCTTTGGCTGCGATGTAATGGTGATTGGCCTTGGTTGTGAAAAGTCGTAGCCTGGTCGCCTGCTGACTGGAACGTATGATGTG‑CCAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAGCCTGcc                            <  2:419055/251‑1 (MQ=255)                                                                                                                                                                                                               cATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAGTCAACGGCAGCGAGAAACCGGcc                            >  2:430137/1‑251 (MQ=255)                                                                                                                                                                                                                attctcaatctgtcacTG‑AATCCTATCTTTGGCGGCGATGGAATGGTGATTGGCCGTGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGAGGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGGCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAATCCTGcca                           <  2:55301/236‑2 (MQ=255)                                                                                                                                                                                                                         atTTCGCTG‑AATCCTCACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAACAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTc                     >  2:348318/1‑248 (MQ=255)                                                                                                                                                                                                                             cGCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTg              <  2:236335/251‑1 (MQ=255)                                                                                                                                                                                                                              gCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTgg             <  1:282011/251‑1 (MQ=255)                                                                                                                                                                                                                              gCTG‑AATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTgg             <  1:193679/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaTCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGt         <  1:449120/251‑1 (MQ=255)                                                                                                                                                                                                                                    aTCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGCGAAACCTGCCCGGCTTCAGACATGGTCGtt        >  2:201520/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaCTTTTGCGGCGATGTAATGGGGACTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGGTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGAGCGCCGAACGCCATCTACAAAATCTGAATCAACGGAAGCGAGAAACTTGCCCGGCTTCAGAACTGGTCGTTGGt     <  2:327816/249‑1 (MQ=255)                                                                                                                                                                                                                                          aCTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATg  >  1:216350/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               GCCGATGGCAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG‑AATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTG‑CAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATG  >  NC_000913/3275657‑3276139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑