Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,938,548	A→T	T361S (ACG→TCG)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,938,548	0	A	T	85.7%	40.3 / 2.4	21	T361S (ACG→TCG)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (10/8);  total (11/10)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01

CCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTA‑TGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGTAAAAGTTCTCGACGACGCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTG  >  NC_000913/2938324‑2938777                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      ccTGCGAACCGGAGAGCCCGGCAGGGTTGTATAACCCAGGTAGTG‑AACGGCTGGCATCCGGCATGCTGGAATGGGTGAGAAAACTGTTCGGTACGGCGGAAACCCCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATGTATTGTCGGGTCAGAACGCTGGCTGGCAAGGATTGACGCTGAATACCACGCGGGGTCATTCCTATCGCgcggcg                                                                                                                                                                                                             <  2:195145/251‑1 (MQ=255)   ggCGAACTGGATAGCCAGGCAGGGTTGTATAAACCAGGTA‑TGCAATGGCTGGCATACGGCGTGCTGGAATGGGTGAGAAAACTGGTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGgcgcc                                                                                                                                                                                                           <  2:2080/250‑2 (MQ=255)                         ggTTGTATAACCCAGGTA‑TGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTa                                                                                                                                                                                     <  1:292231/251‑1 (MQ=255)                                 accccAGGTA‑TGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTTAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCaa                                                                                                                                                                             <  1:89269/249‑1 (MQ=255)                                             gCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGCCGGCTGAAAAACCCTGGCACAAGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTAGAGGGGGCATTTCTAACGCGCGGCGATGGAAGAGTTAACGGCGCAATTAAAGCGCAATCTACAGATGc                                                                                                                                                                  >  1:100597/1‑251 (MQ=255)                                                  ggctggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGa                                                                                                                                                              >  1:38685/1‑250 (MQ=255)                                                                              gagaAAACTGTTCTGGTCGGCTGCAACAACCTGGCAAATGTTGATTGCAGAAGCTCATCTGATCACGCCTGTCGCGGCTAGCGTAAAAATGCAGTGTGATATATTGTCGTGTCAGAACGCTGCCAGGCAAGGAGTGCCGATTAATACCTCGCGGGGGCTTTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTCAAGCGCAATCTACCGATGCTGGCACAAATCGGTCACCTTAAGACCACTGact                                                                                                                                 >  2:263536/1‑249 (MQ=255)                                                                              gagaAAACTGATCTGGCCGGCTGAAACACCCTGGAAATTGATGATTGAAGACGCTCGTCTGCTCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGAAAGGAGTGACGCTTCATACCTCGCGGGGGCATTTCTATCGCGCGGCGATGGAGGGGTTACATGCGCAATTACAGCGCAATCTACCGATTCTGGAAAACATCGGGCAATTTAAggacactgcat                                                                                                                                 >  2:247864/1‑242 (MQ=255)                                                                                aaaaaCTGTTCAGGACTGCTGATACTATCTGGCAAATGTTGATTGAAGAAGCTCGTCTGAGTGCGCCTGGCGCGGAATGCGTAAAAATGCAGATAGATTTATGGTCGTGTAAGAACGCTGGCTGGCAAGGAGTGACGCTTAATCCCTCGCGGGGGCAGTTCGATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTTCAGATGCTGGAAAAAATCGGGCGCTTTAAGGCCTCTGAatta                                                                                                                               <  2:75921/250‑1 (MQ=255)                                                                                                      aaCACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAAGCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTc                                                                                                         <  2:148855/251‑1 (MQ=255)                                                                                                        cacCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGc                                                                                                       <  1:49004/251‑1 (MQ=255)                                                                                                         acCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGTAGGAAGTCGc                                                                                                       >  1:237704/1‑250 (MQ=255)                                                                                                            cTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCACTTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAac                                                                                                    >  1:213933/1‑250 (MQ=255)                                                                                                                 aaaTGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCAt                                                                                                                                                                                                                            >  2:193492/1‑125 (MQ=255)                                                                                                                   aTGTTGATTGAAGAAGCTCGTCTGATCGCGCATGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATtgtg                                                                                            >  1:160661/1‑251 (MQ=255)                                                                                                                             aagaagCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGTTTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTACCGCGCGGCGCTTGAAGGGGTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGTCTCTGAATTATTGTTAGGCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGAtt                                                                                  <  1:102183/251‑1 (MQ=255)                                                                                                                                 agCTCGTCTGATTGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAg                                                                              <  1:67949/251‑1 (MQ=255)                                                                                                                                                                gTAAAACTGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGACAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGGTAACTGCGCAATTACAGCGCAATCTCCAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGAAGGAAGTCGCAACACATTTTGGAATCAGCTTAAAGCCAATATTCTTGCTTTTCCGGTAAAAGTtct                                               >  2:261954/1‑251 (MQ=255)                                                                                                                                                                                                      tggctggcAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGt                                                                                                                       >  2:127392/1‑141 (MQ=255)                                                                                                                                                                                                      tggctggcAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGt                                                                                                                       <  1:127392/141‑1 (MQ=255)                                                                                                                                                                                                             cAAGGAGTGACGCTTAATACCTCGCGGGGGCATTTCTATCGCGCGGCGCTGGCAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGTAAAAGTTCTCGACGACGCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTg  >  2:153207/1‑251 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      CCTGCGAACTGGATAGCCAGGCAGGGTTGTATAACCCAGGTA‑TGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGTAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCAATCTACAGATGCTGGAAAAAATCGGGCACTTTAAGGCCTCTGAATTATTGTTAGTCGGTGGAGGAAGTCGCAACACATTGTGGAATCAGATTAAAGCCAATATGCTTGATATTCCGGTAAAAGTTCTCGACGACGCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTG  >  NC_000913/2938324‑2938777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑