Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,770,5390CT81.0% 36.1 / 5.3 21V281M (GTG→ATG) yibHinner membrane protein YibH
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base T (5/12);  total (9/12)
Fisher's exact test for biased strand distribution p-value = 2.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.60e-01

GAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGC  >  NC_000913/3770307‑3770777
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              
gaatcATGGCTGAAATGGTCGGAGTAAACCGCCCCCTGGGCGTAGATGCCGTCGGTTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCTTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGcc                                                                                                                                                                                                                              <  1:27160/247‑1 (MQ=255)
gAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACCCCACTTCCGCATCATCGcc                                                                                                                                                                                                                              >  1:30052/1‑251 (MQ=255)
            aaaTGGTCGTAGTAAACCGCCACCTGGTGGTAGAGGCCGGCGTGTAAGGCAACGATATCAGCGTTAGGGTCCAGTTAAATGGTTCCCAGCACACCGTCCGGTCCGGGCACGACCGTTAATGATTGCAATCCCCCCTGCGCCAGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACCCCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGAc                                                                                                                                                                                                                   <  2:48500/250‑1 (MQ=255)
                                     gggCGTAGATGCCGGCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCTCTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTgg                                                                                                                                                                                         <  1:175149/251‑1 (MQ=255)
                                               gCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAAttt                                                                                                                                                                               <  1:194534/251‑1 (MQ=255)
                                                   tCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCg                                                                                                                                                                           >  1:157492/1‑251 (MQ=255)
                                                                cGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCgggg                                                                                                                                                              <  1:193330/251‑1 (MQ=255)
                                                                                                                 gCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTg                                                                                                             <  1:191713/251‑1 (MQ=255)
                                                                                                                           aCCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATc                                                                                                     >  1:117640/1‑249 (MQ=255)
                                                                                                                                 aaTGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTg                                                                                             >  1:58446/1‑251 (MQ=255)
                                                                                                                                  aTGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGTCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAGGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTg                                                                                             >  1:10538/1‑250 (MQ=255)
                                                                                                                                        gcattACCCCCTGCCCCTGATAATAACCGCCTGGCACGACAGGTAAAATACTAGTCAGCTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGTTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACg                                                                                      <  1:146969/247‑1 (MQ=255)
                                                                                                                                           aTACCACCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCAGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCAGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGt                                                                                     >  1:250338/1‑249 (MQ=255)
                                                                                                                                                                   cGCCTGGCACGACAGGTAAAATACTAGCCAGTTTGCCGTGAAACCCCTGCCCAGGTAGCGCGATAAACATCACGTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTGTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACa                                                           <  2:50295/251‑1 (MQ=255)
                                                                                                                                                                    gCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGAc                                                            >  1:110625/1‑249 (MQ=255)
                                                                                                                                                                                  gTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTATCGCGTTAAACATCGCGTCCGCATCATCGCCATGTTGCAGACGTAACAGCGAGTTTTGCCGAAATTTGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCTGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTGCCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTta                                            <  1:25746/251‑1 (MQ=255)
                                                                                                                                                                                   tAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTtat                                           >  1:62015/1‑251 (MQ=255)
                                                                                                                                                                                            aGTCATTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACGTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTGTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCt                                   <  2:80651/250‑1 (MQ=255)
                                                                                                                                                                                                                ccTGCCCAGGTAGCGCGGTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCATCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCGCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAg              <  1:288978/251‑1 (MQ=255)
                                                                                                                                                                                                                           aGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGCCGTAACAGCGCGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACATGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCACGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTg   >  2:283618/1‑251 (MQ=255)
                                                                                                                                                                                                                            gcgcCTTAAACATCACTCCCGCATCAGCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAAGTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGc  <  2:110625/251‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              
GAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGC  >  NC_000913/3770307‑3770777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: