Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,667,4600GA95.7% 64.6 / NA 25Q369* (CAG→TAG) mlcDNA‑binding transcriptional repressor Mlc
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (13/9);  minor base C (1/0);  total (15/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGT  >  NC_000913/1667233‑1667695
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
gTTAGGCTTCACAAATATAAACCAGGCAAATAATTAACCTTGAACGTATAAGTTATGCTTTCCTGGCTCAAATTTAGCTAGCGCAATTTTTGGTAGTACAGTTAAAACATGCTAACCCTGCAACAGACGCATCAACAAAGAACCGTTATACATCGCGTCTTTTCCCAGTGCAGCGCCTGCCCTCATGCCATGGATAGAAAACTGAGTACTCTCAAAGCCGAATTCCTCAC‑TATTCGCCGGAAGGGCctgct                                                                                                                                                                                                                      >  2:76414/1‑251 (MQ=255)
          acaAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCt                                                                                                                                                                                                            <  1:163950/251‑1 (MQ=255)
            aaaTATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAACCTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGt                                                                                                                                                                                                          >  1:18779/1‑251 (MQ=255)
            aaaTATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGt                                                                                                                                                                                                          >  1:38224/1‑251 (MQ=255)
                                     ccTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAACATGTTAACCCTGCAACAGAAGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAAAGCTGATGTGCT‑ACATATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATc                                                                                                                                                                                 >  2:120994/1‑251 (MQ=255)
                                         gAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGcc                                                                                                                                                                             >  1:226363/1‑251 (MQ=255)
                                          aaaGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCg                                                                                                                                                                            <  2:38224/251‑1 (MQ=255)
                                                                       aTTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAtt                                                                                                                                               >  1:31560/1‑251 (MQ=255)
                                                                        ttGAGATAGCGCAAATTTTGGTAGAAAAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGCTGACCGGGACGAGGATATCTGCCGCTTTACTTACCGGTGAGCCAATCAGTAttt                                                                                                                                              >  2:140471/1‑251 (MQ=255)
                                                                         tGAGAGAGCGCAAATTTTGGTAGAAAAGTTAAAAAATGTTACCCCTGCAACAGACTAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTAttt                                                                                                                                              <  2:244743/250‑1 (MQ=255)
                                                                                              aGAACAGTTAAAAAATGTTAACCCTGCAACAGCCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                                                                        >  2:217491/1‑251 (MQ=255)
                                                                                                       aaaaaaTGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGc                                                                                                               >  1:74079/1‑251 (MQ=255)
                                                                                                                  aCCCTGCAACAGACGAATCAACAAAGCACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGcc                                                                                                    >  1:120954/1‑251 (MQ=255)
                                                                                                                    ccTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACACTGATGGCAAGACTGCGCCCg                                                                                                  >  2:52106/1‑251 (MQ=255)
                                                                                                                                      acaaAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCg                                                                                <  1:133533/251‑1 (MQ=255)
                                                                                                                                              ccGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatgat                                                                        <  1:133714/251‑1 (MQ=255)
                                                                                                                                                 ttATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTCCTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTACTGATGTc                                                                     >  1:214283/1‑251 (MQ=255)
                                                                                                                                                      cATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGCCAACTGAGTACTCTAAACGCTGCTGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTAATTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCAGTAATGATGTCTTTTg                                                                >  2:258290/1‑251 (MQ=255)
                                                                                                                                                                 ttACCAGTGCAGCGCCAGCCAACGTGCCCTTGTTAGAAAACTGAGTATTCTCAACGCTGATGTTCTAAC‑TATACGCAGGAAGGGCCTGATTACGGATGTTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACGTATCGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCTCCATGATGGCAAGAATGCGCCCGGCATGCGCGGCCACCCCGGTAATGATGTCTGTTGCCAGTATATc                                                      <  2:255874/250‑1 (MQ=255)
                                                                                                                                                                    ccAGTGCAGCGCCGGCCATCGTGCCCTGGTTAGAAAACTGAGTAACCTCAACGCTGATTTGCTAAC‑TATACGCAGGAAGGGGCTGCTTACGGATGTTGTCTTAGATGACCGGGAAGAGTATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCTCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgccg                                                  <  2:274672/251‑1 (MQ=255)
                                                                                                                                                                              gcCTGCCATCGTGCCCTGGTTAGAAAACGGAGTCCTCTCAACGCGGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATgccg                                        <  2:13925/251‑1 (MQ=255)
                                                                                                                                                                                                gTTAGAAAACTGATTACTCTCAACGCGGATGTGCTGAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGGCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCa                      <  2:98660/251‑1 (MQ=255)
                                                                                                                                                                                                        aCTGAGTACTCTCAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAAc              <  1:217491/251‑1 (MQ=255)
                                                                                                                                                                                                         cTGAGAACTCTCAACGCTGATGTGCTAGC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGAAATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAACATGATGGCAAGAATGCGCCCGCCAt                                                                                              >  1:274713/1‑170 (MQ=255)
                                                                                                                                                                                                                    cAACGCTGATGTGCTAAC‑TATACGCAGGAAGGGACTGCTGACGGATACTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGt  >  1:229970/1‑251 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
GTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGAC‑TATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGCCGCGCAATGCCGCCTGACACAATGAGTCCACGGTTAACGGTTGTCCATGT  >  NC_000913/1667233‑1667695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑