Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,337,3940AG94.4% 50.2 / NA 19S522G (AGC→GGC) acnAaconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base G (10/7);  minor base T (0/1);  total (10/9)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCC  >  NC_000913/1337162‑1337636
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   
gATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                                                                                                                                                                                   <  1:44993/250‑1 (MQ=255)
   ggCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtgcgg                                                                                                                                                                                                                               >  1:211016/1‑251 (MQ=255)
   ggCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtgcg                                                                                                                                                                                                                                <  1:79833/250‑1 (MQ=255)
                            gCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTg                                                                                                                                                                                                       >  1:28272/1‑250 (MQ=255)
                                                                                                             gCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAatat                                                                                                                     >  1:292256/1‑251 (MQ=255)
                                                                                                               aaaaGCGAAACTGACACCGTCTCTCGACGAACGGGGGTTTAACCTTGTGGGATACGGTTGAACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGGGCGGTGCTGTCCGGCAACCGAAAATTTGAAGGCCGTATCCATCAGCTGGTTAAAACTAACTGGCAGGACTCGCCGCAGCTGGAGGGTGACTATGAGCTGGCGGGAAATATg                                                                                                                    >  2:237864/1‑250 (MQ=255)
                                                                                                                  aGCGAAACAGACACCGTTTCTCGACGAACTGGGGTTTAACCTTGTGGCATACGCTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGCAACGGCACTCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGAAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTACCTGGCTGGCCTGGCCGCCGGTGGTGGTTGCCTATGCGCTGGCGGTCAATATGact                                                                                                                 >  2:154983/1‑248 (MQ=255)
                                                                                                                        actgaacccgtagttcgtcgaACTGGGGTTTACCCGTGTGGGATACTGTTGGACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTATCCGTCTGTGCGGGGCTGGCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTATCTGGCTGGCCTCGCCGCCGCTGGTGGTGGACTATGCGCGGGCGGGAAATATGAATATCAAcc                                                                                                          <  2:195665/233‑1 (MQ=255)
                                                                                                                         cTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATTTCAAccc                                                                                                         >  2:289850/1‑250 (MQ=255)
                                                                                                                                                  ggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGAt                                                                                <  1:216813/251‑1 (MQ=255)
                                                                                                                                                        aCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCaa                                                                           >  1:38536/1‑250 (MQ=255)
                                                                                                                                                                ggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAgg                                                                                                                                                                                                   >  2:114809/1‑122 (MQ=255)
                                                                                                                                                                ggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAgg                                                                                                                                                                                                   <  1:114809/122‑1 (MQ=255)
                                                                                                                                                                       gTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGATTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTAt                                                           >  1:242868/1‑251 (MQ=255)
                                                                                                                                                                            ccccacTGTATTGGTAACTTTGGGCCGTGGCCCGCTCGTTGCGAACGGGCAATAAAAAAAGTCGATTTAACCGTCGGTGCTGTGCTGTCCGGCATCCGTATCGTTGAAGGCTGTAGTCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTTGTGGTTGCCTATGCGCTGGTGGGAAATATGAATATCAACCTTGGTTCTGAGCCTATCGGCCATGATCGCAAAGGCTTCCCGGTTTATCTGa                                                       <  2:290929/245‑1 (MQ=255)
                                                                                                                                                                                                 gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAATGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcac                                  <  1:167579/250‑1 (MQ=255)
                                                                                                                                                                                                 gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcac                                  <  1:288079/250‑1 (MQ=255)
                                                                                                                                                                                                              aTCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGGAATATGAATATCCACCTGGCTTCTTAGCCTATCGGCCATGATTGCAAAGGCGGTCCGGTTTATCTGGAAGATATCTTGCCATCGGCACAAGACATTTCCCGt                    >  1:122376/1‑251 (MQ=255)
                                                                                                                                                                                                                            gCAACCAAAAAAGGCGATTAAACCGGCGGTGCGGTGCTGTCCGGCAACCGTACCTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGt      <  2:38536/251‑1 (MQ=255)
                                                                                                                                                                                                                                ataaaaaagGCGATGTAGCCGTCGGTGCAGTGTGGGCCGGCAACCGGAATTTTGAAGGCTTTTTCCTTCCGCGGGTTAAAACTTCCGGGCGGGCCTCGCCGACGCTTGTGTTTGTCTTTGCGCTGGCGGGAAATATGAATAGCAGCCTGGGTTCTGAGCCTACCGGCCATGATCGCAAAGACGATCCGGTTTATCTGAGAGATATCTGGCCATCGGCACAGGAAATTGCCCGTGCGGTAGAACAAGTCTcc  <  2:216820/249‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   
GATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCC  >  NC_000913/1337162‑1337636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: