Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,439,485 C→T E9K (GAG→AAG)  yhdN ← DUF1992 domain‑containing protein YhdN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,4850CT92.9% 36.6 / ‑3.6 14E9K (GAG→AAG) yhdNDUF1992 domain‑containing protein YhdN
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (5/8);  total (6/8)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

GATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCAC  >  NC_000913/3439269‑3439725
                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  
gATCATCGTGACGGATCCCTTTGAGAATATCCAGAAGCTGAATTGCGTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTTGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCTCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCa                                                                                                                                                                                                                 <  2:145770/251‑1 (MQ=255)
                      gagaAGATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACa                                                                                                                                                                                           <  1:136175/251‑1 (MQ=255)
                               cAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATCCCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTa                                                                                                                                                                                  <  2:48766/251‑1 (MQ=255)
                                                  ctcCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCAGTCTTCAGCAAGCGATACCCCTCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAAGGTTTCGTCGCTACCTGCCAGGTGATCAAACTCACCTTTCGCTCGCGCTTCTGCTATATGGCGCGTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATGGTAGATAAGTGGTGAtact                                                                                                                                                                  <  2:37956/248‑1 (MQ=255)
                                                  ctcCGTTGCCCAAGTTCGGGAGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACTTATTTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTTCCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGAtacta                                                                                                                                                                 <  2:230507/249‑1 (MQ=255)
                                                                               aCCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTCCAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGttt                                                                                                                                  >  2:210719/1‑251 (MQ=255)
                                                                                                                                                        ggTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGCAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTTACGTTTCTTCAGAATACTATGCAGCAGCTTCTGCTTTCTCTTAACGATCAACCAGCTCGATGTACGcc                                                         >  1:125722/1‑251 (MQ=255)
                                                                                                                                                         gTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGGTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGCTCAACCCGCTCGATGTAAGccct                                                       >  2:293889/1‑249 (MQ=255)
                                                                                                                                                            cgccgcTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCgg                                                    <  1:293889/251‑1 (MQ=255)
                                                                                                                                                            cgacgcTACCTGCCAGGTTATCAAACTCACCTTTCTCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCg                                                     <  1:164461/247‑1 (MQ=255)
                                                                                                                                                                          aGGTTATCAACCTCACCTTGCGCTTCCGCTTCTGCTATATGGCGCTTTTCCCACTGGTCAAGTACCACCAAAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTg                                      <  1:236726/251‑1 (MQ=255)
                                                                                                                                                                             ttATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAACTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCAc                                   >  1:224680/1‑251 (MQ=255)
                                                                                                                                                                                      tcaacTTTCGCTAGAGCTTCTGCTATATAGCGCTTTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATCAGAGGGGATACTACGGGTATAAAAAAAACCGCCGGGGCGGGGTTTTTTACGTTGCTTCAGATTACACCGCCGCAGCTTCTGATTTCTCTGAACGGTCAAACCGCTCGATGTAAGACATAGACGCGTTGttccctaccagcaaaccac                          >  2:284923/5‑233 (MQ=255)
                                                                                                                                                                                                              atatGGAGCTTTGCCCACTGGTCAAGTAACCACATAAATACTAAAGGGGATGAACAAAAAGAGTACAACTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTGTTTTTTCGTTGCGTCAGAATACGCTGCAACAGCTTCGGCTTTCTCTGAACGATCAACCAGCTCGCTGTAAACCATCTGCGCGTTTTCGCCGTCACGGCAACCAGACTTCAGAATACGAGGGTAaccac  >  1:68219/1‑251 (MQ=255)
                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  
GATCATCGTGACGGATACCTTTGAGAATATCCAGAAGCTGAATTGCTTCTCTCCGTTGCTCAAGTTCTGGCGGTAAGCAACCGGCATTCTTCAGCAAGCGATACCCCGCACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCAC  >  NC_000913/3439269‑3439725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: