| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,652,331 | 0 | T | C | 90.9% | 62.4 / NA | 22 | intergenic (+2346/+397) | ydfD/ynfP | lysis protein/protein YnfP |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (9/11); minor base A (2/0); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG > NC_000913/1652098‑1652574 | cTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGGACCAAATGGGGT‑ATTTATCTTTTTAAGCCAACAAACGACCATCTCCGGGGGCCGGTGACAATAACTAGGCTGAGCAAAAAGCATAACCTTGTTGTTTACCTCCGAGAGttttgcaa > 2:72306/1‑243 (MQ=255) gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGGCATTCATCTTATTAAACCCGCAATACACGCTCTCCGGAGTCAGAGGACATTAACGAAACTGAGCAAAGAGCAAATCATTCATGTTTCCATCAGAGAGACTGGcagaa > 2:233493/1‑250 (MQ=255) aGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGATGACATAGATCTGAAAAATGGAACAATGATGATCAGTAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCGCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGATCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACaaa < 1:266782/251‑1 (MQ=255) ggcGCCCTGGTGAGCTGTGTGCACATGGATGGGAGTACATAGATCTGAAAAATTGAATAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACCGGCGTGAACCAA‑TCGTGTCATTCAGCTTATTAAGCCAGCAATCGACGCTCTCTGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGGTCACCTCAGAGATTATTGCAGAACAGAAAAACaaaaa < 2:221732/249‑1 (MQ=255) aaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAACAACACAAGCGGGAACCAA‑ACGGGTCATTTATATTATTAAACACCCAATAGACGCGACCCGGAGTCCGATGCCATACACGAGACTAAGCAAACAGCATATCATTGATAGTCACCGCAGAGGGTATATCAAAACAGAAAAAGAAAAAAGAGCCTTTTTTTTTCAACATGAAGTGGCAG‑CGGGA‑Taaaaagatta > 1:35388/1‑245 (MQ=255) aTGGAACAATGAAGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGTCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGTCGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGt < 2:81895/251‑1 (MQ=255) tgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGGAACAA‑TCCGGTCATTCCTCTTTTTTAGCCAGCAATCGACGCTCACCGGAGGCAGCTGAAATTAACGGGCCTGAGGCAAGAGAATATCATTAATGTTCACATCAGAGAGAAAGGCAGAAAAAAAAAACCAAAATGCACCTTTGTTTTTCGACCTGAAGTGTCAGGCGA‑AGTAAAAAAATTTTGTGACCATTTTAcc > 1:156535/1‑251 (MQ=255) agatCAGGAGAAATGTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTAGTAAGCCAGCAATCGACGCTCTCCGGAGGCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTAcc < 2:222438/250‑1 (MQ=255) gagaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCTGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACt < 2:131432/251‑1 (MQ=255) aGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAgg < 1:72306/250‑1 (MQ=255) cGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGa < 1:194713/251‑1 (MQ=255) cGGGAACCCA‑ACGGGTCATTCATCTTATTAAGCCAGCAATCGACGCGCTCCGGAGTCAGATGTCATTAACGAGACTGAGCAAAGAGCATAGCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTc < 2:34496/251‑1 (MQ=255) aaCCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAACAAAGAGCACATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTccacc > 1:128534/1‑248 (MQ=255) catCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATc > 1:57827/1‑250 (MQ=255) taAGCCAGCAAGCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCTCCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGTCTCAATAAGGCAGATGTGGTACGCTGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCgaga < 1:175019/250‑1 (MQ=255) ggAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTg < 1:233493/251‑1 (MQ=255) aTGACATTAACGAGACTGAGCAAAGAGAATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTgg > 1:265729/1‑251 (MQ=255) aGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat > 1:295507/1‑251 (MQ=255) aaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTc > 1:169164/1‑193 (MQ=255) gagCATATCATTGATGTTTCCCTCAGAGAGTATAGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACTTGAAGTGTCAG‑CGAGAGTAAAAAATTATTGTGACCACTTTACCGTTGACTCAATAAGGCAGATGGGGGACGCAGCGATAAAACGTGCCGGACTCCGCCGTCGAAAATCATATCAGTCGTGACATCCTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCaaa < 1:48813/251‑1 (MQ=255) agCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATAGAAAATCATATCAGTCGAGACATACTTATACCTGCTGGTCGCTGACAGCTGGAGCTAACCCGGCATTTATAGCAAAc > 2:214512/1‑251 (MQ=255) tGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAACAATTATGGTGACAATTTTACCGTTGACTCAATAAGGAAGATGTGGAAAGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGTGGCTAACCAGGCATTTATAGCAAACCAGATGGGCCagg > 1:192647/1‑249 (MQ=255) | CTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAA‑TCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAG‑CGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG > NC_000913/1652098‑1652574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |