Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,238	T→G	intergenic (+445/+452)	insO → / ← fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,238	0	T	G	100.0%	52.5 / NA	21	intergenic (+445/+452)	insO/fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/12);  total (9/12)

TTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCC  >  NC_000913/4510002‑4510481                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    ttCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGAtt                                                                                                                                                                                                                                       >  1:164088/1‑251 (MQ=255)                       aaGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGa                                                                                                                                                                                                                 <  1:254745/251‑1 (MQ=255)                              ggCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATcc                                                                                                                                                                                                          >  1:60371/1‑251 (MQ=255)                                           tCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCATGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGTATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCAAAc                                                                                                                                                                                             >  2:226961/1‑251 (MQ=255)                                                 tATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTACAGGGTTGCTTTTGATTTACGTTGGCGTATGATCATTGATTTATCCTCAAAAGCCCAACCTCAtt                                                                                                                                                                                       >  1:101669/1‑251 (MQ=255)                                                    gCCAGCGTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGt                                                                                                                                                                                    <  1:185024/251‑1 (MQ=255)                                                             ggTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCa                                                                                                                                                                           <  2:164088/251‑1 (MQ=255)                                                                 aGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTCCCGGCGATTAAGAAAGCTGCACCTACCTAAGTCGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGAGTCTGATCATTGATTTATCCTCAAAAGCCCAAACTCATTGGTAATGACCCCGCTc                                                                                                                                                                       >  1:179163/1‑251 (MQ=255)                                                                  gCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGGGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTcc                                                                                                                                                                      <  1:275601/251‑1 (MQ=255)                                                                                                cATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGCAACTGGCTTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCAt                                                                                                                                                                                                                    >  2:214359/1‑175 (MQ=255)                                                                                                cATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCAt                                                                                                                                                                                                                    <  1:214359/175‑1 (MQ=255)                                                                                                       aaCAATAGATCCATCAACGGACCTGTATAACGTGGCTGCCGGAGATTACGAAAGCTACACATACCTAAGTAGTAGCCAACGAACACTTTTTAGCAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTAGCTTTAAGTTGGAGTCTGATCAGTGATATATCATCAAACGTACAACCTACTTGGTAATGAACCAGCTCCGATAATGTCCGCCATGACACAGAGAACAATTTTTTgt                                                                                                                                 >  2:278143/1‑250 (MQ=255)                                                                                                              gATCCATCACCTGGCCTGTATAACGTGTCTGCCGGTGATTAAGAAAGCTGCGCCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGAGCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGATCCAGATCCGTGAATGTCCGCTCCGGCACAGAGCGAAATTTTTTGATCTcccc                                                                                                                          <  2:207870/251‑1 (MQ=255)                                                                                                                                   aacggtgCTGCCGGTGAATAAGAAAGCTGCACCTACCTAAGTAGGAGCAAACGCACACTTTTGAGAAAAAGCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGTTTGCTTTTGATTTACTGTGGCGTCTGATCATTGATTGATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTACGCTCTGGCACAGATCGAAATTTTTTGATCTCCCCTCTGAAATCTAAACTTAGTc                                                                                                      <  2:294032/244‑1 (MQ=255)                                                                                                                                     cGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATg                                                                                                   >  1:42047/1‑251 (MQ=255)                                                                                                                                                                       ctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                                                                                                                                                                                                     >  1:261427/1‑103 (MQ=255)                                                                                                                                                                       ctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                                                                                                                                                                                                     <  2:261427/103‑1 (MQ=255)                                                                                                                                                                                         acacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAg                                               <  1:7478/251‑1 (MQ=255)                                                                                                                                                                                                           gATGGTCATAAACTTGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCTCAATCTCAGTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGATATTTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAATTTAACTGCGCGTTTTTCGTTGCCGTTGGGGTTATACAGACATGTcc                             <  2:259905/251‑1 (MQ=255)                                                                                                                                                                                                              ggTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCGCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTTAATGTCCGCTCTGGTACAGAGCGAAATTTTTTGATCTCCCCGCGGAAATTTAAACTTGGTCATGTCACGTTTTTGGGTTTCTAAAATGTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGtt                          <  1:44134/251‑1 (MQ=255)                                                                                                                                                                                                                                      cGTTCCAGGGTTGCTTGTGATTTACGTTGGCTTCCGATCATTGATTTAGACTCATAAGCCCAACCGCATTGGTAATGAACCATCTCCGTGAATGTCCGCTCTCGCACAGAGCGGAATTTTTTGATCTCTCCCCTGAAATCTAAACGTAGTCATGTCACGTGTTAGGGATTCGAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGcc  <  2:24303/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    TTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCC  >  NC_000913/4510002‑4510481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑