Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,527	C→T	E405K (GAA→AAA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,527	0	C	T	100.0%	53.7 / NA	21	E405K (GAA→AAA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/15);  total (6/15)

CGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTG  >  NC_000913/822281‑822770                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ggggCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTAcc                                                                                                                                                                                                                                                   <  1:53342/250‑1 (MQ=255)                            aTCAGATAGCTGACGACCACCAGCCCGACCGCACCAAGCATCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCAGTGTCGGTtgt                                                                                                                                                                                                                        <  2:195890/251‑1 (MQ=255)                                        acgacCCCCAGCCCGACCGCACTAAGAAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGCCCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTTTGCGTGCGGATCa                                                                                                                                                                                                            <  2:278009/251‑1 (MQ=255)                                          gacCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAAc                                                                                                                                                                                                          <  1:286350/251‑1 (MQ=255)                                            ccaccaGCCCGACCGCACTAAGCAGCGCAACTCGATTGTAGTCGCGGATGACCTTCCAGACCTCTGCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACTCAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGTTCCTGCGTTTCCATTGTCTGTTGTGCGGGCGGATCAACCt                                                                                                                                                                                                        <  1:265419/251‑1 (MQ=255)                                                         cGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTgg                                                                                                                                                                                           <  1:82483/251‑1 (MQ=255)                                                                                       gcgGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAgcgc                                                                                                                                                            <  1:198355/251‑1 (MQ=255)                                                                                        cgtattcccttcaataCCTCATCCAAGTCCACTGTTTTGGCGTAGACCACCCGCAACACAATCACCTCGATAAAAAACAGCCAGGTGAGGATCGTCTTTGCGAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTAGCCCCTTGGTTTCAGTTTTTACCCGATCCTGCGGTTCCATTGTCGGGTGGGCGTGCGGATCAACCTGCGCCATACGTGGCGTGTGTGC‑CGGAAGCCATCCAACCAgctcc                                                                                                                                                           <  2:135752/236‑4 (MQ=255)                                                                                                              cccAGTCCACTTTTTTGGCGTAGACCACCCGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCGTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGa                                                                                                                                     <  1:289052/251‑1 (MQ=255)                                                                                                                   tCCACTTTTTTGGCGTAGACCACCAGCAACACACTCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGAGGCTGTGATTTACTCATCAAGGTTTTACCCCAGTGTTTTCAGTTTTTACACGATCATGCGTTTCCATTGTCGGTAGTGCGGGCGGATCAACATGCGCCCGACGTAGCGTGTGTACACGGA‑GACAGCCAACCAGCGCCGGGACGTGGCgggagaaagcgaaaacc                                                                                                                                >  1:145256/1‑235 (MQ=255)                                                                                                                            tgggCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCt                                                                                                                       <  1:219175/248‑1 (MQ=255)                                                                                                                                   aGACCACCAGCAACACAAGCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGTGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCGGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCTTTTGGTCa                                                                                                               <  2:271674/251‑1 (MQ=255)                                                                                                                                       caccaGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCCGACGTGGAGTGTGTGC‑CGGAAGCCAACCAACCAACGCCGGGAAGTGGCGAAAAAAGGGGAACGCCAGCCAC‑CTTTTGGTCTGGtt                                                                                                           >  2:211571/1‑251 (MQ=255)                                                                                                                                              aaCACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCGGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCTTTTGGTCAGGTTccaac                                                                                                      >  1:562/1‑247 (MQ=255)                                                                                                                                                       cccGCGAAAAAAACCAGCCAGTAGGGTATCTTCTTTGCTATGCGCCAGCTCGGGTTGGATTTGCCCCACAGGGTTTTACCCT‑GTTTTTGCAGTTTTTACCCGGTCCTGCGTTTCCAGTGTCGGTTGTGCGGGCTGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCATC‑ACGCTTTTGGTCAGGTGCCCCCAGGTGCGTTTgg                                                                                          <  2:133064/250‑1 (MQ=255)                                                                                                                                                                                                   ccAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCaataat                                               <  1:159895/251‑1 (MQ=255)                                                                                                                                                                                                    cAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACctgc                                                                   <  2:261590/230‑1 (MQ=255)                                                                                                                                                                                                    cAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAAAGCCAGC‑ACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACctgc                                                                   >  1:261590/1‑230 (MQ=255)                                                                                                                                                                                                           ggTGTGATTTACTCATCAGTGTTGTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTGTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCa                                       <  1:219239/251‑1 (MQ=255)                                                                                                                                                                                                                   ttACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg                               >  1:174808/1‑251 (MQ=255)                                                                                                                                                                                                                                                tCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCTTTTGGTCAGGTTCCACCAGGAGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATAGTg  >  1:238537/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGC‑CGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGC‑ACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTG  >  NC_000913/822281‑822770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑