Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,497,3850TC94.7% 50.2 / ‑4.1 19F177S (TTT→TCT) opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (4/14);  total (5/14)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01
*NC_0009131,497,3860T.94.7% 65.5 / ‑1.2 19coding (531/1656 nt)opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (4/14);  total (5/14)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01
*NC_0009131,497,3870CA94.7% 54.9 / NA 19L178N (CTC→AAC) opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (4/14);  total (5/14)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
*NC_0009131,497,3880TA94.7% 51.1 / ‑3.8 19L178N (CTC→AAC) opgDglucans biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (4/14);  total (5/14)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

ACTGGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGG  >  NC_000913/1497155‑1497618
                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                       
aatGGACGCTCAGTTCTTCCATATGGGACTGGGAGTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCcg                                                                                                                                                                                                                      <  2:49683/249‑1 (MQ=255)
                    atatGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATc                                                                                                                                                                                                  <  1:131691/251‑1 (MQ=255)
                                    ccGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGc                                                                                                                                                                                  <  1:214679/251‑1 (MQ=255)
                                         cccgcTTTCGTTTGTTTTCTGGTGATCCAGCAGCTCATCGGGGGCGTTAATTTCCCTTGCGCCCGGAGTTGTTCAAATACAACGATGCAGGTTTTGATACAAAACAAGTAGAAGGGAAAAGCGATCTCGGCTTTGCCGGTTTTCGCCTGTTTAAAGCCCCCGAACTGGCGCGCCGTTATGTAGTATCATCAACGGCGCGTGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCcgcg                                                                                                                                                                             <  2:8838/250‑1 (MQ=255)
                                                 cGTATGTTTTCTGTTGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGATGTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAATTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGc                                                                                                                                                                      <  2:213650/250‑1 (MQ=255)
                                                        tttCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCGGCGCCGTGGATGATACATATAAATACGGTTGGTCGGccgccggccggggggccgcc                                                                                                                                                               >  1:120272/1‑232 (MQ=255)
                                                                   ccAGCAACACATCTGGCGCGGTAAATTCACTTTCGTCCGGAGGTGTTCAAATACAACGATTCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGAc                                                                                                                                                   <  2:31332/251‑1 (MQ=255)
                                                                                     cgTGAAATTCACTTTCGCCCGGAGTTGTTCAAATAAAACGATGCAGGGTTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTcccc                                                                                                                                 <  2:218021/251‑1 (MQ=255)
                                                                                                        gggAGTTGTTCAAATACAACGATGCAGGTGTTGATCCAAAACAATTAGAAGGGCAATGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTGAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATCTCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACTCTTACACCGACAGTAAAGAAGAGTTCCCCGTCTTTACCGCCTTCTGGt                                                                                                              <  1:11040/250‑1 (MQ=255)
                                                                                                              tgttCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCAATCGACACTTACACCGCAAGTAAAGAAGAGTTCCCCGACTTTACCGcccttgggtttga                                                                                                          >  2:164618/1‑239 (MQ=255)
                                                                                                                  cAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACgg                                                                                                     <  1:91890/250‑1 (MQ=255)
                                                                                                                  cAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGt                                                                                                    <  1:105198/251‑1 (MQ=255)
                                                                                                                    aaTACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTaa                                                                                                  <  1:293868/251‑1 (MQ=255)
                                                                                                                                cAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCAGCAACGGCGCGAGTTATTTCCGCGTCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTAACACCGACATTAAAGAAGAGTTCCACGACTTTACAGCCTTCTGGTTTGATACGGTAAAACCGGGGGCa                                                                                       >  1:227351/1‑250 (MQ=255)
                                                                                                                                           aCAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGTTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTAc                                                                            <  1:284539/250‑1 (MQ=255)
                                                                                                                                                     tAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTGCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCg                                                                  <  1:130209/250‑1 (MQ=255)
                                                                                                                                                          aggCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTATGCGCCGTTGATGATACATATCAATACGGTTTGGCGTCCCGCGGCCTGGCGTTCGAAACATACACAGACAGTTACGAAGAGTTCCCCGTCTTTACCGCCATCTGGCTTGATACGGTAAAACCGTGGGCAACTACCGTTACCGTTTAGTCGTTGCTc                                                            >  2:61356/2‑251 (MQ=255)
                                                                                                                                                                               gCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTAtagatcggaagagcacacgtctg                                               <  1:134851/243‑23 (MQ=255)
                                                                                                                                                                                                                    gcCGTGATGTAGTATCATCAACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAgg  >  1:137369/1‑251 (MQ=255)
                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                       
ACTGGACGCTCAGTTCTTCCATATGGGAATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTGTGAGAAAAGTCAGG  >  NC_000913/1497155‑1497618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: