Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,493	T→C	F19F (TTT→TTC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,493	0	T	C	92.3%	36.9 / NA	13	F19F (TTT→TTC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (4/8);  minor base A (1/0);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGT  >  NC_000913/4475261‑4475670                                                                                                                                                                                                                                         |                                                                                                                                                                                  cagacgttgctcttccgatctCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGt                                                                                                                                                                  <  1:132498/230‑1 (MQ=255)      ggtgctaGCCCAGGTTCGCAAAGAAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGACGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGTCGTACTTATTCACTCAAGGAGTGCGTCATGAGCAAAATATCATATTTAAATTATTCTCAACACATTTCATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTaaa                                                                                                                                                            <  2:293602/245‑1 (MQ=255)       gcgcCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTGCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCATATTTAAATTATTCGCAACACATTACATTAGCCGACAATGTCAAACAAAAAAGTGAATTTTTAAAt                                                                                                                                                           <  1:187110/251‑1 (MQ=255)                      cGCAAAGCAGAAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCCGCTTTAAATTATTCTCAACACATTACATTAGCCGACAATTTACAACAAAAAAGTGAAGt                                                                                                                                                                  >  2:132498/1‑229 (MQ=255)                                                ggCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATGCCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCg                                                                                                                   <  1:259991/250‑1 (MQ=255)                                                                   gTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTTCCGGGGGGCAGGATAACAGAAgg                                                                                               >  1:260242/1‑251 (MQ=255)                                                                    tAAATAACTCAATGTTTAAGGCGAGTTTGTGAAAAGATCGTGTTTTGATATTGTTTTGTGAGGAAAAATTGTAATGCGTAGACGTTTGTAGTCACGCAAGGAGGTCGTCAAGAGCAAAATATCAGATTTAAATTATTATCAACTCATTACATTAGCCGACAATTTCAAACAAAAAAGTTAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGGCGGGGGGCAGGATAACAGAAgg                                                                                               <  2:258091/250‑1 (MQ=255)                                                                                      cgacGAGGTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAAAGCTTAGACGTTTTTATTCACTCAAGGAGTTCGTCATGATCAAAATATCAGATTTAAATTATTATCAAGAAATTACATTCGCCGACAATTTCAAACAAAAAAGTGACGTTTTAAATACATGGCGATTTGGAATGAATGATTTTGCCCGTATTACCGGGGCGAAGTGTAAAAGAAGGAAAAttctttgtcatgt                                                                              >  2:243623/4‑242 (MQ=255)                                                                                                                                    aaaTTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGACGTTTTAAATAACTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGACGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTgg                               >  1:32184/1‑250 (MQ=255)                                                                                                                                               gCTTTGACGTTCTTATTCACTCAAGGAGTACGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATGTACCCTGGGTTATGTGGATTTTAGCAAAc                   <  2:253122/251‑1 (MQ=255)                                                                                                                                                cTTAGACGTTCTTATTCACCCAAGGAGTTCGTCCTCAGCACAATATCAGATTTAAATTATTCTCAACACATTCCATTAGCCGACAATTTCAAACAAAAAAGTGACGTTTAAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTTCCGGGTGGCAGGATAACAGTAGGAATATTCTTTCTCCTGGCGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGTTTTTGTGGATTTTAGCaatgg                  >  2:208572/1‑248 (MQ=255)                                                                                                                                                            tagtCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACAGTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAAGACCTGGCGTGGTGGAATTAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGaa        <  2:291965/246‑1 (MQ=255)                                                                                                                                                                 aatCAAGGATTTCTGCATGAGCAAAATATCAGATTTAAAATATTCCCAACACATTACATTAGCCGACCAATTCAAACAAAAAAGTGAAGTGTTAAATTCCTTGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGTCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTGTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGACGCGGGt  <  2:120286/248‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                  GTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGT  >  NC_000913/4475261‑4475670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑