Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	91.4 / NA	30	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/20);  total (10/20)

TAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCG  >  NC_000913/4636678‑4637169                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     taGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCCTGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCcccc                                                                                                                                                                                                                                                   >  1:187732/1‑251 (MQ=255)  aGCCTGGGGGGCCTGTTACGCGGTTCGGCATGCTGTTGTTGCTGGGCTATTTTTTTCTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACCCCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCg                                                                                                                                                                                                                                                  <  2:57983/251‑1 (MQ=255)       gAGGTGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTcg                                                                                                                                                                                                                                             <  2:79230/251‑1 (MQ=255)       gAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGATGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTcg                                                                                                                                                                                                                                             <  1:295223/251‑1 (MQ=255)                     gCGTTTCGGCATGCGGTTGTTGCTGGGCTATTTTTTCCTGGTGGCGGTGGC‑AGCCTTGTTCGTACTGGCCATGTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCGGTCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCTCGCCAATATCGGt                                                                                                                                                                                                                                <  2:267238/250‑1 (MQ=255)                           gggaaTGCGGTTGTTTCTGTGTTATTTTTTTCTGGTGGCGGTGGC‑AGCCTGGTTCTGACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGTCGGTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCTGTTGCATTa                                                                                                                                                                                                                         <  2:50055/247‑1 (MQ=255)                                 gCGGTGGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACaaa                                                                                                                                                                                                                    <  1:122039/250‑1 (MQ=255)                                    ggttttgcgggtcgtttttgttcTGGTGGCG‑TGGCCAGCCTGGTTAGGACTGGCCATTTTTGTCAAAGAAGTTAATCCGGGCGGGCGAAGAGCAGCGGATGGGACGTTGACCGACTCCGAAATGTGGCTTGCGGAGCTGGCGGGTCCCGATTTTCCCTCTGGGGACCCAACGCATGGGCAACTGGCGCATGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATACCGGTGGCATTAACAAAGTgc                                                                                                                                                                                                                <  2:254759/229‑1 (MQ=255)                                     ttgttgCTGGGCTATTTTTTACTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCACAATCGGTGGCATTAACAACGTggg                                                                                                                                                                                                               >  1:216611/1‑249 (MQ=255)                                      tgttgCTGGGCTATTTTTTACTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTgcg                                                                                                                                                                                                               <  1:177943/250‑1 (MQ=255)                                                  aTTTTTTACTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATc                                                                                                                                                                                                    <  1:255065/249‑1 (MQ=255)                                                  aTTTGTTACTGGTGGCGTTGGC‑AGCCTGGTTCTTACTGGCCATTTTTGTCAAAGAAGGTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCTCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCa                                                                                                                                                                                                   <  1:3334/250‑1 (MQ=255)                                                    ggtgctcttccgagcttggc‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGt                                                                                                                                                                                                <  1:68495/235‑1 (MQ=255)                                                                    tggc‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCACCGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACGGGCGCAGGCGTTTAATCCGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCCATGAATATCATGt                                                                                                                                                                                                >  2:68495/1‑235 (MQ=255)                                                                           ccgggTTCGGACTGGCCATTTTTGGCAAAGAAGTTAACCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGc                                                                                                                                                                          <  2:103949/248‑1 (MQ=255)                                                                            cTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGc                                                                                                                                                                          >  1:169981/1‑250 (MQ=255)                                                                                       tGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGCGCGACGCGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTTGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAAACGGCGCAGGCGTTTAATCAACTAAAACATCCCCCGTTTCGCGACACTATCGGTGGCATTAACCACGCGAGCAATGAATATCATGTCTATATGACCGATACGCAGGGCAACGAATTGTTc                                                                                                                                                              >  2:277490/1‑251 (MQ=255)                                                                                               tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCgg                                                                                                                                                       <  1:256499/250‑1 (MQ=255)                                                                                                           gTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCAATTCGGCAAATAAAGCCGt                                                                                                                                          >  1:186788/1‑251 (MQ=255)                                                                                                             tAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGtt                                                                                                                                         <  1:22427/250‑1 (MQ=255)                                                                                                                aCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGAc                                                                                                                                     <  1:247541/251‑1 (MQ=255)                                                                                                                        tGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGCCGGAGCTGGCCCGTCCCGATTTGCTATCTGGGGACCCAACGAATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACAACCACCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGGTGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGattat                                                                                                                             >  1:129479/1‑251 (MQ=255)                                                                                                                          cGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTc                                                                                                                           <  2:104398/251‑1 (MQ=255)                                                                                                                                    cGGAGGGGATGTTGATCGACGCCTCAACGTTGCTTGCGGAGCTGGCGCGGCCCGATTTGCTCTCTGTGGACCCAACGCATGGGCAACCGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAAGATCACGTCTATATGACCGATGCGCAGGGCAAAGTATTGTGCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAAt                                                                                                                 <  2:265043/251‑1 (MQ=255)                                                                                                                                              gTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCGGGAATGACGTCTGGc                                                                                                       >  1:182426/1‑251 (MQ=255)                                                                                                                                                     gACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGGATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGtt                                                                                                >  1:56187/1‑251 (MQ=255)                                                                                                                                                                                                      gggACCCAACGCATGGGCAACTGGGGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCTCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCTGAATCCTGCCGATccc                                               <  2:187732/251‑1 (MQ=255)                                                                                                                                                                                                                               gcAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATTTCTATATGTCCGATGCGCAGGGCAAATGATGGTTCGATTCGGCAAATAAAGCCGGTGGACAGGATTATTCGCGCTGGAATGTCGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAATTTCTGTGATGGATGGTGCCg                      <  2:20093/251‑1 (MQ=255)                                                                                                                                                                                                                                           acAGCTCCAACATCCCCCGTTTCGCGACAAGATCGGTGGCATTAACAAAGTGCGAAATGAATATCATGTCGATAAGACCGAGTCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGCCAGGATTATGCGCGCTGGAATGACGGCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTGGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATgg          <  2:285508/250‑1 (MQ=255)                                                                                                                                                                                                                                                tACAACATCCCCCGTTACGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCATCACGTTGTAAAATCCTGCCGATCCAGAAAGTTCTGGGATGTATGTTGCCACACCGTATATGGACGGc     >  2:11001/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aacaTCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTcg  >  1:218918/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     TAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGC‑AGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCG  >  NC_000913/4636678‑4637169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑