Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,070,8580TG93.5% 92.3 / ‑3.5 31K129N (AAA→AACfbaAfructose‑bisphosphate aldolase class II
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (17/12);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

TGCTCAGTTCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTT  >  NC_000913/3070611‑3071090
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
tGCTCAGTTCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtttt                                                                                                                                                                                                                                       >  1:40724/1‑251 (MQ=255)
             gtATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTcc                                                                                                                                                                                                                           <  1:280375/250‑1 (MQ=255)
               aTGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCAATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCaa                                                                                                                                                                                                                         <  1:148946/250‑1 (MQ=255)
                 gCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAAc                                                                                                                                                                                                                        >  1:53444/1‑249 (MQ=255)
                        cAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTTCTTGTGGCTGTTGTCCCCGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGTCATGCGCTCCAGGTATGTAGATCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACATTTCGATCATGTGAGAAGAGAACATCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTc                                                                                                                                                                                                                <  1:197396/250‑1 (MQ=255)
                                tCCGGCTGGGTGTACAGTGCAGAAGCTTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCa                                                                                                                                                                                                          <  1:135964/248‑1 (MQ=255)
                                    gCTGGGTGTACAGTGCAGAAGCAACCATGTGGCTGTTGTCCACGCCGGCTTCTTCACAAACAGCGAAACCAAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCCCCTGTTATTTAGAGCAGATTTCGTTGTTCTCTTGCAGAACATAATCAGACAGGTCGATCATGTGAGAAGATAACAGAGGCTTCCTCGAAGCTGCGCAGTGGATTTCACCAGCATACAACAGAACGTcactacactgaagc                                                                                                                                                                                                   >  2:179580/1‑238 (MQ=255)
                                          tgtACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCCGTttc                                                                                                                                                                                             >  2:187392/1‑251 (MQ=255)
                                             aCAGTGCAGAAGCGTCCAGGTGGCTGTTGTACACGCCGACTTATTCACCACAGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATAATGTGAGAAGAGAACCGGGGCTTACAGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGAACGTCGATCCACGGCAGCAGTttcttc                                                                                                                                                                                          >  2:33733/1‑251 (MQ=255)
                                              cAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTcg                                                                                                                                                                                         >  1:73762/1‑251 (MQ=255)
                                                gtgaaGAAGCGTCCATGTGGATGTTGTCCACGCCGTCTTCTTCCCAACCAGTGCAAACCCGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGCGATTCTTCAGCCAGGTCGCTCATGTGAGAAGAACAAACCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAAAAGACCATCGATCAACGGCAGCAGTTTCTTcgcg                                                                                                                                                                                       >  2:285524/5‑251 (MQ=255)
                                                                      gttgtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATaa                                                                                                                                                                 <  1:180714/251‑1 (MQ=255)
                                                                             aCGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGCTTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAAAAGACCGTCGATCCACGGCAGCCGTTTCTTCGCGAAGTGGACAGTGTGCAGGATAACCGGAac                                                                                                                                                          >  2:170079/1‑251 (MQ=255)
                                                                              cGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAac                                                                                                                                                          >  1:132451/1‑250 (MQ=255)
                                                                                          cacctcCGGTGCAACACAGTGCGAGTTCCAGAGTCATGCCTATTTTGGACATGCTCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTc                                                                                                                                              <  2:233665/250‑1 (MQ=255)
                                                                                             caacggggCAACCCAGTTCGATTTCCAGAGTAATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGCTTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGAGCATGTGAGAAGAGAACAGCGGCTTACCGGAAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACAGTAGATCCCCGGCAGCAGATTCTTCGCGCAGGGGGCAGTGGGCAGGATAACCGGaaagcaataatgtacaacc                                                                                                                                          >  2:166050/8‑234 (MQ=255)
                                                                                                               cGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGCTGTTCTCTTGCAGAGCTTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATgc                                                                                                                        >  1:180199/1‑251 (MQ=255)
                                                                                                                             aTGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATcgc                                                                                                          >  1:114304/1‑251 (MQ=255)
                                                                                                                                  gATTTTGGACATGCGCTCCAGGTATTTAGAGCAGAGTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGAGCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTGCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCgcgctca                                                                                                     <  2:53444/251‑4 (MQ=255)
                                                                                                                                         gACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATagc                                                                                              >  1:258319/1‑251 (MQ=255)
                                                                                                                                                      ggTATGTAGAGCAGATGTCGATGTGCTCTTGCAGTGATTCTTCAGACAGGTCGATCCTGTGAGAAGAGAACAGCGGCTTGCCGGTAGCCGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGAGCCACGGCAGCAGTTTCGTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGGTCAGCCATCTGGTGAACGGGATGCGCACCAGAGATCGCGCCTAGGATAGCAGCACCCTGCGGa                                                                                 <  2:132451/251‑1 (MQ=255)
                                                                                                                                                                    aTTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGtt                                                                                                                                               >  2:200093/1‑175 (MQ=255)
                                                                                                                                                                    aTTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGtt                                                                                                                                               <  1:200093/175‑1 (MQ=255)
                                                                                                                                                                          aTGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTCACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCttt                                                             >  1:80279/1‑251 (MQ=255)
                                                                                                                                                                                      agagATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATaaa                                                 <  2:258319/251‑1 (MQ=255)
                                                                                                                                                                                       gagaTTCTGCAGACAGGTCGATCATGGGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACGCGCGTTCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCAGCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAACGATAAAg                                                <  2:282562/251‑1 (MQ=255)
                                                                                                                                                                                         gaTTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGa                                              <  1:170129/251‑1 (MQ=255)
                                                                                                                                                                                           ttcttcAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATagcag                                                                                            >  1:14198/1‑203 (MQ=255)
                                                                                                                                                                                           tgcttcAGACAGGTCGAGCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTGTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTGCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATagcag                                                                                            <  2:14198/201‑1 (MQ=255)
                                                                                                                                                                                                           aTCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCTGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCAACGTTGgaaa                             >  1:229499/1‑248 (MQ=255)
                                                                                                                                                                                                                                ggCTTACCGGTAGCTGCTAAGTGGTTTTCACCCTCGTCCAACAGATCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCTCCGTTGGAGAACTTAACGATAACCGGCGCttt       <  2:132251/251‑1 (MQ=255)
                                                                                                                                                                                                                                     aCCGGTAGCTGCGAAGTGGTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGGGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGTTAACCGGCGCTTTCACtt  >  1:962/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
TGCTCAGTTCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCACGGCAGCAGTTTCTTCGCGCAGTGGTCAGTGTGCAGGATAACCGGAACACCATAATGTTCAGCCATCTGGTGAACGTGATGCGCACCAGAGATCGCGCCCAGGATAGCAGCACCCTGCGGAACGTCAGATTTCACGCCTTTACCAGCGATAAAGGAAGCACCACCGTTGGAGAACTGAACGATAACCGGCGCTTTAACTT  >  NC_000913/3070611‑3071090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: