Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	88.6%	81.3 / 0.3	37	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base T (21/10);  total (25/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCC  >  NC_000913/1301750‑1302215                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                cTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGc                                                                                                                                                                                                                         >  1:44707/1‑251 (MQ=255)    cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCAt                                                                                                                                                                                                                       <  2:148576/250‑1 (MQ=255)                        aTCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGa                                                                                                                                                                                                   >  1:295577/1‑250 (MQ=255)                                   cGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaaaa                                                                                                                                                                                         >  1:53931/1‑249 (MQ=255)                                      cGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGACTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAAAGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGAGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGGTCAAGAAGATGAAAAAag                                                                                                                                                                                       >  2:161371/1‑248 (MQ=255)                                                            aaTATCGTCACCCACGGTGCCTATACCTTAAAAGATTGGGACGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAAACGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGa                                                                                                                                                              <  2:89815/251‑1 (MQ=255)                                                             atatCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGAt                                                                                                                                                             >  1:64616/1‑251 (MQ=255)                                                                    cACCCACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGCATCGTTCTTTAACGCATCCCGACCTACAGGAACAACACGAAAACCATTATTAACCAGGTAACCTAGTTGCCTATTGCTTATGAA‑GTTACCGATGTAAACCGCT‑ACCGTAGTGGTGCAATCAACATTACATATAACAGCAAGCCGATCGAATTGTTCCATAAGCTGAAAAAAGAGATCCCAGACGAAGTTAACGTTGATCCATAcc                                                                                                                                                      >  2:286375/1‑251 (MQ=255)                                                                          cGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGc                                                                                                                                                 >  1:135379/1‑250 (MQ=255)                                                                           ggTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                               >  1:163869/1‑251 (MQ=255)                                                                               ccTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑AACGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGCATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTAc                                                                                                                                           >  2:121640/1‑251 (MQ=255)                                                                                       ttAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTCTTACg                                                                                                                                    >  1:206975/1‑250 (MQ=255)                                                                                          aaaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAt                                                                                                                                >  1:90813/1‑251 (MQ=255)                                                                                                     cGTAAACGAACCAATCGTTCTTTAACGCAGCCCTACCTACTGGAACTACGCGAAAACCGTTATTAACCAGTTAACCTAATTGCCTATTGCTACTGAA‑GCTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGACCGAATTGTTCCAGAAGCTGAAAAAATAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTTACTATTACGAAATTAACAACCAGa                                                                                                                     <  2:294669/251‑1 (MQ=255)                                                                                                                gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTc                                                                                                          >  1:105839/1‑251 (MQ=255)                                                                                                                  acctttCTTGAACGCAGCCAGACCTTCTGGACCAACGCAAAAACCGTTATAAAACTTGTAACCTTTTTGCCTATTGCCTCTGAATGTT‑CCAATTTCAACC‑CTCACCGTATTGGTGAAATCGCAAGGACTTATAACAGCCTGCCGATCTAATTGTTCCAGAAGCTGAAAAAAAATATCCCGGACAAATTCCCCGTGTATCCATCCCTGTGTTCTTTCTATAACGAAATTAACAACCAGAAACCGCCATTCaa                                                                                                        <  2:174310/247‑1 (MQ=255)                                                                                                                       ttgtgacaccagcctcaCCTACTGAAACAACGCAAAAACCGTTATTAACATGGTAACCTATTTGCTTATTGCTTCTAAA‑GTTACCGATTACAACCCCT‑ACACTAGTGTTTAAACCGAAATCACGTAGAACAGCATGCCGATCGAATTGGTCCAAAAGCTGAAAAAATAGATCCCTGACGAAGTTCACGTTGATCCATACCTGTGCACTTTCTATTTAGAAATTAACAACCAGAAACCGCCATTCAACGAt                                                                                                    <  2:105889/234‑1 (MQ=255)                                                                                                                       tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCTTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAt                                                                                                    <  1:161371/250‑1 (MQ=255)                                                                                                                       tCTTAAACGCAGCCCGACCTACTGGCACAACGCGAAAACCGCTATTAACCAGGTAACCTCTTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGAGGTGAAATAGTCATGACGTATAACAGCATGCAGCACGAATTGATCCAGAAGCTGAAAAAAGAGATCACAACCGAAGATCACGTTGATCCATACATGTGCAATTACTATAACGAAAATAAAAACAAAAAACCGCCATGCAACGa                                                                                                     >  2:266617/1‑249 (MQ=255)                                                                                                                                    ccGACCTACTGGAAAAACGCGAAACCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGCCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTAc                                                                                      <  1:80622/251‑1 (MQ=255)                                                                                                                                      gACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGACGCTGAAAAAAGAGATCCCGGACGAAAGTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCg                                                                                    >  2:220553/1‑251 (MQ=255)                                                                                                                                           aCTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTATTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAGTAACAACCAGAAGCCGCCATTCAACGATGTGCGTGTGCGTACCGCAc                                                                                 <  1:121640/249‑1 (MQ=255)                                                                                                                                                   aacGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAgg                                                                       >  1:206289/1‑251 (MQ=255)                                                                                                                                                    acccaaaaaCCTTTATTAACCAGGTAACCTTTTTGCTTATTGCTTCTGAA‑GTGCCCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGGATAACAGCAGGTCGAGCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCCCTTTGATCCATCCCTGTGCACTTCCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTa                                                                         <  2:33633/243‑1 (MQ=255)                                                                                                                                                    acacgAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGGATAACAGCATGTCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTa                                                                         >  1:33633/4‑248 (MQ=255)                                                                                                                                                              cGTTATTAACCAGATAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGAATGGAccacg                                                            >  1:238126/1‑248 (MQ=255)                                                                                                                                                                            tAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaat                                              >  1:145869/1‑251 (MQ=255)                                                                                                                                                                            tAACATATTTGCATATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGAATAACCGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGCTCCATACCTGTGCACTTAATCTTACGAAAATACCAACCAGAAACCGACATTCACAGATGTGCGTGGGCGTACAGCACTGAAACTAGGAATAGACTGCGCTATCATTGTtaat                                              >  2:146169/1‑251 (MQ=255)                                                                                                                                                                                    ttGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTa                                                                     >  2:226523/1‑220 (MQ=255)                                                                                                                                                                                    ttGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTa                                                                     <  1:226523/220‑1 (MQ=255)                                                                                                                                                                                     tGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGaa                                      >  1:164682/1‑250 (MQ=255)                                                                                                                                                                                             gattCTGAA‑GGTACCTACGTCAACCGCT‑ACCGTAGTGGTGAAAGCAACATTACGTATAACAGCAGGCAGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAgg                              <  2:87143/248‑1 (MQ=255)                                                                                                                                                                                               ttCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGc                            >  1:131227/1‑250 (MQ=255)                                                                                                                                                                                                        ttACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGAATGGACCGCGATATAATTGTTAATAAAGTGAAAGCGCAGGGCAACATGccc                   >  1:212410/1‑251 (MQ=255)                                                                                                                                                                                                         tACCGATGTAACCCGTA‑ACCGTATGGGTGAAATCAACAGGACTAATAACAGATTGCCGTACTAATTGTTCCAGAAGCTGAAAAAAGAGAACCCGGACGAATTTACCGTTAATCCATACCTGGGCTCTTACTATTTCGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAGTGTTAATAAAGTGAAAGCGCAGGGCAACATGccc                   <  2:50563/250‑1 (MQ=255)                                                                                                                                                                                                                       gCT‑ACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACt    >  2:83676/1‑251 (MQ=255)                                                                                                                                                                                                                           aCCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTcc  >  1:106108/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                CTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAA‑GTTACCGATGTCAACCGCT‑ACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCC  >  NC_000913/1301750‑1302215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑