Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,142,598	G→A	L117L (CTC→CTT)	udk ←	uridine/cytidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,142,598	0	G	A	97.0%	101.7 / ‑3.6	33	L117L (CTC→CTT)	udk	uridine/cytidine kinase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (19/13);  total (20/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

CGATGCGGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCG  >  NC_000913/2142351‑2142841                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     cgATGCGGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCaaga                                                                                                                                                                                                                                                   <  1:52038/251‑1 (MQ=255)   aTGCGGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAat                                                                                                                                                                                                                                                 >  1:70888/1‑251 (MQ=255)     gCGGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTTATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAatga                                                                                                                                                                                                                                               <  1:291832/251‑1 (MQ=255)                 gccgcGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCg                                                                                                                                                                                                                                   >  1:236408/1‑251 (MQ=255)                                   gtacGCATATTGTTTGGAAGGCTCAATGACTTGCAGGAACATCGGGCGCACGGTTTTTTGATCTTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAAGGCTGACCTTCTTCGGCTCAACCGTAACCGttt                                                                                                                                                                                                                 >  2:8701/4‑251 (MQ=255)                                          tatTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTAACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGCTGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCAt                                                                                                                                                                                                          >  1:180116/1‑251 (MQ=255)                                              gTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgc                                                                                                                                                                                                      >  1:183763/1‑251 (MQ=255)                                                               aaTTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCt                                                                                                                                                                                     <  2:280043/251‑1 (MQ=255)                                                                gttGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCCCGCTTGATGCGGCGAATCAGGCAGATATCCAGCGGGGTATCAACGAAAATAGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTAAGCAACAAAATGCCTTCAATAATGATGACCTTCTTCGGCTCAACCGTCAACGTTTCTTTCATACGCGTGTGtttaaaatcgctt                                                                                                                                                                                    >  2:69308/2‑240 (MQ=255)                                                                    cAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTaa                                                                                                                                                                                 >  2:240733/1‑250 (MQ=255)                                                                           aTCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGc                                                                                                                                                                           >  1:254558/1‑249 (MQ=255)                                                                                 cgcACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAAt                                                                                                                                                                   <  2:70888/251‑1 (MQ=255)                                                                                      ggTTTTTGTATATTGCGCCATCTCTGAATCCATTGAACGCCCACTCGCGTTAACGTCACGCTTGATGCGGCGCATCAGTCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCTACATAGCTGTAAACCGGCAGGTCAATTGCCg                                                                                                                                                              <  2:293740/251‑1 (MQ=255)                                                                                           tgtGATATTGCGCCATCACGGAATCCATGGAACGCCCACGCTCGTTAACGTCATTCTTGATGCGGCGCATCAGGCAGAAATCCAGCGGGGTAGCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTGCGTCGCAACCGTCTCCGTTTCTTTCCTACGCGTATGTTTAACATAGTTGTAAACCGGCAGGTCAATTGCCGAG‑cc                                                                                                                                                         <  1:173067/248‑1 (MQ=255)                                                                                                 atTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTc                                                                                                                                                  <  1:159287/251‑1 (MQ=255)                                                                                                                           cGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAAAGCTTGTAAATGCTCAagcagc                                                                                                                          >  2:59401/1‑249 (MQ=255)                                                                                                                           cGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGAGTTTGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAagcagc                                                                                                                          >  2:260580/1‑249 (MQ=255)                                                                                                                                      gTTAACGGCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCa                                                                                                             <  2:39279/251‑1 (MQ=255)                                                                                                                                           cGTCACGCTTGAGTCTGCGCAGCACGCAGATATCCAGCAGGGTAACAACGAAAATGGATAAGTTAAATACGTCACGCAAACGCTCATCCGTCAGCAACAAAATGCCTTCAATAACGATGTCCGTCTTCGGCTCAACCGTCGCCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGTTCACTTGCGTAG‑CCGCGTGTCAACGCCTGTAAATTCTCAAGCAGCTGACTGTGATCCATctcg                                                                                                        <  2:116302/251‑4 (MQ=255)                                                                                                                                                 gCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGCGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCACTCGCGCTgggt                                                                                                  >  1:43308/1‑250 (MQ=255)                                                                                                                                                     gATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCCAGCAGCAGACTGTGATCAATCCAGCTGGGATGGt                                                                                              >  2:248470/1‑251 (MQ=255)                                                                                                                                                         cggcgCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGt                                                                                           >  1:225656/1‑250 (MQ=255)                                                                                                                                                                  aGGCAGTTATCCAGCGGGGAATCAACGAACATGGAGAAGTTAATTTCGTCACTCAAACGCGCATCCGTCAGAAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCCACCGTCACAGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTTCC‑AGACAGCGTTTCAAAGCTTGTAAATGCCCAGAAAGCAGACTGTGATCCATCCCGCTgtgcgggtcgtgggttgcctt                                                                                 >  2:166368/1‑231 (MQ=255)                                                                                                                                                                  aGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCtt                                                                                 >  1:225388/1‑251 (MQ=255)                                                                                                                                                                           tCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGttc                                                                        >  1:13668/1‑251 (MQ=255)                                                                                                                                                                                  gggTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATc                                                                 <  2:236408/251‑1 (MQ=255)                                                                                                                                                                                  gggTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATc                                                                 <  2:212061/251‑1 (MQ=255)                                                                                                                                                                                  gggTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATc                                                                 <  1:246501/251‑1 (MQ=255)                                                                                                                                                                                         aaCGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGAc                                                              >  2:18341/1‑247 (MQ=255)                                                                                                                                                                                                              ttCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGc                                     >  1:246843/1‑251 (MQ=255)                                                                                                                                                                                                                 gTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGCCAATTGACGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGt                                  >  1:273508/1‑251 (MQ=255)                                                                                                                                                                                                                        aaaCGCGCATCCGTCAGCAACAAAATGCCTTCAAGAATGATGACCTGCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCggg                           <  1:177383/251‑1 (MQ=255)                                                                                                                                                                                                                                                aTGCCTTCAAGAATGATGACCTTCTTCGGCGCAACCGTCCCCTGTTCTTTCATACGCGTAGGTTCAACATAGCTGTAAACCGGCAGGGCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCAcc   <  2:225656/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gCCTTCAAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCg  >  1:68043/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     CGATGCGGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAG‑CCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCG  >  NC_000913/2142351‑2142841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑