| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,473,900 | C→T | G52R (GGA→AGA) | bdcA ← | c‑di‑GMP‑binding biofilm dispersal mediator protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,473,900 | 0 | C | T | 93.1% | 80.4 / ‑5.7 | 29 | G52R (GGA→AGA) | bdcA | c‑di‑GMP‑binding biofilm dispersal mediator protein |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (16/11); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATG > NC_000913/4473662‑4474126 | ccGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAACGACAGCGTCTCTGTCCGCACTATCTGTGAATACTGCTGTCGCTCCAGtctcgtgagc > 2:255643/1‑245 (MQ=255) gATGCGCCCGCCTGCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTTAAAAGGCGATCAATATCGGCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAg < 2:291104/251‑1 (MQ=255) gcgcCCGCCTTCGTGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGCCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGc < 1:170328/249‑1 (MQ=255) ccggcttCTGCTGGGCGGCTTAAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGAGATTAATATCATCGTGATCTCATTCCATGGGTGCGCCAAAGAAGCCAATCCCTGCATTTACCACCAGGATATCCAAGGCTCCGCTCTTACGAACGTCAGCAATGTAAGAGTCTCTGTCAGCATGATCTGTGAATCTTGCTGTCGCTATAGTCTCTGGAGCCAG‑GCGTTTTGCGGCATCTTTCGa < 2:250245/244‑1 (MQ=255) tCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGcc > 2:226845/1‑250 (MQ=255) agGCACGATAAGGAGCGTGCATATTGATTTTGAAAAGACGATCAATATCGTCGGCATTTAATTACAGGGCCTGGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGACCGACATCAGTGACAGCGTCTCTGTCTGCAATATCTGGGAATACTGCTGTCGCTCCAGTCTCTTGTGC‑AGAGCGATTAGCGGCATCTTTCGACCCCGctacgatgaatgacaatttggcc > 2:17701/1‑229 (MQ=255) gCATGATAAGGAGCATGAATATTGATTTTGAAAAAGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCACCAAAGACGCCAATACCTGAATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGCAATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGcccc > 1:153589/1‑251 (MQ=255) gATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTATGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCg < 1:177919/251‑1 (MQ=255) aaatTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacgacg < 2:102211/249‑1 (MQ=255) aatGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCACGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGGTCTTCCGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTGGTGCCAG‑GTGTTTAGCGGCTTCTTTCGACCCCTCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTa < 1:283259/249‑1 (MQ=255) cGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGAt > 1:97782/1‑251 (MQ=255) tcgGCATTTAATTCCATGGCCTCGCCAAAGACGCCAATCCCTGCAATGACCCTCAGGATATCCAAAGCGCCGCTCTTACGAACGTCATCAATGCCCGTGTCTTTGGCATCACTAACTGTGAATACTGCTGTCTCTCTAGTCTCTTGTGCCAG‑GCGTTGAGCTGCTTCTTGCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACgtct < 2:265341/248‑4 (MQ=255) ttCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATg > 1:189477/1‑250 (MQ=255) gCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGt < 1:226845/251‑1 (MQ=255) aaGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGt > 2:292737/1‑251 (MQ=255) gACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAAAGACATCAATGACAGCGTCTCTGTCAGCACTAACTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑TCGTTTAGAGGCATATTTCGACCCCGCATAGGTGAATCGTACATTTGCCCCATCGTTGACGACACTACGTACGATACCGTCACCGATACCAGCACTGCCACCGAGGATGCGAACTATTGTACCTGTa > 2:76068/1‑250 (MQ=255) cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAgcg > 1:200254/1‑251 (MQ=255) tACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGccc > 1:293607/1‑251 (MQ=255) ggATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAACCGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAttt > 1:282365/1‑251 (MQ=255) atatccaaatctccgctctttctgacgaCATCAATGTCATCGTCTCTGTCAGCACTATCTGTGAATACTTCTGACGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGTCATTTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGTCGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGTGGATGAGAACTTTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttat < 2:294181/227‑1 (MQ=255) cAATGCTCCGCTCTTTCGAACGACATCAATGACAGAGTCTCTGTCAGCTCGATCTGGGAATTCTGCTTTCGCTCTAGTCTCTTGTGCCTG‑GTGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCCGCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGTCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGAATCCTTCATTTATTATGt < 2:257845/251‑1 (MQ=255) cgccgcTCTTACGAAAGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGACCTGTCTTACCTGTAAAAGAGCCCATAAGGACCCCTTGATTAATTATGTAACAt > 2:293321/1‑251 (MQ=255) cgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGCTTTATTATGTAACATGc > 1:247851/1‑250 (MQ=255) cgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTCGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCa < 1:159174/251‑1 (MQ=255) gaacgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAAc > 1:96660/1‑251 (MQ=255) cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGCGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAAc > 2:6024/1‑251 (MQ=255) aGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGt > 1:265823/1‑251 (MQ=255) cAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGtttt > 2:177463/1‑251 (MQ=255) tATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATg > 1:284944/1‑251 (MQ=255) | CCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAG‑GCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATG > NC_000913/4473662‑4474126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |