| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,522,369 | G→A | P37S (CCG→TCG) | xapR ← | DNA‑binding transcriptional activator XapR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,522,369 | 0 | G | A | 90.5% | 51.7 / ‑1.2 | 21 | P37S (CCG→TCG) | xapR | DNA‑binding transcriptional activator XapR |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (10/9); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01 | |||||||||||
CGCCGCATAACCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATT‑TGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTT > NC_000913/2522125‑2522593 | cgccgcATTACCGTGCGCATCCTTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATT‑TGTG‑CAATCCGAGCCAATACAGTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTGGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTg < 1:36994/251‑1 (MQ=255) aaCCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATT‑TGTT‑CAATCCAAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTGAGACatat > 2:58453/1‑251 (MQ=255) cATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATT‑TGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAAcgcg > 1:1716/1‑251 (MQ=255) ccGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTCCTCCCCGACCTATT‑TGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCgct < 1:199666/250‑1 (MQ=255) ccccACATTGCCGTTCCCACTACGCCGAGTTCAATCAGCCCTGCTTCTCCCCGACCTATT‑TGTT‑CAATCCGAGACAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGTTTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGCCATATTT‑AAACGCGCttctgc > 2:160322/1‑251 (MQ=255) ttCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATT‑TGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGc > 1:63668/1‑251 (MQ=255) gCCGTGTTCAATCCGCCCTTCTTCTCCCCGACCTATT‑‑GTGGCAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCg < 2:1716/251‑1 (MQ=255) cAATCCGCCCTGCTTCTCACCGCCCTATT‑TGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCACGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGACTAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGc > 2:53390/1‑250 (MQ=255) aaTCCTCCCTTCTTCACCCCGACC‑ATTGTGTT‑AAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCTAATAAAAAGCTGCGTGCCGAGTTGAGTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGAGCGGCCAAAATGCATCGCTTCCGCTACGGCaa < 2:127026/251‑1 (MQ=255) aaTCCGCCCTGCTTCTCCCCGACCTATT‑TGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCAATTCTTCCATCAAGATTTTGCCCGCGTGTGACAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGACGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAAATCTTCCGCTAAGGCa > 2:173851/1‑250 (MQ=255) tCCGCCCTGCTTCTCCCCGCCCTATT‑TGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGCCGCGATTCTTCCATCAAGATTTTGCCCGAGTGTGTCAGTAAGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGATGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTACCGCTACGGCa > 2:1578/1‑248 (MQ=255) gaccTGCTTTACCCCGCCCGATT‑TGTT‑CAATCCGAGTGAATACATTATGAGCATTGACCAGCAATCGACGCGAGTCATCCATCAAGATGTTGCCCGCGAGGGTTAGTACGACGCTGCGCGAAGGGCGAATAAAAAGCTGCGTGCCGAGTGGATTTTCCATCTCGTTAATATAAATGCAGAGCGAAGGCTGAGACA‑ATTTGAGACGCGCTGCTGCGCGGCCAAAATGCAACTTTTCTGATACGGCAAg < 2:124570/245‑1 (MQ=255) aaTCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGAtctgttctgt > 1:36269/1‑251 (MQ=255) aGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATAcgc < 1:58453/251‑1 (MQ=255) ccAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGt > 1:239589/1‑251 (MQ=255) aTTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCt < 1:260258/251‑1 (MQ=255) cttcCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTAtt > 1:25151/1‑251 (MQ=255) gATTTTGCCCGCGTGTGTCAGTACGTCGCTGCGCGAATGGCGAATAAAAAGTGGCGTGCCGAGTTGATTTTCCATCTCTGTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTAGTAAATTATCAt < 1:2364/251‑1 (MQ=255) gtgtgtCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTAt < 1:263663/251‑1 (MQ=255) gATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACAAAAACACAATGGATAATATACCCGGTa < 1:92761/250‑1 (MQ=255) cAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACAttt > 1:9478/1‑251 (MQ=255) | CGCCGCATAACCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATT‑TGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTGAGACATATTT‑AAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTT > NC_000913/2522125‑2522593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |