Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,194,922	G→A	T10T (ACC→ACT)	rluE ←	23S rRNA pseudouridine(2457) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,194,922	0	G	A	100.0%	31.0 / NA	12	T10T (ACC→ACT)	rluE	23S rRNA pseudouridine(2457) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/8);  total (4/8)

CACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGG  >  NC_000913/1194691‑1195147                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  cACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTTGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTGTCAGAGATTATGa                                                                                                                                                                                                                <  2:192903/251‑1 (MQ=255)           ccTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCAt                                                                                                                                                                                                      >  2:293227/1‑251 (MQ=255)                                      gctgcATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCGGCATTATAGCCTAATAACGCGCATCTTTc                                                                                                                                                                             <  1:236226/249‑1 (MQ=255)                                             aaaCACCCTGTACCGGGATGAAGTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTTCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCa                                                                                                                                                                    <  2:252199/251‑1 (MQ=255)                                                            ggATGAATTCTTATAATGTTTTGCGTCCGGTTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGgtagta                                                                                                                                                     <  2:49293/251‑1 (MQ=255)                                                              aTGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGAGCTGGTTTTCAGGTTTACGCCTGGTAGCACGTTGCGGGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTACTAACGCGCATCTTTCATGCCGGCAAACAATAGGGTAGTAtt                                                                                                                                                   >  2:267097/1‑251 (MQ=255)                                                                            tGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGCGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTa                                                                                                                                      >  2:112806/1‑250 (MQ=255)                                                                                       cGGCATCATCGGTGAACCGCGGAATAACATCGGAGGGTTGATGGAACCGGATCTAACGCTTGCGCCGGTTTACAGGTTTACGCCTGGTAGAACGTTGCGCGCTGAATCGCCTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATATTATTGTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGTCGGCAAACAATAGGGTAGTAATGCCAAGCCAATTACAAATCATTAAc                                                                                                                          <  2:100080/251‑1 (MQ=255)                                                                                        ggctttagctgTGAACTGTGGAAGAACATCGTTGGGTTTATTGAACAGGAGCACACGCGTGGTCGTGTGTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTGTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCAAGACGGTAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACa                                                                                                                         <  2:67884/241‑1 (MQ=255)                                                                                                                                                      gCTGGTTTTTAGGTTTACGACTAGTATATCTTTGCGAGCTGAATCGCTTAGTCTGGTGATTTCTAAAAGAATTTGTTTGCAGAGTATATTCATAGATTATTAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAAAAATAGGGTAGTATTTACAATCAAATTACAAATCATTAACAAAAATTTGCTCGAAAGCATCCGTTTCGCAGAACGCAAACGCTTAGGCATCGTGGTGGCAGAc                                                           <  2:16080/251‑1 (MQ=255)                                                                                                                                                                                                             gTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTcc    <  1:112806/251‑1 (MQ=255)                                                                                                                                                                                                               gATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACACTAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCgg  >  1:220166/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  CACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGG  >  NC_000913/1194691‑1195147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑