| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,767,014 | C→T | G558S (GGT→AGT) | ydiJ ← | putative FAD‑linked oxidoreductase YdiJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,767,014 | 0 | C | T | 87.5% | 50.7 / 1.9 | 24 | G558S (GGT→AGT) | ydiJ | putative FAD‑linked oxidoreductase YdiJ |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (8/13); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-02 | |||||||||||
TGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGT‑CAAATGCCGCTTTCACTTTGCGCAGTTCTG‑CAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAA > NC_000913/1766802‑1767258 | tGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCAATTTCATCATCGGCGCATCg > 1:197983/1‑250 (MQ=255) gATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCgaga < 1:270756/251‑1 (MQ=255) aCGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTc < 1:105877/251‑1 (MQ=255) aTCCGCCAACAACCGAAGCCATCCACGCACCAGGTTTGCGCGCCCTTTCGGGGAATGAAATCGGTGGTGGGTGATCTGCATCGACGGACACATCGGACTCCGGGCAGCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACGGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCTCCGCGTCCACTTTCATCATCGGCGTATCGAGACCTTCTGGCgg < 2:131313/249‑1 (MQ=255) ccAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGATCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGGACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGTCCTTCTGGCGGGCATATc > 1:105159/1‑251 (MQ=255) acaaACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTc < 1:65945/251‑1 (MQ=255) gCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGAGCGAATGTACTGCGCTTCACCGCGTcc < 1:234955/197‑1 (MQ=255) cgctcccTTGCGGTGAATGAATCCGGTTCGGGGGTATCTTCATCGACGGACACATCGGACTCCGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTCCCGCAATGGGTATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGT‑CAAAt < 2:13715/247‑1 (MQ=255) aatgaatCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCCTCATCGGCGCATCGAGAACTTCTGGCGGGCAAATCTTCCCTGGGTTTAGTCGGTTAAGCGGGT‑CAAATGCCGCATTCCCtttt > 1:38480/1‑250 (MQ=255) atgactCCGGTTAGGGGTAATCTTCATCGACGGACGCATCGGTCTACGGGATTCAAAGTTGAAGCATAAACCGTGGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGTGATCTGCCGACTGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGTGCAAATCTCCCCTGGGTGGAGTCGGTTATGCGGTT‑CAAATGCCGATTTCACTTTgc < 2:140783/251‑1 (MQ=255) tCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATATTCCCTGGGGTGAGTCGGTTATGCGGGT‑CAAATGCCGCTTTCACTTTGCGCAGt > 1:187571/1‑251 (MQ=255) cGGTTCTGGGTGATCTTCATCGACGGACCCATCGGACTACGGGCATCAAAGTTGAAGCATAACCCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTgg < 2:33325/203‑1 (MQ=255) cGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTgg > 1:33325/1‑203 (MQ=255) ttctggggtagtctcctCGACGGACACCTCGGACTCCGGGCATCAACGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCGGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCCCCGCGTCCTCTTCCATCAGCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGT‑CAAATGCCGCTTTCACTTTGCGCAGTTCTg < 2:57825/234‑1 (MQ=255) cgtaCTACGGTCACCATAGTCGAAGCCTAAACCGTGGCCGTTACACTCCACCGCAACGCGCCACTGCCGGCTTACCGCAAGGGGGATCTGCCGAGCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGTT‑CAAATGCCGCTTTCGCTTTGCGCAGTTCTG‑CAAAAAGTTCCTCACCGAAAAACGCCggc < 2:82819/247‑2 (MQ=255) aCTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATATTCCCTGGGTTGGGTCGGTTATGCGGGTACAAA‑GCCGCTTTCACTTTGGGCGATTCTGACAAAAA‑TTCCTCAcacaaaaccgacagtgtgt > 2:216363/1‑233 (MQ=255) cAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGT‑CAAATGCCGCTTTCACTTTGCGCAGTTCTG‑CAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGcgcg < 1:165100/250‑1 (MQ=255) aaGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTc > 1:69940/1‑134 (MQ=255) aaGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTc < 2:69940/134‑1 (MQ=255) aaCCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGGTGAGTCGGTTATGCGGGT‑CAAATTCCGCTTTCCCTTTGCGCAGTTCTG‑CAAAAAGTTCCTCCCAGAAAAACGCCGTGCTTTCTTCAGCGCGAAAACCTTTGCCGTGc > 1:2096/1‑251 (MQ=255) gctgGCGTACCGCAATGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGT‑CAAATGCCGCTTTCACTTTGCGCAGTTCTG‑CAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAgc < 1:108423/251‑1 (MQ=255) aaTGGGGATCTGCCGATCGAATGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGT‑CAAATGCCGCTTTCACTTTGCGCAGTTCTG‑CAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTACTTCGCAGTCAGCGCCACCACGtcat < 1:216363/251‑1 (MQ=255) ggATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGT‑CAAATGCCGCTTTCACTTTGCGCAGTTCTG‑CAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCag > 1:64134/1‑249 (MQ=255) tGTACTGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGT‑CAAATGCCGCTTTCACTTTGCGCAGTTCTG‑CAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCaaa > 1:203218/1‑250 (MQ=255) | TGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCGGCGCATCGAGACCTTCTGGCGGGCAAATCTTCCCTGGGTTGAGTCGGTTATGCGGGT‑CAAATGCCGCTTTCACTTTGCGCAGTTCTG‑CAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTATTTCGCAGTCAGCGCCACCACGTCATCAGAGATTTGCTTCATCAAA > NC_000913/1766802‑1767258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |