Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,584,092 G→A intergenic (‑133/+115) safA ← / ← ydeP two‑component system connector SafA/putative oxidoreductase YdeP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,584,0920GA87.5% 54.9 / 2.0 25intergenic (‑133/+115)safA/ydePtwo‑component system connector SafA/putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (8/13);  total (10/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

AAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGATTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAGTTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCAACCGGAATACTTTTATAGCCAGGAATGCCACTTAATGGATCGTGGTTATCAAGTGTTAGCATGTGATTCGATTCTGGAAAATAGGTCACCAGTGAGCGGTCAGCC  >  NC_000913/1583849‑1584333
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 
aaaaaTGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGATTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATTTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAGTTTatat                                                                                                                                                                                                                                            <  2:12648/251‑1 (MQ=255)
     tGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGATTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTtat                                                                                                                                                                                                                                       >  1:282836/1‑251 (MQ=255)
                   attgtCAGTAATAATACAACCACAAGTGCATACATGAGTTCTGTATAGTGGTCGTTGTGCGTGATTTTGGTTTGCACTGGGGTATCATGGATTTCAATTATGTTTATTTAGCTGATATCTTTAAAAAGCGTTAACCAGGATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACGGGTGCGTCATTACTGAGTGGAGTTCCTTACATCTTTAGGGATACATTAATATCTTATATGATGTAGtttaa                                                                                                                                                                                                                         <  2:294004/249‑1 (MQ=255)
                        cagaaataatACAACCACAATTGCAGTCATGATGTATTTATAGTTGTCTCTTTGCGTTATTTTGTTATTCACTGTGGGAGCATGTATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACACGCTGGAGAAAAGAGGAAATAGGAATGGTGCTTCAGTACTGAGCCGAGTTTCTGATAGCTGTAGGCAGAAATTTATATCTAATAGTATGTatgtcaattta                                                                                                                                                                                                                     <  2:238410/246‑11 (MQ=255)
                                         caATTGCAGACATGAGTTCTTTATAGTTGTCTCTTTACGTTATTGTGATTTTCACTTTTGTCGCATGTAATTCAAATATGGTTACTTATCGGATAACGTTAAAAAGCGTTTATCAGGATGCTTAATATAACAGGCTGGAGAAAAGAGGATATAGTACTGGTTCTTCAGTACTGGTCTGAGTTTCTTACAGCAGTAGGCAGAAATTTATATCTTATAGTAGGTAGTTTAATTTAATCCATTTGAGCAAATTg                                                                                                                                                                                                   <  2:275247/251‑1 (MQ=255)
                                           aTTGCATACATGATTTCTTTATAGTTGTCTCATTGCGTGAATTTGTTTATCACTGTGGTCACAAGCATTTCAAATAGGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCATAAGATAACAGGATGGAGAAAAGAGGAAATAGGACAGGTGCTTCAGTACTGAGAGGAGTTTCTTACAGATGTAGGCAGAAATTTATATCTTATAGTATGTAGTGTAATTTAATCCATGTGAGAAAATTGCg                                                                                                                                                                                                 <  2:30549/251‑1 (MQ=255)
                                               cAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGATTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAaat                                                                                                                                                                                             <  1:194896/251‑1 (MQ=255)
                                                ggACATGATTTCTTTATAGTTGTCTCTTTGCGTGCTTTTGTTTTTCACTGTGTTCGCATGCATTTCAAATATGTTTATTTATCGGATAAAGTTAAAAATCGTTCATCAGTATGCTTAATATAACAGGCTGGAGAAATGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGGTTCTTACAGATGGAGGCAGAACTTTATAACATATAGTATTTATTTTAATTTAATACATTTGAGCAAATTGAGAaat                                                                                                                                                                                             >  2:120815/2‑250 (MQ=255)
                                                          tCTTTATAGTTGTTTCTTTTCGTGATTTTGTTTTTCACTGTTGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAAt                                                                                                                                                                                   <  2:234620/250‑1 (MQ=255)
                                                               ataGTTGTCTCTTTGCGTGATTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGa                                                                                                                                                                              <  1:102928/250‑1 (MQ=255)
                                                                         ctTTGCGTGATTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTctgct                                                                                                                                                                   >  1:152000/1‑251 (MQ=255)
                                                                                                   gagctcatgcatTTCAAATATGTTTATTTAGTGGATAACGTTAAAAATCGTTAATCAGTATGCTGAATATAACAGTCTGGAGAAAAGAGGAATTATGATGGGTGCTTTAGTACTGAGCGGATTTTCTTACAGCTGTAGGCAGAAACTTATATCTTATAGTATTTAGTTTAATTTAATCAAGGTGAGCAAATTGCGAAATTATAGTCAATGAGGAATTCTTCTGCTGTACGGATGACAGCAGAAGAACTga                                                                                                                                          <  2:197930/245‑1 (MQ=255)
                                                                                                            catTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTATTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAATTTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAGGAAATGAGAAGAGGCAt                                                                                                                                >  2:85516/1‑251 (MQ=255)
                                                                                                                                  cGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAATAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCaa                                                                                                          <  1:288977/251‑1 (MQ=255)
                                                                                                                                       accGTTAAAAATTGTTAATTAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAAGAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTGCAGCTGTAGTCAGAAAGTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCGTCTGCTGTGCGGATGACATCAGAAGAAATGAGAAGAGGCATTAATTTTATGGTTCTAATTCAACCGGa                                                                                                     <  2:276960/249‑1 (MQ=255)
                                                                                                                                       aaCGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAACTTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCAACCgg                                                                                                      >  1:6479/1‑250 (MQ=255)
                                                                                                                                          gTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCAACCGGAATa                                                                                                  <  1:215504/251‑1 (MQ=255)
                                                                                                                                           ttAAAAATCGTTAATCCGTATGCTTAGTATAACAGGCTGGAGCCAAGAGGAAATAGGAATGGTGCTTCAGTACTTAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCAACCGGAATAc                                                                                                 >  2:183300/1‑251 (MQ=255)
                                                                                                                                                                  ttAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAGTTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAAttct                                                                                                                                                                         >  2:122884/1‑156 (MQ=255)
                                                                                                                                                                  ttAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAGTTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAAttct                                                                                                                                                                         <  1:122884/156‑1 (MQ=255)
                                                                                                                                                                           cAGGCTGGATAAAAGAGGAAATTGGACTTGTGCTTCAGTACTTAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAAGGAGGAATTCTTCTGCTGGGCGGATGACAGCAGAAGAAATGAGAAGATGCATTAATTTGATTGCTCTAAGTCAACCGGAATACTTTTATAGCCAGTAATGCCACTTAAGGGATCg                                                                 <  2:21366/251‑1 (MQ=255)
                                                                                                                                                                             ggCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGGATGTAGTTTAATTTAATCCATGTTAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCAACCGGAATACTTTTATAGCCAGGAATGCCACTTAATGGATCGTg                                                               >  1:159805/1‑251 (MQ=255)
                                                                                                                                                                                                tAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCAACCGGAATACTTTTATAGCCAGGAATGCCACTTAATGGATCGTGGTTATCAAGAGTTAGCAtg                                            >  1:181087/1‑251 (MQ=255)
                                                                                                                                                                                                                                         gTAGTCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCAACCGGAATACTTTTATAGCCAGGAATGCCACTTAATGGATCGTGGTTATCAAGTGTTAGCATGTGATTCGATTCTGGAAAATAGGTCACCAGTGAGCGGTCa     <  1:10637/249‑1 (MQ=255)
                                                                                                                                                                                                                                           aGGCAGAAATTTATATCTTATAGTATGTAGTTTAATTTAATCCAAGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCAACCGGAATACTTTTATAGCCAGGAATGCCACTTAATGGATCGTGGTTATCAAGTGTTAGCATGTGATTCGATTCTGGAAAATAGGTCACCAGTGAGCGGTAAGcc  >  1:170508/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 
AAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTATAGTTGTCTCTTTGCGTGATTTTGTTTTTCACTGTGGTCGCATGCATTTCAAATATGTTTATTTAGCGGATAACGTTAAAAATCGTTAATCAGTATGCTTAATATAACAGGCTGGAGAAAAGAGGAAATAGGACTGGTGCTTCAGTACTGAGCGGAGTTTCTTACAGCTGTAGGCAGAAGTTTATATCTTATAGTATGTAGTTTAATTTAATCCATGTGAGCAAATTGCGAAATAATAGTCAATGAGGAATTCTTCTGCTGTGCGGATGACAGCAGAAGAAATGAGAAGAGGCATTAATTTGATGGTTCTAATTCAACCGGAATACTTTTATAGCCAGGAATGCCACTTAATGGATCGTGGTTATCAAGTGTTAGCATGTGATTCGATTCTGGAAAATAGGTCACCAGTGAGCGGTCAGCC  >  NC_000913/1583849‑1584333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑