Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,176	T→G	E48A (GAG→GCG)	glyQ ←	glycine‑‑tRNA ligase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,176	0	T	G	100.0%	80.7 / NA	31	E48A (GAG→GCG)	glyQ	glycine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/17);  total (14/17)

GGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCA  >  NC_000913/3724935‑3725420                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     ggCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGAc                                                                                                                                                                                                                                             <  1:54376/251‑1 (MQ=255)    ccccAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAgg                                                                                                                                                                                                                                          <  1:294253/250‑1 (MQ=255)                  tttCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTggg                                                                                                                                                                                                                              >  1:204918/1‑247 (MQ=255)                             tCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTAAGAGAACCGAGGTACAGCTCCTGCATATTGACCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGGAGCGACCCACGGTCGGGCGACGCGCAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGAGCAGACGGGTCATTGGGAGAGAGGTTCCCGCGcc                                                                                                                                                                                                                 >  2:179234/1‑251 (MQ=255)                                      aaaCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCAt                                                                                                                                                                                                        <  1:66328/251‑1 (MQ=255)                                        aCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGt                                                                                                                                                                                                      >  1:75954/1‑251 (MQ=255)                                          ggATGTCGTGAATAGTCGGGTCCATGCCCCGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCATCGGGCGCAGACAGGTCATTGGGTGGGAGGTTCCCGCGCCGACTTCCACGTcc                                                                                                                                                                                                    >  2:40773/1‑251 (MQ=255)                                            aTGTCGTGAATAGTCGGGTCCCTGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACAAAAGCAGCCGCCCTCGGTTCTGGCCCCAGCGCGCGCAGACAGGGCATTGGGTGAGAGGTTCCCGCGCCGACTTccctatccc                                                                                                                                                                                                   >  2:294376/1‑243 (MQ=255)                                              gTCGTTAATAGTCGGGTCCAAGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATGGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACAGAAGCAGCAGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGCCCAAt                                                                                                                                                                                                 <  2:288902/250‑1 (MQ=255)                                                               ccATGCCCAGCTCTTTCAGAGCCCCGAGGTACAGCTCCTGAATATTGTCCGGCGGTGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGCACATAAGCAGCCGACATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGGCTTCCATGTCAAATGGTTGACCAATGGtac                                                                                                                                                                                 >  2:198806/1‑247 (MQ=255)                                                               ccATGCCCAGCTCTTTCAGAAAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAg                                                                                                                                                                               <  2:49289/251‑1 (MQ=255)                                                                 aTGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGcc                                                                                                                                                                             >  1:42286/1‑251 (MQ=255)                                                                                  agaACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCCCCTGGAACTGATAGTAGGGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTaa                                                                                                                                                             <  2:262245/250‑1 (MQ=255)                                                                                  agaACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTaa                                                                                                                                                             >  1:262245/1‑250 (MQ=255)                                                                                                  cccTGAATATTGTCCGGCGATGTCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAATGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGAt                                                                                                                                            <  1:285473/250‑1 (MQ=255)                                                                                                      gAATATTGTACGGCGATGGCTTAATGACCACCTGCAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCGGGCCCCAGCGCGCGCAGACAGGCCATTGGGTGAGGGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCGGGCGAGCCCAGTAATCCTGTAAGGTCAgaataa                                                                                                                                          >  2:268321/1‑244 (MQ=255)                                                                                                          atTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCt                                                                                                                                    <  1:32560/251‑1 (MQ=255)                                                                                                            tGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTg                                                                                                                                   <  1:179234/250‑1 (MQ=255)                                                                                                                            aaTGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCa                                                                                                                   <  1:143531/250‑1 (MQ=255)                                                                                                                                    ccTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGCTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTTTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGc                                                                                                          >  2:37599/1‑251 (MQ=255)                                                                                                                                                     ggcggTAAACGGTTGGGGTTTTCGCCGGTGCGACCATCGTTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAACCTTTTGCATATTATTTCGTGCTgg                                                                                         <  2:47303/247‑1 (MQ=255)                                                                                                                                                       ctataaaaggatGGGGGTTTCGCCGTAAAGACCATCGGTCTGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCACCAGAGCGCGCAGACAGGTATTTGGGTGAGAGGTTCCCGCGCAGACTTCCATGTCCAATGGTTGATTAAGGGAGCAGCCCTGGCGAGCACAGAAATGATGAAAGGTAAGGATCAATCACTGGCAGGGCCTGGTTTCGAAATTTTGCATATTATTTCGGGCGGGAt                                                                                       >  2:97807/12‑251 (MQ=255)                                                                                                                                                           aaaaggTTGGGGTTGTCGCCGTAGCGACCATCGGGCGGGCGACGAGAAGGCTGAACATAAGCATCCGCCATCGGTTCTTGCCTCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGTTCAGGATCAAGCCCTGGAAGGTCTTGGTATCAAACTTTTGCATATTAATTCGTGCTGGATACg                                                                                    <  2:166075/246‑1 (MQ=255)                                                                                                                                                                     ggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGATGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAAGCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTaa                                                                         <  1:170023/251‑1 (MQ=255)                                                                                                                                                                                          cggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCg                                                    <  1:176937/251‑1 (MQ=255)                                                                                                                                                                                           ggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGc                                                   >  1:195515/1‑251 (MQ=255)                                                                                                                                                                                                 gCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGAGCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTTCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggatg                                             <  1:258448/251‑1 (MQ=255)                                                                                                                                                                                                      gagaAGGCTGAACATAAGCAGCCGCTATCGGGTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGGTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGa                                        <  1:62557/251‑1 (MQ=255)                                                                                                                                                                                                                     aaGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAAc                         <  1:40773/251‑1 (MQ=255)                                                                                                                                                                                                                      atcagcCGCCCTCGGTTCTGGCCCCTGCGCGAGCAGACCGGTCATTGGGAGATAGGTTCCAGCGCCGACTTCCATGTCCACTGGTTGAACAATGGTGCCGCCCTGGCAAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGTAAGGTCCTGGTCTCAAACTTTTGTATATTATTTCTTGCTGGATACGTTTGGATTTAAAGGTATGGATCAGTATACCCGCTGGATGAAAGATATACAGTCCGAGACg                        >  2:188559/3‑251 (MQ=255)                                                                                                                                                                                                                                            cccAGCGCGCGCAGACAGGTCATTGGGTGAGCGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGCACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGCTCACGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGCTTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCCGGGCTTAACGcc  >  2:155753/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     GGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCA  >  NC_000913/3724935‑3725420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑