Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,310,878	A→T	G219G (GGT→GGA)	ftp ←	FAD:protein FMN transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,310,878	0	A	T	82.3%	32.4 / 4.1	18	G219G (GGT→GGA)	ftp	FAD:protein FMN transferase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (7/7);  total (10/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

GGAGCAATCACCGTCACGGATACCAGATTGTGTTCGATGGGACGCCCGGTTTGCGGATCGATAACATGGGAAAG‑ACGTTTGCCGTCCAGTTCGTAATAGTTACGGTAGCTGCCAGAGGTGCTAATCCCATGACCGTTGATATCCACCACAGCCTGAACCGCGTTTTCTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATACCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGT  >  NC_000913/2310657‑2311121                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    ggAGCAATCACCGTCACGGATACCAGATTGTGTTCGATGGGACGCCCGGTTTGCGGATCGATAACATGGGAAAG‑ACGTTTGCCGCCCAGTTCGTAATAGTTACGGTAGCTGCCAGAGGTGCTAATCCCATGACCGTTGATATCCACCACAGCCTGAACCGCGTTTTCTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATACCACGGCTGTTCAGCGCGCCGCCCACCgc                                                                                                                                                                                                                        >  1:53255/1‑250 (MQ=255)     cAATCACCGTCACGGTTACCAGATTGTGTTCGATGGGACTCCCGGTTTGCGGATCGATAACATGGGACAG‑ACGTTTGCCGTCCAGTTCGTAATAGTTACGGTAGCTGCCAGAGGTGCTAATCCCATGACCTTTGCTATCCACCACAGCCTGAACCGCGTTTTCTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCCGGCCTTCACCCTTCATCCCACGCCTGTTCCGCGCCCCGCCCACCgccccc                                                                                                                                                                                                                    >  2:87028/1‑246 (MQ=255)     aaaTCACCGCCACGGATACAAGTGTGTGTTCGAGGGGACGCCCGGTTTGCGGATCGATAACA‑GGGAAAGGACGTTTGCGTTCCGGGTCTTAAAAGTTACGGTAGCTGCCAGAGGTGCTGATCCCATGACTGTTGATATCCACCACAGCCTGACCCGGGTTTTCTTTATCGGTTGGTTTTTGAATCGTTCCCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACAcc                                                                                                                                                                                                                    <  1:72174/250‑1 (MQ=255)                   gATACCAGATTGTGTTCGATGGGACGCACGGCTTGCGGGTCGATAACATGGGAAAG‑CCGTTTGCCGTCCAGTTCGTAATAGTTACGGTAGCTGCCAGAGGTGCTAATCCCATGCCCGTTGATATCCACCACAGCCTGAACCGCGTTTTCTTTATCGGTTGGTTTTTGAATCTCTACCCGCCACGGCAGGACTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGCCACCACATCTCGGGaaa                                                                                                                                                                                                       >  2:258568/1‑250 (MQ=255)                                                           cGATAACATGGGAAAG‑ACGTTTGCCGTCCAGTTCGTAATAGTTACGGTAGCTGCCAGAGGTGCTACTCCCATGACCGTTGATATCCACCACAGCCTGAACCGCGTTTTCTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACCCCAGATAGCGTGAAATCCCTTCCTGCTCCATCAACCCTGCCAAATGATCGGCCGcc                                                                                                                                                              >  2:61469/1‑250 (MQ=255)                                                             aTAACATGGGAAAG‑ACGTTTGCCGTCCAGTTCGTAATAGTTACGGTAGCTGCCAGAGGTGCTAATCCCATGACCGTTGATATCCACCACAGCCTGAACCGCGTTTTCTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCCTCAAGCGTGCCAACTGATCGGCCGCAt                                                                                                                                                             >  1:155436/1‑250 (MQ=255)                                                                                 tGCCGTCCAGTTCGTAATAGTTACGGTAGCTGCCAGAGGTGCTAATCCCATGACCGTTGATATCCACCACAGCCTGAACCGCGTTTTCTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCAACACCAGATAGCGGGACATCCCTTCCTGCTCCATCACGCGTGCCAAATTATCGGGCCCCTAGCCTTTGCCGCCGGttg                                                                                                                                           >  2:72821/1‑247 (MQ=255)                                                                                            tCGTAATAGTTACGGTAGCTGCCAGAGGTGCTAATCCCATGACCGTTGATATCCACCACAGCCTGAACCGCGTTTTCTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCCTAGCCTTCGCCGACGGTGGAGAGATCGACat                                                                                                                               >  1:227049/1‑250 (MQ=255)                                                                                                             gttcccaagggTGCTATCCCCATGACCTTTGATACCCACACCTGCCTGACCCGTGTTTTCTTTACCGGTGGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCtt                                                                                                             <  1:46194/242‑1 (MQ=255)                                                                                                                    gaggggtaaaTCCCAGGACCGTTGATACCCCCCACAGCCGGAACCGGGTTTTTTTTATGGGTGGGTTTTGGAAGCGCTACCCGCCACGGCAGGCCTTCACCATTCATACCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAg                                                                                                      <  1:87028/243‑1 (MQ=255)                                                                                                                                                            gaacggtgTTTTCTTTACCGGTTGTTTTTTAAACCGCGCCCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGt                                                              <  1:188079/244‑1 (MQ=255)                                                                                                                                                                     tttCTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCAGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTGTTGCAGCTATTGCTGATGCGACTGATTGATGACAGTCAGGTGCTGTAAGCCGGtt                                                     >  1:52405/1‑251 (MQ=255)                                                                                                                                                                        cTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTg                                                  >  1:186963/1‑251 (MQ=255)                                                                                                                                                                              cGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATACCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTc                                            >  1:62254/1‑251 (MQ=255)                                                                                                                                                                                       tttGAATCGCTCCCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTcatcgcatc                                    <  1:72821/250‑1 (MQ=255)                                                                                                                                                                                        ttGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTcatcgcatcg                                   <  1:272899/250‑1 (MQ=255)                                                                                                                                                                                                                    tCACCGTTCATTTCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAAcc        <  1:261263/249‑1 (MQ=255)                                                                                                                                                                                                                        cGTTCATTTCACGGCTGTACAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCTTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGTCtgt  <  1:92611/251‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    GGAGCAATCACCGTCACGGATACCAGATTGTGTTCGATGGGACGCCCGGTTTGCGGATCGATAACATGGGAAAG‑ACGTTTGCCGTCCAGTTCGTAATAGTTACGGTAGCTGCCAGAGGTGCTAATCCCATGACCGTTGATATCCACCACAGCCTGAACCGCGTTTTCTTTATCGGTTGGTTTTTGAATCGCTACCCGCCACGGCAGGCCTTCACCGTTCATACCACGGCTGTTCAGCGCGCCGCCCACCGACACCAGATAGCGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGT  >  NC_000913/2310657‑2311121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑