| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,253,002 | C→T | intergenic (+17/+83) | dhaR → / ← ycgV | DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,253,002 | 0 | C | T | 89.5% | 42.1 / ‑0.9 | 19 | intergenic (+17/+83) | dhaR/ycgV | DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (9/8); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01 | |||||||||||
GGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCT‑GTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACTTCT > NC_000913/1252760‑1253239 | ggAACATCTGTTTCCCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAATAGTCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACAt < 1:197891/251‑1 (MQ=255) gCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTAAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACAAGGCATTTATGCAGGGCAGTTTAAACGCCGGGGATGAAAGACAGAAAAGATTTTTGTTAAATAAGAGGGATAT‑GTATTTCATTCCGGCGCACGCTAAACATTTTTTGCATAGGTTAAGGGCTTGTCTATccc > 1:47885/1‑251 (MQ=255) gAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATTTCGGCTTTACTTGGGATCGGCCGCGCTACGCTGTGGCGGGAAATGAAGCAACATGGCATTGGTTCAGGGGAGGTTAAGCGCCGGGGGTGAAAGACAGAAAAGATTTTTTATAAATCCGAGTGATCT‑GTATTTCATTTCGGGGCACGCTTACAATTTTTAGCATCGttttagaggttgtatctcccccc > 2:63424/1‑231 (MQ=255) gTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCCTTCAGGAAATGTCGGCTTTTCTTGGGGTCGGCCGCACTACGCTGTGGCGGGAAATGGAGCAAAATGGCATTTATGCAGGGCAGATTAAACGCCGGGTATGAAAGACCGAAACGATTTTTTATACATCAGAGGGATCT‑TTATTTTATTCCCGCGCACGCTAACAATTTTTAGCATCGTTTTAAGGCATGTCTATCCCGCCCtt > 1:288651/1‑251 (MQ=255) tGAAAAAGAGGCAATTAGTAACGCAGCCCAGGTCACAGGCGGTCGCAGTCAGGAAATGTCGGCTGGACTTGGGATCGGCCGCACTACGCTGTTGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCATTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGAt < 1:137665/182‑1 (MQ=255) ttCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAATCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCT‑GTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGat > 1:88192/1‑217 (MQ=255) ttCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAATCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCT‑GTATTTCATTCCGGCGCACGCTAACAATTGTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGat < 2:88192/217‑1 (MQ=255) tGTCGGCTTTACTTGGGATCGACCGCACTAAGCTGTGGCGGGAAATGAAGCCACATGGCAATGCTGCAGGGCAGTTTAAACGCCGGGGATGAAAGAAAGAAACGATTTTTGATACAACCAAGTGATCT‑GTATTTCATTCCGGCGCACGCTAACCATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTTCAGCGATATCCTCCGTTGACTTGCTTCTGATCAACGAGGTTAACCTGccc > 2:194761/1‑249 (MQ=255) cGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCT‑GTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTa < 1:63424/251‑1 (MQ=255) gggATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCT‑GTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAACGAGGTTACCCTGCGTGTAATCCGCTTCg > 1:277699/1‑250 (MQ=255) ggATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCT‑GTATTTCATTCAGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGCTCAAAGAGGTTACCCTTCGTGTAATCCGCTTCg > 2:74745/1‑249 (MQ=255) gAAAATGAAGCACCATGGCAGTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCT‑GTATTTCAGTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACATAAAAGCTGAAGCGATATCCTCCGGTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATAc < 2:101202/251‑1 (MQ=255) aGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCT‑GTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACt < 1:261664/251‑1 (MQ=255) tAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCT‑GTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCAtt > 1:248706/1‑250 (MQ=255) aaGCGCCGGGTATGAAAGCCATAAACTATTTTTAATCCATCAGAGTAATCT‑GTATTTCATTCCGGCGCCCGATAACAATTTCCAGCATAGTTTAAGGGCTTGCCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTATGGATCAAAGAGGTTACCCTGCGTTTAATCCGCTTCGAGATAGAATGTGTGCTTTTTGTTATACTGTGCCCTGACTCCCACGCCGTTATTCCAGCCATTa < 2:233898/250‑1 (MQ=255) aTGAAAGACAGAGACGATTTTTGATACATCAGAGTGATCT‑GTATTTCATTCCGACGCACGCTAACAATTTTCCGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAACGCTGAATCGATATCCTCCGTTGACTTGCTTCTGATCAACGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTAATACTGTGCACTGCCTCCCACGCCGTTATTCCAGCCATCACCTTTGAAACTg > 2:252334/1‑251 (MQ=255) ggaaGACAGAAACGATTGTGGATACAACTGAGTGATCT‑GGATTTCATTCCGGCGCACGCTAACAATTTTCAGCAGCGTTTAAGGGCTTGTCTATCCCGCACTGAAAAGCGGAAGCGATATCCGCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCGGCGTGTACTCCGCTTCTAGATAGAAGGTGTGGTGTTTGTTATATTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTa < 2:142780/249‑1 (MQ=255) acaaaaaCGATTTTTGATACATCAGAGTGATCTTGT‑TTCCATTCCGGCGCACGCAAACAATTTTCAGCATCGTTAAAGGTCTTGTCTATCCCGAACTTAAAAGCTGAAGCGATATCCTCCGTTGACGTGCTTCTGATCAAAGAGGTTACCCGCCGTGTAATCCTCTTCGATATAGATTGTGTGCTGTTTTTTATACTGTGCCCTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACTtct < 2:253167/247‑1 (MQ=255) aCAGAAGCGATTTTTGATACATCAGAGTGTTCT‑GTATTTCATTCCGGCGAACGCTAACACTTTTCAGCATAGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGATTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCCCTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTATACTtct > 2:45810/1‑251 (MQ=255) | GGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCT‑GTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACTTCT > NC_000913/1252760‑1253239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |