| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,647,428 | C→T | A377V (GCG→GTG) | gor → | glutathione reductase (NADPH) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,647,428 | 0 | C | T | 96.0% | 56.4 / ‑6.0 | 27 | A377V (GCG→GTG) | gor | glutathione reductase (NADPH) |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (13/11); minor base G (0/1); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 | |||||||||||
TTGAAGGTATTTACGCGGTGGGCGATAACACGGGTGCAGTGGAGCTGACACC‑GGTGGCAGTTGCAGCGGGTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGT > NC_000913/3647182‑3647670 | ggtcaGGTATTTGCGCGGTGGGCAATAACACGGGTGCGGTTGAGCTGACCCC‑GGTGGCAGTTGCATCGGGTGGCCGTCTCTCTGAACGCCTGGTAAATAACAAGCGGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGAGTGGTACTGTTGGGTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTACACCGTGAt < 2:133442/246‑1 (MQ=255) gAAGGTATTTACGCGGTGGGCGATAACACGGGTGCAGTGGAGCTGACACC‑GGTGGCAGTTGCAGCGGGTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGt > 1:63305/1‑250 (MQ=255) aTTTCCGCGGTGGGCGTAAACACGGGTGCAGTGGGGC‑GGCACCCGGTGGCAGTTGCAGCGGGTCGCCGTCTCTTTGAACGCCTGTTTAATAAAAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTTCTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCTCCGTGATGTATAccg < 2:263798/250‑1 (MQ=255) ccacgGTGGGCGATAACACGGGTGCAGTGGAGCTGACACC‑GGTGGCAGTTGCTGCGGGTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGCTTACAGCACCATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTCTGGCGAC‑GGTCAAGTGAAAGGGTATAAATCCTCTTTCACCGTGATGTTAACCGCCGTc > 2:17769/4‑251 (MQ=255) cgcgGTGGGCGATAACACGGGAGCAGTGGAGCTGACACC‑GGTGGCAGTTGCAGCGGGTAGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGGCTTCAGCCATACGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGCGAAAGTGGTGAACTCCTCTTTCACCGTGATGTGTACCGCAGTcc > 2:114274/1‑250 (MQ=255) ggTGGGCGATAACCCGGGTGCAGTGGAGCTGACACC‑GGTGGCAGTTGCAGCGGGTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCGTTCACCGTGATGTATACCGCCGTcacca < 1:263454/251‑1 (MQ=255) gATAACACGGGTGCAGTGGAGCTGACACC‑GGTGGCAGTTGCAGCGGGTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGAAGGCGCGCGAGCAGTATGGCGAC‑AATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTAATGTATCACGCCGTCACCCCTCACCg > 1:18443/1‑251 (MQ=255) acC‑GGAGGCAGTTGCAGCGGGTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGGTGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGAGAAAGGGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCCATCACCGCCAGCGGTGCCGCATGAAGCTGgtat > 2:264235/1‑249 (MQ=255) c‑GGTGGCAGTTGCAGCGGGTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTTTGc < 1:225390/251‑1 (MQ=255) cAGTTGCAGCGGGTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTgg > 2:224724/1‑250 (MQ=255) ggggTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGgaagag < 1:171269/250‑1 (MQ=255) gCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGAtt > 1:289526/1‑251 (MQ=255) ttAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTgg < 1:151561/250‑1 (MQ=255) gCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATgg > 1:154420/1‑251 (MQ=255) aTGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGa > 1:113733/1‑250 (MQ=255) aGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATg > 1:146340/1‑251 (MQ=255) cccGACCGGGGTCTTCAGCCATCCGCCGATTGTTACTTTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGgcg < 1:232863/249‑1 (MQ=255) cgcccGTGGTCTTAAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTg < 1:227809/248‑1 (MQ=255) aGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGa > 1:67376/1‑142 (MQ=255) gtgtGGTCCTGTTCGTTTAACGGAACCGCGAGCGCGCGATCATCATGGCGCT‑AATCAGGTGAACGTTTAAAAATCATCTTTCGCCGTGTGGTATACCGCCGTCACCACCCACCGCCATCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGTGGCAACCAAAAAAgtct < 2:252730/248‑4 (MQ=255) attggtaagggtGGTTGAACGGAACCGCAGGCGCGCGACCAGT‑TGGGGAAAGATCGGGGGAAAGTTTATAAACCCTTTGTCCCCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACtt < 2:228089/240‑1 (MQ=255) attGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAAGCCTCTTTCACCGTGAGGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACa < 1:17769/248‑1 (MQ=255) aGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAAcg > 1:98282/1‑251 (MQ=255) ataAATCCTCTTTCACCGAGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCACCCACACAAGACTTCGACAATACCGTCGCCATTAACCCAACGGCGGAAGAAGAGTTCGTGCCAATGCGTTaactattaa > 2:273758/1‑242 (MQ=255) cccTCTTTCACCGGGAGGTATCCCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTa < 1:273758/250‑1 (MQ=255) ctTTCCCCGTGATGTATACCGCCGTCACCACGCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTGTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAag < 2:113733/249‑1 (MQ=255) atCACCGTGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCAGCATGAAGCTGGTGGGCGTTGGATCGGAAGAGAAGAATGTCGGTATTCACGGCATTGGCTTTGGTATGGCCGAAATTTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGTAACCACAAAAGACTTCGACAGTACCGTCGCCATTCACCCACCGGCGGCAGAAGGGTTAGTGACCATGCGtaagatttataagggtaaaggga > 2:251541/2‑228 (MQ=255) | TTGAAGGTATTTACGCGGTGGGCGATAACACGGGTGCAGTGGAGCTGACACC‑GGTGGCAGTTGCAGCGGGTCGCCGTCTCTCTGAACGCCTGTTTAATAACAAGCCGGATGAGCATCTGGATTACAGCAACATTCCGACCGTGGTCTTCAGCCATCCGCCGATTGGTACTGTTGGTTTAACGGAACCGCAGGCGCGCGAGCAGTATGGCGAC‑GATCAGGTGAAAGTGTATAAATCCTCTTTCACCGCGATGTATACCGCCGTCACCACTCACCGCCAGCCGTGCCGCATGAAGCTGGTGTGCGTTGGATCGGAAGAGAAGATTGTCGGTATTCACGGCATTGGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGT > NC_000913/3647182‑3647670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |