Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,358,8590TC92.9% 34.7 / NA 14Y110C (TAT→TGT) puuPputrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (8/5);  minor base A (1/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAAC‑ACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAA‑TCCAGCAGCGATGACCAGCCG‑ACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGT‑AGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGC  >  NC_000913/1358612‑1359091
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           
ccAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTTAACTACCAGGAAGATAGAC‑TCCACCATTATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCCCGGCGGCACTTCCGGGAAGAGTGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑t                                                                                                                                                                                                                                         <  2:197108/251‑1 (MQ=255)
                    ctcCTTTATGCAGTCCCTGAACTACCAGGAAGATAAAC‑ACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCCCCCCCACCCACGGCGGCCCCTCCGGGAAGGAGGCGGGGAGAAAGATTTTCTCCAACAAGACGTTAATCATCGGCCAAAAGAGACAA‑TACCGCAACGATGGCCAGccc                                                                                                                                                                                                                     >  1:291049/1‑250 (MQ=255)
                    acccgtgaaggagtcacagAACTACAATGAAGATAAACCACGAC‑ATGATGGAGAGTTGCAACAGTACAAACAGGGGATTGAAGTTAGCGAGCAGGTTGACTCTCTTCAGATTCGCGGCGGATAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGACTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACAAGcc                                                                                                                                                                                                                      <  2:2359/232‑1 (MQ=255)
                                       accTACCAGGAAGAGAAAC‑ACCACCAGGATGGAGATTTGTACCCGTACAACCAGGGTATTGAAGTTAGCGCCCAGGTTGACGCTCTTCAGATTCGCGTCGGTTAAAATGGCGACGAAGGTTACCTCCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACATTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCG‑ACCATAAATCCGACGTGCg                                                                                                                                                                                                 <  2:236997/249‑1 (MQ=255)
                                              aGGAAGATAAAC‑ACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCG‑ACCATACATCCGACGTGCGGGTTAAt                                                                                                                                                                                          <  2:180146/251‑1 (MQ=255)
                                                gAAGATAAAC‑ACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACGTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCG‑ACCATAAATCCGACGTGCGGGTTAATCg                                                                                                                                                                                        <  1:145/251‑1 (MQ=255)
                                                       aaC‑ACCAACATGATGGAGATTTGCACCAGTACAAACAGGGAATTGAAGTTAGCGAACAGGTTGACGATCTTCAGATTCGCGGCGGTTAAAATGGCGAAGGAGGTTACCACCCACACCAAAGGAGGCCATTCCGAGCAGAGGGCGGAGAGATAGATTTTAGCCAACAAGAAGTTAATCATCGGCACAAAAAGACAA‑TCCAGCCGCGAAGACAAAACG‑ACCATAAATCCGAAGTGAGGGGTACTAGACttttt                                                                                                                                                                                 >  1:287015/1‑250 (MQ=255)
                                                                                            aGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGGGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCCAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCG‑AACATAAATCCGACGTGCGGGGTAATCGACTTTTGCGCGT‑AGGTATTAGCCGCACCCGCCTCCGGAAACTg                                                                                                                                            >  1:167472/1‑251 (MQ=255)
                                                                                                  ttGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGCAGGTTACCACCCCCACCCACGGCGGCCCCTCCCGGGAGGGGGCGGGGCGAGAGATTTTCACCAACACGACGTTAATCATCGGCAAAAA‑ACAAAACACCCGCCGCGATGACCCGCCGCACC‑TAAATCCGCCGTCCGGGGTAATCACCTTTTGTGCGTCAGG‑ATAGGCCGagacctaccccgacaacagtcggac                                                                                                                                      >  2:138865/1‑227 (MQ=255)
                                                                                                                                         gcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAACAGAGACAA‑TCCAGCAGCGATGACCAGCCG‑CCCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGT‑AGGTATAGGCCAAACCGGCCTCCGGAAACTGGCGAACCCGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgc                                                                                               >  2:290901/1‑251 (MQ=255)
                                                                                                                                                                                                                gatTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAC‑TCCAGCAGCGATGACAAGCCG‑ACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGT‑AGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAAt                         >  1:236189/1‑250 (MQ=255)
                                                                                                                                                                                                                gatTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCG‑ACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGT‑AGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCacat                         >  2:227323/1‑247 (MQ=255)
                                                                                                                                                                                                                        ccAACAAGACGTTAATCATCGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCG‑ACCCTAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGT‑AGGTATAGGCCGAACCGACCTCCGGAAACTGGCGCACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCATCGCCAGCAAATAGGACGCCGGAACGTGACCGCCGCTAATGCCGGAGACAATACCAAGAGTATCAAATACGGTCATc                >  1:190723/1‑251 (MQ=255)
                                                                                                                                                                                                                                      atcatcGGCAAAAAGAGACAA‑TCCAGCAGCGATGACCAGCCG‑ACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGT‑AGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGc  >  1:239903/1‑251 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           
CCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAAC‑ACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAA‑TCCAGCAGCGATGACCAGCCG‑ACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGT‑AGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGC  >  NC_000913/1358612‑1359091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑