Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,610	G→A	L336L (CTG→CTA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,610	0	G	A	85.7%	59.5 / 4.6	28	L336L (CTG→CTA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (11/13);  total (12/16)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

ACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTT  >  NC_000913/4620393‑4620845                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            aCGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGTCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCGCACCGCt                                                                                                                                                                                                               <  2:85324/248‑1 (MQ=255)     cAACTTCGGTAGCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACTCCACCATCAAAGAGATGAAAGAATCGTGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTTTGGCCACCGTCGCTACGTCGCCGGTTATGCCGCGGTTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACg                                                                                                                                                                                                        <  1:199495/251‑1 (MQ=255)                           gTGAAAGCGACTGGCCTGGACGCGTTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CAGCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCgg                                                                                                                                                                                  <  1:275208/251‑1 (MQ=255)                             gAAAGCGACAGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGGGATAAAAC‑CACAGTCTTCACCAACTTCGTTGACTTCGACTCATCCTGGAGACAACGTCGCGACGACGCCGGTTATGCCGCGGGAATGGAACTGATAGACCGACATCTGCCGGAACTGATGTCTCTGCTACGCGATGAAGACATCCTAATCCTCACCGCTGAACACGGTTGCGATCCAACCCGGACCGGtt                                                                                                                                                                                >  2:215205/1‑250 (MQ=255)                              aaaGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTAc                                                                                                                                                                               <  1:273468/251‑1 (MQ=255)                                       ggCCTGGACGCGCTGGTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACcac                                                                                                                                                                        <  1:59161/249‑1 (MQ=255)                                          cgtgactcgcTGTTTGCCGCCCCCATCAAAGAAATGAAAGTAACGTGGGAGTACAC‑CATCGTCTTCACCACCTGCGTTGACTTCGACTCTTCCTGGGGCCACCTTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCTCGGTTGCGATCCGACCTGGACCGGTACTGACCACAcgcg                                                                                                                                                                   <  1:273597/244‑1 (MQ=255)                                          cgggACGCGCTGATTGACGCCCCCATCTAAGATATGAAAGAAGCGTGGGATAACAC‑CATCGTCTTCCACAACTTCGTTGACTGCGACTCTTCCTGGGGCCACTGGCTCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTCCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACAcgcg                                                                                                                                                                   <  2:209783/249‑1 (MQ=255)                                          cTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACAcgcg                                                                                                                                                                   >  1:79360/1‑251 (MQ=255)                                              aCGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGa                                                                                                                                                                >  1:138191/1‑250 (MQ=255)                                                         gACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGCCATCATGATCCTCACCGCTGACAACGGTTGCGATCCGACCTGGACCGATACTGCCCACACGCGAGAACACATTCCgg                                                                                                                                                     >  2:295027/1‑250 (MQ=255)                                                          cagCCACCATAAAATGGATGAAAGAGACGGTTGATAACACACA‑CTTCTTCACCAACTTTGTTGACTTCGACTCTCCCTGGGGCCCCCGTCGCTACGTCGCCGGTTAAGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGCAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGTCCGGTACTGACCACACGCGTGAACACATGCCgttc                                                                                                                                                   <  2:59118/249‑4 (MQ=255)                                                                ccaTCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGt                                                                                                                                              >  1:131421/1‑250 (MQ=255)                                                                         agaTGAAAGAAGCGGGTGCTAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACCTCCTGATCCTCACCGCTGACCACGGTGGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGATACTGGTCTATGGcccc                                                                                                                                    >  1:151852/1‑250 (MQ=255)                                                                                        gTGATAACAC‑CATCGTCTTCACCAACTTCGTTGCCTTCGCCTCTTCCTGGGGCCACCGTCGCGACGTCGCCTGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGATCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGc                                                                                                                     <  1:166088/251‑1 (MQ=255)                                                                                         tGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAaca                                                                                                                                                             >  1:256057/1‑210 (MQ=255)                                                                                           aTAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGCTCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACCTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                                                                                                   >  2:22489/1‑250 (MQ=255)                                                                                                                 aCTTCGTTGACTTCGGCTCTTCCTGGGGCCACCGGCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACTCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTc                                                                                             <  1:27194/251‑1 (MQ=255)                                                                                                                                               cccGTCGCGACGTCGCCGGTTATTCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACACCCTGATCCTCTCCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACGGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTCCGCGGATATCGGCCAGACTCTGGCAAAatat                                                               <  1:110290/250‑1 (MQ=255)                                                                                                                                                ccGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATAtt                                                              <  1:181242/251‑1 (MQ=255)                                                                                                                                                    cgcgACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTg                                                           <  1:22489/250‑1 (MQ=255)                                                                                                                                                        tgttagtcggtactGCCGCGGGGCTGGTACTCGTCCACCTCCTTCTGCCGGAGCTCATGGCGCTTCTACGCGAGTACGACATCCGGATCCTCTCCGCTGACCTCGGTTGCGAGCCTTGCTGGTCCGGTCCTGACCCCACGCGTTACTACAGTCCGCTACTTGTATATGGCCGGAAAGTAAAACCGGGCTCATTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGTCAAAATTTTTTGGTAc                                                       <  2:267770/237‑1 (MQ=255)                                                                                                                                                            cGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTg                                                  >  1:122014/1‑251 (MQ=255)                                                                                                                                                                      gCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTT‑GGTACTTCTGatatttaata                                        <  1:79475/250‑7 (MQ=255)                                                                                                                                                                          cgGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGAACCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACCCGCGGGAACCCATTCCGATACTGGTATATGGCCCGAAAGTAAAACCGGACTCACTGGGTCATCGTGAAACATTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAAAATGGc                                    >  2:10299/1‑251 (MQ=255)                                                                                                                                                                                  gAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCAt                            >  1:167414/1‑251 (MQ=255)                                                                                                                                                                                       gTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGTTATGGAATATGGCAAAGCCATGTTCt                       >  1:265782/1‑251 (MQ=255)                                                                                                                                                                                                            gCTGATGTCTCTGCTACGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCTAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGtt  <  1:2556/251‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            ACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACAC‑CATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTT  >  NC_000913/4620393‑4620845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑