Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,247	C→T	I11I (ATC→ATT)	ydfZ →	putative selenoprotein YdfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,247	0	C	T	93.8%	36.6 / NA	16	I11I (ATC→ATT)	ydfZ	putative selenoprotein YdfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/6);  minor base G (0/1);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTT  >  NC_000913/1629002‑1629470                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                ccGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCACAAAAGATGTGAGCTTGCTCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACAACTATGACGTCCTACGATCGTAACCGTAACGCAATtaccac                                                                                                                                                                                                                            >  1:184765/1‑251 (MQ=255) ccGCCAAGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACTCAATtacca                                                                                                                                                                                                                             <  1:224755/250‑1 (MQ=255)                            cGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAACGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTAGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGccc                                                                                                                                                                                                 >  2:179705/1‑249 (MQ=255)                                         agttcttatcATCCCATCCGGTGTGAGATTCTTTTCCCCCGCCTTATGGCTAATGCATGCTGCAAAAAAGATGTGAGCTAGATCAAAAACAAAAATTATTTCACTCGATAGGAGTATTTATATTGCGCCTGTTACGTTGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATGGCCACTGGCAGCCGTGTTATGGTTAGCGGCACCGTTCACACTGGc                                                                                                                                                                                    <  2:136830/243‑1 (MQ=255)                                                            ggggTGAGAGTCTGTTCCCCCTCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAATCAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACTCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATa                                                                                                                                                                 <  1:282426/251‑1 (MQ=255)                                                                                        ggCTCATGCATGCATCAAAAAAGATGTGAGCTTGATTAAAAACAAAAAATATTTCATCCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCTTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATcc                                                                                                                                      <  1:51315/250‑1 (MQ=255)                                                                                         gCTCATGCATGCATCAAAAAAGATGTGAGCTTGGTCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGCTCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACCCTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAGTccgc                                                                                                                                    >  1:181639/1‑251 (MQ=255)                                                                                                                tgtgAGTTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGCAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGGGTTATGATTAGCGGCACCGGTCTCACTGGCAAGAGCCTGTCGATTGATACTGCAGGTCTGCCCGCTGAGCAAATCCGCCGCGGAAAAACCGTGGttgttg                                                                                                              >  2:274792/1‑250 (MQ=255)                                                                                                                                       aaTATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGCCGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGcac                                                                                      >  1:289865/1‑251 (MQ=255)                                                                                                                                       aaTATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTTAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGcac                                                                                      <  2:81955/251‑1 (MQ=255)                                                                                                                                                   gACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGACGGTTGTGACGAGAAACTGGCACCACTGGACCTg                                                                           >  2:43210/1‑250 (MQ=255)                                                                                                                                                           tATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACAGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGATGAAGGTTGTGAAGAGAAACTGGCACAACTGGACCTGATTCGtctc                                                                  >  1:46162/1‑251 (MQ=255)                                                                                                                                                                                             gTAAATCAGAAAGTAGAAAACACCGATAACGACCTACGAGCGTACCCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggc                                <  2:59871/251‑1 (MQ=255)                                                                                                                                                                                                aataaGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGTCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggcggc                             <  1:173953/247‑1 (MQ=255)                                                                                                                                                                                                                acacCTATGACGACCTACGATCGTAACAGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTc             >  1:236945/1‑251 (MQ=255)                                                                                                                                                                                                                      aTGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGa        >  1:143582/1‑250 (MQ=255)                                                                                                                                                                                                                        gacgacCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAat      <  1:80882/250‑1 (MQ=255)                                                                                                                                                                                                                           gacCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATAttt  >  1:161944/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                CCGCCAGGAAAACAGCGACTGGTTTAGCGAAGAGTAATTCATTTCCTCTCATCCCATCCGGGGTGAGAGTCTTTTCCCCCGCCTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACCTATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTT  >  NC_000913/1629002‑1629470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑