Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,437,8900CT84.2% 34.1 / ‑0.8 20Q62* (CAG→TAG) ytfIprotein YtfI
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (11/5);  total (12/8)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACG  >  NC_000913/4437656‑4438113
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                               
gTGAGATATTTAATAGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACCATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTACCTTTAAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTgg                                                                                                                                                                                                                 >  2:116538/1‑251 (MQ=255)
               gCGACATAAAGTAACCAAATAAAATAATGTGGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATTCATTTAAGAAAAAATATTGTATTTCTAATTATGATAGTCTGTTAACTTTGATGGAGTTCTTTCGTTGCGCAACTTCGATTGATAAAAGTGGTTATCAGGTGCttgc                                                                                                                                                                                                  <  2:224037/251‑3 (MQ=255)
                              cAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTa                                                                                                                                                                                                              <  2:292507/224‑1 (MQ=255)
                              cAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTa                                                                                                                                                                                                              >  1:292507/1‑224 (MQ=255)
                              cAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTTCTTTCATCAAAAAATGAAAc                                                                                                                                                                                   >  2:85840/1‑251 (MQ=255)
                                        aaggttgtaaaaaggtacaagggATCCGACAAAATAATTTTATTGGTGCGTTGGAGTTAATTGTAAAGCCTACGTATACAAAAACACATTCAAACAATTTTTTTAACCACATCCCACATGCATTACAGAAAAAAGATTGAATTTCGAATTATGATACACTGTTAACTTTGAGGGAATTCTTTCGTTACTCAACTTAGATAGATAAAAGTAGTTATCAGGTGCTTTCTTCAAAACATGAATGTGTTCAcccc                                                                                                                                                                         >  2:254790/22‑248 (MQ=255)
                                               tCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCATGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGAt                                                                                                                                                                   >  1:256176/1‑250 (MQ=255)
                                                                                            ggAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTGTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAatat                                                                                                                      <  1:30308/250‑1 (MQ=255)
                                                                                                 tATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTCTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATg                                                                                                                >  1:137298/1‑251 (MQ=255)
                                                                                                        aaaGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGACAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGc                                                                                                         >  2:128054/1‑251 (MQ=255)
                                                                                                                        aaaaCACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAAAAAAAAGATTGGATTTAGAATTCTGATAGCCTGTTAACTATGAAGGAGTTCTTTCGTTGCGCAACTTAGATTGATAACAGTGGTAATCAGGTTCTTTCATCAAAAAATGAAACTGTTCAAGCCATGGATAAGTTTATTATCAGTTTCAGCCATAAAGCTAACGGTACCGAATATACAATGACATTGCGAGGTAGTGGATTTg                                                                                          >  2:50338/1‑250 (MQ=255)
                                                                                                                         aaaCACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGAGAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACGCAGATTGATAAAAGTGGTTATCAGGTGCTGTCATCAAAAAATGAAACTGGTCACGCCATGGATAAGTTTCTGATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATTACATTGCGAGGTAGTGGACTTGaa                                                                                        <  2:137389/251‑1 (MQ=255)
                                                                                                                          aaCACCTTAAAACAATTTTTTTAACAACCTCCATCATGCCTTTACGACAAAAGATTGGATTTCGAATCATGCTAGCCTGTTAACTTTGAGGGAGTTCTTTCGTAGCGCCACTTAGATTGATAAAAGTGATTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCAATGGATATGTTTCTTATCAGTTTCAGCCTTACAGATAACGGTGCCGAATATACCAGTACATTGCAGGGTAGTGGATTTGAAt                                                                                       >  2:149134/1‑251 (MQ=255)
                                                                                                                          aaCACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAAt                                                                                       <  1:154345/251‑1 (MQ=255)
                                                                                                                                           tttttAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGTATCAAAGATTGATCAACGTGGTTAACAGGTGCTTTCATCAAAAAAAGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAACCTTAAAGATATCGGGGTCGAATAGTCAATGATATTGCGAGGTAGTGGACTTGAATTCGAAGAAATACTCAt                                                                       <  2:165915/250‑1 (MQ=255)
                                                                                                                                               tAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACa                                                                   >  1:164936/1‑250 (MQ=255)
                                                                                                                                                        ccatcATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTGGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATGTGAATACGAAGAAATACCCATAACAATAAATGaa                                                          <  2:76146/250‑1 (MQ=255)
                                                                                                                                                            caagCATTTAAGAAAAAAGATTGGATTTCGAGTTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGGGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAAtata                                                      >  2:198932/4‑250 (MQ=255)
                                                                                                                                                                         aaaaaGATTGGATTTCGAATGATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTGAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGAt                                        <  1:116538/251‑1 (MQ=255)
                                                                                                                                                                                                                tGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGACTTTCCGTTAGAACAAAACAGACg  >  2:264161/1‑250 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                               
GTGAGATATTTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACG  >  NC_000913/4437656‑4438113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑