| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,441,038 | G→A | intergenic (‑254/‑20) | ydbK ← / → ydbJ | putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,441,038 | 0 | G | A | 81.0% | 43.7 / ‑1.7 | 22 | intergenic (‑254/‑20) | ydbK/ydbJ | putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (6/11); minor base T (4/0); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCG‑CCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTG‑CCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGT > NC_000913/1440836‑1441282 | cAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGc < 1:108668/251‑1 (MQ=255)cAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGc < 2:201824/251‑1 (MQ=255) cAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTTGCTTTAAGATCAGAGGAAG‑AAAA‑AAAGCGGGCAGCG‑TTTTGGGGAggggggggcgctttttttttgt > 1:273404/1‑232 (MQ=255) aTTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGAAATGAATGTTTTGATCAAACAGAGGGCAAACAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTTAAGATCAGAGGAAGGAAAA‑G‑TGCGGGCAACG‑CTTTTGGTAGGGTGTGCCGCTTTAttgtttt > 1:192977/1‑247 (MQ=255) aGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCAATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGGTTGGCTTAAAGATCAGAGGAAG‑AAAAAGA‑GCGAGCCACG‑GTTTGGGGGGGGGGTGCCGCTTTATTTTTTTCGGCgtg > 1:196900/1‑251 (MQ=255) ttcttcTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGaa < 2:273404/250‑1 (MQ=255) tGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGtt < 2:91785/250‑1 (MQ=255) aTTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTTAAGATCAGAGGGAG‑AAAA‑GATGCGAGGAGCG‑GTTTGGGTAGGGGGTGGCGCTTTTTTGTTGTCGGGGGGTGAG‑AGTGAACCTGTTtagc > 1:252536/1‑251 (MQ=255) tgtgTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGc < 1:291803/250‑1 (MQ=255) aatgaatgTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGcc < 1:17986/231‑1 (MQ=255) tgaatgTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTTTTTTCGGCGGGT‑AGTAGTGAGCCTGGTTAGTAGGCGACTGCCGCGCACGCAGCG‑CCAGGtttt > 1:37285/1‑249 (MQ=255) aatgTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCG‑CCAGGTTTaaaa < 1:897/251‑1 (MQ=255) aaaTAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑AATGCGAGCAGCG‑TTTTGGGGAGGGGGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGGGGACCTGTTCAGCAGGCGGCTGCCGCGCACGTAGCG‑CCAGGTTTAAAAGCGTCGATGTCCCGTAGTGGGGAAgg > 1:224363/1‑249 (MQ=255) aaTAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCGCTCTGTGCTGCCTATTATGTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAT‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTGGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGCGGCGCACGTAGCG‑CCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCaa < 1:139046/251‑1 (MQ=255) gCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCG‑CCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATtg < 1:279534/251‑1 (MQ=255) accATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTTCCGCTTTATTGTTGTCGTCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCG‑CCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATtg < 1:266121/250‑1 (MQ=255) gCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTTAAGATCAGAGGAAG‑AAAA‑GATGCGGGGAGCG‑TTTTGGGGAGGGGGTGCCGCTTTATTGTTTTCGGCGTGG‑AGTAGTGAAACTGGTTAGCAGGCGACTGCCGCGCACGGAGCG‑CCCGGGTTAAAAAAGTCGGAGTTCCGTAGAGGGGAAGGAAAATGTGCAATGagtggcgggtcgggttt > 1:287757/1‑235 (MQ=255) gCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAGG‑AAAA‑GATGCGGGCAGCGGTTTTGGG‑AGGGGGTGCCGCTTTATTGTTGTCGGGGGGG‑AGTAGGGAACCTGTTCAGCAGGCGGCTGCCGCGCACGGAGCGGCCGGGTTAAAAAGCG‑CGGTGGTCAGGAGGGGGGGAGCAAATTTTGCAATTATCGGCGGGTTGCtttt > 1:112962/1‑250 (MQ=255) gctgcCTATTATTTATGGCGGTGTCGTTTGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCG‑CCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTG‑CCCGTCAACTGGATGGTACGGCGATTGGGAt < 1:74533/251‑1 (MQ=255) tttGGCTTAAAGATCAGCGGAAG‑CAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGCGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGCACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCG‑CAAGGTTTAAAAGAGTCGTTGTCCAGTAGTGGAGAAGCAAATTGTGCAATGCTCGGCGGTTCGCTTTCTTTTGACCTGTCAGTTGG‑TGGTACGGGCATTCGGTTGTTTGCATTACCCAACgtcacaaccc > 1:203953/1‑242 (MQ=255) tttGGCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCG‑CCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTTTTTTG‑CCCGTCAACTGGGTGGGACGGCGGTTTGGGTGTGTGACTTACCCAAcagccaaagcg > 1:42696/1‑241 (MQ=255) ggCTTAAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCG‑CCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTG‑CCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGt < 1:153576/250‑1 (MQ=255) | CAGTAGTAATCTTTCAATTTTAGCAAATGGCTTTCTTCTGCATTTTCGCTTTTGTGTCCCCCACATCAGCGTAATGAATGTTTTGATCAAACAGAGGGCAAAAAAATAGCCATAAAAAAGTAAAAATGCGGGGCAACGCGCAGGACAGCTCTCGACGCCGCCCTCTGTGCTGCCTATTATTTATGGCGGTGTCGTTTGGCTTGAAGATCAGAGGAAG‑AAAA‑GATGCGAGCAGCG‑TTTTGGGTAGGGTGTGCCGCTTTATTGTTGTCGGCGTGT‑AGTAGTGAACCTGTTCAGCAGGCGACTGCCGCGCACGTAGCG‑CCAGGTTTAAAAGCGTCGATGTCCAGTAGTGGAGAAGCAAATTGTGCAATGATCGGCGGTTCGCTTTCTGTTG‑CCCGTCAACTGGATGGTACGGCGATTGGGATGTGTGCATTACCCAACGGCAAACGCTGT > NC_000913/1440836‑1441282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |