Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,357,2020CT92.9% 82.2 / ‑1.1 29intergenic (‑92/+221)sapA/ymjAputative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (12/14);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

GGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTG  >  NC_000913/1356973‑1357440
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              
ggTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCAAAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTATATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACAGTTTGTGTTCAGGGATCGTAATGAGGGCTGAGTGGTTGGTCGTTGAAc                                                                                                                                                                                                                             >  1:185659/1‑249 (MQ=255)
                  gACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTTAACAGGTGACGTCGCCATCTGtt                                                                                                                                                                                                         >  1:32162/1‑251 (MQ=255)
                                aaCCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTAccc                                                                                                                                                                                           >  1:126519/1‑251 (MQ=255)
                                    gCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGa                                                                                                                                                                                       <  1:243679/251‑1 (MQ=255)
                                                  cAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGTCATTTGCCATTTCCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATTTGTTCAACATTCGTACCCGTGATTTCTctataacta                                                                                                                                                                         <  1:60524/251‑1 (MQ=255)
                                                   aGCAGGCGGGGGAGATTCAGGCGCGGCGAGTGCCTGACCACTCACAAGGCCAGCAATAACCAAAAGAGCCGAAAATTCCTTGCGCATAATATTAAGGGATTTTATGTATAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCGctataactat                                                                                                                                                                        <  2:246145/251‑1 (MQ=255)
                                                      aTGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCtataactataac                                                                                                                                                                     >  1:231917/1‑251 (MQ=255)
                                                                   tCAGGCGCGACGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACCTTCGTACCCGTGGTTTCTCTATAACTATAACTCACAGAACAACt                                                                                                                                                        >  2:19814/1‑251 (MQ=255)
                                                                     aGGCGCGGCGATTGCTTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGTCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTTAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAGCTTa                                                                                                                                                      <  2:32162/251‑1 (MQ=255)
                                                                                             caaaaGTCCAGCAATCATCAAAAGAGACGATAATACCTTGCGCATAATATTAAGGTATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACGCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATc                                                                                                                               <  2:226596/247‑1 (MQ=255)
                                                                                               caAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGc                                                                                                                                        >  1:178323/1‑239 (MQ=255)
                                                                                               caAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGc                                                                                                                                        <  2:178323/239‑1 (MQ=255)
                                                                                                      aGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGa                                                                                                                                                                                       >  2:42609/1‑185 (MQ=255)
                                                                                                      aGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGa                                                                                                                                                                                       <  1:42609/185‑1 (MQ=255)
                                                                                                                aaaaGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGc                                                                                                            >  1:188818/1‑250 (MQ=255)
                                                                                                                     agaCGCTAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGt                                                                                                                                     >  2:173198/1‑220 (MQ=255)
                                                                                                                     agaCGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGt                                                                                                                                     <  1:173198/220‑1 (MQ=255)
                                                                                                                       aCGATAATCCCTGGCGCATCATATTACGGGATTTTATGTACAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGCGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGaa                                                                                                                                                                                                                              >  2:186700/1‑129 (MQ=255)
                                                                                                                         gagaataCCGGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATAACATTTGCCATTACCGTTTGTGTTCAGGTGTCGTAATATGGGCTGATAGGTTTGGCGTTGAACAAGTGAAGTCTCCATATGTACAACATTCGAACCCGTGATATATCAATAACTAAAACTAACAAAACAACATAGCGAGGAGCAGATCGGTAAGACCGTAAGAAACACATTACGGCACGCTGAAGt                                                                                                  >  2:80314/4‑251 (MQ=255)
                                                                                                                              tgaatGGCGCATAATGTAAAGGGATTTTCTGTAAAGCGGCTATCTTACTCATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGTGTCGTAATGAGGGCAGTGTGGTTGGTCGTTGACCAGGGGCCGCCGCCATTGGTTCAACATTCGTACCCATGCTTTCTCTGTAACTATAACTCTCAGAAATCCTTAGCGGGGAGCATAGCAGTACGAACGTGCGACACCCATTCAAGCACGCTGCatagcgtg                                                                                             >  2:18375/5‑244 (MQ=255)
                                                                                                                                               tAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTGTGGGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGAt                                                                             <  2:153064/250‑1 (MQ=255)
                                                                                                                                                aaGGGATTTTATTTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATg                                                                            <  2:290470/250‑1 (MQ=255)
                                                                                                                                                               aaGAGGCTATCTTCCTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCCGGGTTCGTAATGAGGGCTGTGTGATTGGTCGTTGAACAGGTGACGTCGCCATCTGTTAAACATTCGTACCCGTGTTTTCTCTATAACTATAACTCACAGAACCACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGACACCCATTAAAGCACGACGCAGAGCGTGTCTTACTTAGCCAGACGCGGCGTACACGCttt                                                             >  2:99305/1‑250 (MQ=255)
                                                                                                                                                                               aaTATTTAATGACATTTGCCAGTACCTTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTAGAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACt                                            <  1:115765/251‑1 (MQ=255)
                                                                                                                                                                                      aaTGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGa                                      <  1:207728/250‑1 (MQ=255)
                                                                                                                                                                                                        cggtggtgtgtaGGGGTCGTAATGATGGCTGTGTTGTTGGTCGTTGAATAGGTGACGTCGCCATCTGTTCAACATGCGTACCCGTGATTTCACTATAACTATAACGCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAATCACGCTGCAGGGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtcat                   <  2:236278/240‑1 (MQ=255)
                                                                                                                                                                                                        cGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtcat                   <  1:139182/251‑1 (MQ=255)
                                                                                                                                                                                                        cGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTAACCGATCGTCTTCAATTAAAtcat                   >  1:73652/1‑251 (MQ=255)
                                                                                                                                                                                                                         cGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTg  <  1:14585/251‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              
GGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATTG  >  NC_000913/1356973‑1357440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑