Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,024	G→T	I162I (ATC→ATA)	wbbK ←	putative glycosyltransferase WbbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,024	0	G	T	100.0%	29.9 / NA	11	I162I (ATC→ATA)	wbbK	putative glycosyltransferase WbbK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (3/8);  total (3/8)

TTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACA‑CTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTT  >  NC_000913/2103780‑2104266                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ttGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATaaa                                                                                                                                                                                                                                                <  1:166243/250‑1 (MQ=255)                          gATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAAttgttgc                                                                                                                                                                                                                       >  1:256026/1‑249 (MQ=255)                                   cAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGTAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACA‑CTg                                                                                                                                                                                                            <  1:10171/251‑1 (MQ=255)                                        aTTTAATCTTGGATTGGTCTTTCAATTTCCTTGCTGCACTAATACTAAGCTCGTCATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAATTTTTTCTTTCATCCAGAATTGTTGAA‑AAACAACTGCAGt                                                                                                                                                                                                        >  2:25656/1‑250 (MQ=255)                                                            ttCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACA‑CTGCAGTATTTTTTTTAATGtttattt                                                                                                                                                                                    <  1:206324/250‑1 (MQ=255)                                                                                                    tAAATACTCGTGGAACAGCAGGATAAAATAATGTCAACTTAGAATGGTTATTCTTAAATTGAGAATTATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTTGCCGACTGACAATGATGTTATTTATAGAATAGTTATTTATAAATTTTTCTTTCATTCATAATTGTTTAACAAACA‑CTGCATTATTTTTTTTAATTTTTATTTTATAGATCAGCCCGTATATCATTTTAAATAAGAAAAAGcc                                                                                                                                            <  2:64255/250‑2 (MQ=255)                                                                                                              gggAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGATAATCATCAGCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTTAACAAACA‑CTGCAGTATTTTTTTTAATGTTTATCTTATATATCAGTCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCAt                                                                                                                                   <  2:77165/248‑1 (MQ=255)                                                                                                                               atatTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACA‑CTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATaa                                                                                                                 >  1:153552/1‑250 (MQ=255)                                                                                                                                           cAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTGGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACA‑CTTCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTAGAGCATTTTAAATAATAAAAATCTAGGCGCCATAAGAATTTCACGGAATAAAATTCCTTTATaa                                                                                                    <  2:256026/251‑1 (MQ=255)                                                                                                                                                                                                                                gTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACA‑CTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTa                <  1:47038/250‑1 (MQ=255)                                                                                                                                                                                                                                              atTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACA‑CTGCATTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACTCATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCttt  <  2:93648/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACA‑CTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTT  >  NC_000913/2103780‑2104266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑