| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,307,733 | C→T | A295T (GCG→ACG) | yojI ← | ABC transporter family protein/microcin J25 efflux protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,307,733 | 0 | C | T | 87.5% | 60.7 / 0.6 | 32 | A295T (GCG→ACG) | yojI | ABC transporter family protein/microcin J25 efflux protein |
| Reads supporting (aligned to +/- strand): ref base C (3/1); new base T (14/14); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01 | |||||||||||
GATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGAATGT > NC_000913/2307496‑2307975 | gATACAAGCCCGTCAACAACATCGCCATCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑c < 1:102949/251‑1 (MQ=255) cGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGaa > 1:119996/1‑250 (MQ=255) aTTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGCCCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCCCGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGATTTTGTTCAGCTTGTTAAACGTCCCCTGCGCCTT‑CAGCAGCGTCGGCAATGCTCCAACCGCCGaaa < 1:255676/249‑1 (MQ=255) cGCTCCCGTGGCCGCCAATCAGAAACAGCAGCTCGCCACTTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAgcggc < 1:203172/251‑1 (MQ=255) gCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAgcggcg > 1:71634/1‑251 (MQ=255) cccGTTGCCGCAAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCTTT‑ACCCTGATAAGCAAACGTCCCGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTGGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTAc < 1:115736/250‑1 (MQ=255) cccGTGGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATAAATCGGACCAACGGAAAACGCGTT‑ATCCTGAAAAGCAAACGTCACGTTACGCAGCCCCAGCTTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAACCTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTAc < 1:227031/250‑1 (MQ=255) ccGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTACTCGGACCAACGGAAAACGCGTT‑ATCCTGATACGCACACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCATGAGGGCGCGGAAACTCAG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGATTGTTAGACGTCACCTGCGCCGT‑CAGCAGCGTCGGCACTGCGCCAACCGCCGAAAGCAGCGGCGTACg > 1:267814/1‑251 (MQ=255) ccGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTACTCGGACCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGtccg > 1:118330/1‑248 (MQ=255) aTCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAACCGGACCAACGGAAAACGTGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCTGTTGGAAAACGCCTGCGGGCGCGAAAACTCTG‑CTTGGAAAGGCGCGTGCGCTAATTTGTTAAGCTTGTTAAACGTCTCCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAg < 1:282804/251‑1 (MQ=255) cgaaaccgacgcTCTCCACGTTAGATGGGGAAATTAATCGGCGCAAAGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTTCGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGTGAGCGCGAATTTGTTACGCTGGATAAACGTCCCATGCCCCGT‑CAGCAGCGTCGGCAATGAGCCAACCGACGAACAAAGCGGCGTACGCAGAAATacacacg > 2:40527/11‑244 (MQ=255) aCAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTT‑ATCCTTATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCAAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCGGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAAc < 1:98806/251‑1 (MQ=255) agcagcTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACg > 1:33056/1‑250 (MQ=255) ctccACGTTTGAGTGTGAGATTAATCGCACAAACGTAAAAC‑CGTTTATCCTGCTAAGCAACCGCCCCTTTACGCATCTCCAGCTTTTCCCAGTTGGGAAACGCTTGCGGGCTCGTAACCCCTG‑TTTTAAAAGGCTTGAGCGCTAATTTTTTCATCTTGTTAAACTTCCCCTGAGCCGA‑CAGCATCGGCGTCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAgt < 1:41877/247‑2 (MQ=255) cacctcgttgaaTGGTGAGATTAATCGAGCCAACGAAAAACTCGTT‑ATCCGTATAAGCAAACGTCGCGTTCCGCAGCTCCAGCGTTTGCAAGTTGGGAAACGCCTGCGGGCGCGGAACCTCTC‑CTTTAAAAGGCCCGAGCGCAAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTc < 1:211488/240‑1 (MQ=255) cGGACCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAAc > 1:112481/1‑251 (MQ=255) cggaaaaaCGAGTT‑AACTTGAAAAGCATACGACACGTTACGCAGCCCAATCGTTTGCAAGTGGGGAAACGCCTGCGGGCGAGGAAACTCTG‑CTTAGAAAGGCGAGAGCGCGATGTTGTTCAGCTTGTGAAACGTCACTTG‑GCCCTGCAGCAGGGTCGAAAAGGCCCAAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCTCGGCAACGTTGGTATCAGCCCAACCGAGGCt < 2:267630/247‑1 (MQ=255) cgTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATc > 2:102418/1‑251 (MQ=255) gTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCACCGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATcc > 2:284155/1‑251 (MQ=255) tGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAacac > 1:6377/1‑251 (MQ=255) ccaaccgcccctttccGCACCTCCAGCTTTTGCCAGTGGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGcc < 1:102418/236‑1 (MQ=255) acAAACGTCATGTTACGTAGCTCAAGGGTTTGCCAGTGGGGAAACGCTTGCGGGCGTGGAA‑CTTTGTCTTTGAAAGGCGCGAGGGCGAATTTGTTCACCGTGTAAACCGCCACTTGTGTCGC‑AAGAAGCGTGGGCAAGGCGCCCACTGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGcc < 1:257726/250‑1 (MQ=255) cACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTgcgc > 1:36787/1‑251 (MQ=255) cAGCTCCAGCGTTTGACAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGACAGGAGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAACGCGTCAACGAATAGGTCGAGGCAACGTTGGTATCAGCCCAACCGAGACTGTTCGCCATCCAGAACACCAAGCCGATTGCGccccgcctc > 2:241642/1‑244 (MQ=255) cgCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGCAACTATG‑CTTTGAAAGGCGCGAGAGCGAATTTGTTCAGCTTGTTAAACGTCCCCTGCGCCGT‑CAGCAACGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGCATAGGTCGCGGAAAAGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGAGGtt > 2:29030/2‑251 (MQ=255) ttGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGCAAGGCGCGAGCACGAATTTGTTCAGCTTATTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGAACGCAGGAATAAAAGCGCCACCGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGATGTTCGCCAGCGAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTggcc > 1:176785/1‑248 (MQ=255) aGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGCAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGAAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGACGCGGCCACGTTGGGATCAGCCCAACCACAGCTGTTCGCCCTCCAGAACACCAGGCCGATTACGCCCAGCATCATGATGTTTGCCCAGTTc > 2:150989/1‑251 (MQ=255) ccggCGGGGGCGGAAACTCTG‑CTTGAAAAGGGGCGAGCGCGAATTTGTTCAGCTTTTTAACCGTCGCCTGCGCCGT‑CATCGGCGTCGGAAAGCCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGa < 1:108176/248‑1 (MQ=255) ctG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGTCACCTGAGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACAAGTTCACGGCACTAAGATGGAAGGTGTATGcgcg > 1:262781/1‑250 (MQ=255) tGTTCAGCTTGTGAAACGTCGCCTGCGCCGT‑CAGCAGGGTCGGAAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTgcgc < 1:94157/250‑1 (MQ=255) agTTAAACGTCACCTGCGCCGT‑CAGCAGCGTCGTCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGa < 1:73980/247‑1 (MQ=255) ttAAACGTCACCTGCGCCGT‑CAGCAGCGTCGGCACTGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCCTCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGCCCAGTTCACGGCACTAAGCAGGACGGTGCCTGCGCGAATAATATGGTGGCGGTACTCTTGCGCATCAGGGATGt > 2:243971/1‑250 (MQ=255) | GATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTT‑ATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAGCGTTTGCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTTGTTCAGCTTGTTAAACGCCACCTGCGCCGT‑CAGCAGCGTCGGCAATGCGCCAACCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGAATGT > NC_000913/2307496‑2307975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |