Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,849,629 T→C L265L (TTA→CTA)  sppA → protease IV, a signal peptide peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,849,6290TC92.3% 62.4 / ‑1.1 26L265L (TTA→CTA) sppAprotease IV, a signal peptide peptidase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (12/12);  total (13/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

CTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGC‑TGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTTTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGG  >  NC_000913/1849400‑1849860
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        
ctacAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGC‑TGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGACGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTCACCA‑AAACCGGTGGCGAt                                                                                                                                                                                                                     >  1:5562/1‑250 (MQ=255)
                                     cATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGC‑TGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGc                                                                                                                                                                               >  1:131372/1‑251 (MQ=255)
                                        gtgtTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGCTGATATGTCACCGGCAGCCCGCGAAGC‑TGACAGCCGCTGGATTGGTGAGCTGTGGCAACACTATCTGAATACTGTTGCCGCTAACCGGCCGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCCAGGCTTGCTTGAGGGTCTA‑CCACAAACCGGGGGCGAAACCGCGAAATATGCACTGGAACAAAAGCTGGTCGAAGCACt                                                                                                                                                                            >  2:23843/1‑251 (MQ=255)
                                                   gggTACGTATAAATCTGCCGTTGAACCGTTTCTTCGTGATGATATGTCACCGGCAGCCCGCGAAGC‑TGACAGCCGCTGGATTGGTGAGCTGTGGCAATCCTATCTGAATACTGTTGCCGCTAACCGGCAGTTCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTTGAGCTCCCGCGAAATCTGGACTGGAACACAAACTGGCCGATTCCCTGGCATCGAGTg                                                                                                                                                                 >  2:160924/1‑251 (MQ=255)
                                                           ataAATCTGCCGTGGACACGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGC‑TGACATCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTCACCGGCAGATCCCTGATGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑TAACCCGTGGCGATACCGCGAAATATGCACTGGCAAACAAGCTGGCAGATGCGATGCCACCGAGCGCGGAAATc                                                                                                                                                         >  2:215941/1‑251 (MQ=255)
                                                            aaaaTCTGCCTTTGAACCGTTTATTCGTGATGATATGTCCCCTGCAGCCCGCGAAGCGTG‑CAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATGCTGTTGCCGCGAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGTGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATAGGCACCGGAAAACAAGCTGGTCGATGCACTTGCATCGAGTGTGGAAATCg                                                                                                                                                        <  2:152836/250‑1 (MQ=255)
                                                                                      gTGATGATATGTCACCGGCAGCCCGCGAACC‑TGACAGCCGCTGGATGGGTGAGCTGGGGCAAAACTACCTGAATACTGTTGCCGCTAACCGGCAGATCACTGCTGATCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGCGGGTCTAACCA‑AAACCGTTGGGGTTACCGCGAAATAATCACTGGCAAACAAGCTGGTCGATGCACTCGCATCGAGTGCGGCAATCGAAAAAGCACTGaaacaacgattgtgc                                                                                                                              >  2:31036/1‑237 (MQ=255)
                                                                                           gatATGTCACCGGCAGCCCGCGAAGC‑TGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTAGCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTTTAACCA‑AAACCGGTGGCGATACAGCGAAATATGCACTGGAAAACAAGCTGGTCGTTGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAg                                                                                                                         <  1:152292/251‑1 (MQ=255)
                                                                                                                 acgC‑TGACAACCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTACCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACAGGAGGCGCTACCGCGCTATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGGGCGGAAATCGAAAAAGCACTGACCAAAAAATTCGGCTGGAGTAAGACTGATAAAAATTATccc                                                                                                   >  2:272358/3‑249 (MQ=255)
                                                                                                                 aaGC‑TGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATcgc                                                                                                   <  1:231256/251‑1 (MQ=255)
                                                                                                                      tGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGcc                                                                                                >  2:77575/1‑250 (MQ=255)
                                                                                                                        aCAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATc                                                                                             <  1:23843/251‑1 (MQ=255)
                                                                                                                            ccgtttgattgggtggCTGTGGCTAAACTATCTGAATACTGGTGTCGCTAACCGGAAGATCCCTGCGGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTGGAGGGGCTAACCA‑AACGCGGTGGCGTTACCGCGAAATATGAACTGGAAAACAAGCTGGGCGAGGTATTGGCATCTAGGTAGGAAATCGAAAACGCACGGTCCAATGAAGTCTGCTGGAGTAAGACTGATAAAAAGGAGCGGGCCATCAGtt                                                                                         <  2:136296/236‑1 (MQ=255)
                                                                                                                            ccGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGtt                                                                                         >  1:166587/1‑251 (MQ=255)
                                                                                                                                              gtgGCAAAACTATCTGAATACTGTTGCCGCTAACCGGTAGATCCCTGCTGAGCAGGTATGCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCATAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCAtt                                                                         <  2:198721/249‑1 (MQ=255)
                                                                                                                                                 gCAAAACTATCTTAATACGGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCGTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGCTTACGCATTGaaaa                                                                    <  2:138195/251‑1 (MQ=255)
                                                                                                                                                  cAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCACAGAATTCGGCTGGAGTAAGACTGATAAAAATTCTCGCGACATCAGTTATTACGATTACGAATTGAAAAc                                                                   >  2:202002/1‑251 (MQ=255)
                                                                                                                                                  cAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAAc                                                                   <  1:212980/251‑1 (MQ=255)
                                                                                                                                                         aTCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGATATTACGATTACGCATTGAAAACGCCGGc                                                             <  1:183243/250‑1 (MQ=255)
                                                                                                                                                         aTCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCGTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGc                                                             <  1:272358/250‑1 (MQ=255)
                                                                                                                                                                    gTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGaaaa                                                                                                                                                    >  1:105780/1‑152 (MQ=255)
                                                                                                                                                                    gTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGaaaa                                                                                                                                                    <  2:105780/152‑1 (MQ=255)
                                                                                                                                                                           cTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGATGCTTGAGGGTTTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGCTTACGCATTGAAAACGCCGGCCGATAcatggtacagcatc                                          >  2:78800/1‑238 (MQ=255)
                                                                                                                                                                                         ccTGCTGGGCAGGGATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAAACA‑AAACCGGGTGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGc                            <  2:160519/251‑1 (MQ=255)
                                                                                                                                                                                              ggAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATg                       <  1:244164/250‑1 (MQ=255)
                                                                                                                                                                                                                   gcAAGGGTTGCTTGAGGGTCTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTAAGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAgg  >  1:156118/1‑251 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        
CTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGC‑TGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCCCTGCTGAGCAGGTATTCCCTGGCGCGCAAGGGTTGCTTGAGGGTTTAACCA‑AAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGG  >  NC_000913/1849400‑1849860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑