Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,217,310	A→G	S68G (AGC→GGC)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,217,310	0	A	G	93.5%	76.0 / ‑1.6	31	S68G (AGC→GGC)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (15/14);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

GCCTGTTAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCG  >  NC_000913/4217066‑4217553                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gCCTGTAAGGGTAAACCGCCACGTAACTATGGAGCATCTGCACATGAAAACCCATACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAAGCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTTAGGCGAAAAAAGGCTACATCAACGGCCTCg                                                                                                                                                                                                                                                 <  2:49971/251‑1 (MQ=255)             aaaCCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCactgact                                                                                                                                                                                                                                      <  1:273822/250‑1 (MQ=255)             aaaCCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGATGGGAAGGCATTACTCGCCCATACAGTGAGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCCGCGAAACTGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCactgactg                                                                                                                                                                                                                                     >  1:222393/1‑251 (MQ=255)                   ccacATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTc                                                                                                                                                                                                                               >  1:240894/1‑251 (MQ=255)                    cacacaACTATGTAGCATCTGCACATGAAAACCCTTACACAACAAATTGAAGAATTACAGAAAGAGTGTACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCTCAACTGGGCGCAGCTAAAATGTGGCGTCTGCTGCCCGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGTCGCACTGACTGGCGGTC‑a                                                                                                                                                                                                                             <  2:191754/251‑1 (MQ=255)                          aCTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAAATGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAACAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑Agacg                                                                                                                                                                                                                         >  1:293583/1‑246 (MQ=255)                               ggAGCATCTGCACATGAAAACCCGTACACAACAACTTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCCCTGACTGGCGGTC‑AAgcgcctgaac                                                                                                                                                                                                                  >  2:150287/1‑245 (MQ=255)                               ggAGCACCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAGCTGGGCGCAGCGAAAATGTGGCGTCGGCTGCTCGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACCGACTGGCGGTC‑AGTCTCTGCAAc                                                                                                                                                                                                                  <  2:270275/251‑1 (MQ=255)                                  gCATCTGTCCATGAAAACCCGTGCTCTACAAATTGAAGAATTACGGAAAGAGGGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGAGGTGGTGAAGTTACGCGGTTCAGTAAATCCTGAATGCTCGGTGGCGCAACTGGGCGCAGCGAAAGTGTGGCGTCTGCTGCACGGGGAGTCGAAGAAAGTCGACATCAACGGCCTCGTCGCACTGAGTGGCGGTC‑AGTCGCTGCAACAgg                                                                                                                                                                                                               <  2:181989/251‑1 (MQ=255)                                          acaTGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCCCTGACTGGCGGTC‑AGGGGCTGCACCAgggtaaag                                                                                                                                                                                                         >  2:1019/1‑243 (MQ=255)                                               aaaaCCCGTACACAACAAATTGAAGAAGTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGGGGTTAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTGCATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTAt                                                                                                                                                                                                  <  1:15127/251‑1 (MQ=255)                                                      gTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCa                                                                                                                                                                                           <  1:149340/251‑1 (MQ=255)                                                               aaaTTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGGCTATCtt                                                                                                                                                                                  >  1:274254/1‑250 (MQ=255)                                                                                              aCCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTc                                                                                                                                                                                <  1:18276/222‑1 (MQ=255)                                                                                                   gTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGAACGGTGAGTCGAAAAAAGGCTACATCAACGGCATCGGCGCACTGACTGGAGGTA‑CGGGGGTGGAGAAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGACGGGAGGTAGcggcgggagctaaacgggcg                                                                                                                                              >  1:136944/1‑237 (MQ=255)                                                                                                         aaGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCCCGCTGGCGCAACTGGGCGCAGCGACAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCCAGG‑GCCGCAACAAGCCAAGACGGGCTTTGGAGAAGTCTAACTGTTGGGACGGCAGGCAGAGGCGGagcccaacacgtgcgccc                                                                                                                                          >  2:85435/1‑232 (MQ=255)                                                                                                         aaGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGc                                                                                                                                       >  1:43477/1‑251 (MQ=255)                                                                                                            gCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGCCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATg                                                                                                                                    <  1:41980/251‑1 (MQ=255)                                                                                                                            cAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAAAAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGAAGCT‑AACCTGGCGGCCAGCATGTATCCGGGTCAGTCGc                                                                                                                    >  1:21783/1‑251 (MQ=255)                                                                                                                                                    aCGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGCAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAGGGCAACATCAACGGCCTCGGCGCACTGAATGGCGGCC‑AGGCGGCGCAACAGGCGGAAGCGGGAAATGAAGACGTCTATCTGTCGGGGCGGCAGGTAACCGCGGACGCT‑AACCCGACGGCCCGCAAGTATCAGGATAAGCGGCTCTACCCGCCAAACTCGGTGcacc                                                                                            >  1:22874/1‑248 (MQ=255)                                                                                                                                                    aCGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGGATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGcccg                                                                                            >  1:173906/1‑249 (MQ=255)                                                                                                                                                      gcgGGTCAGTCAAACCGGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCTAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTAATGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑ATCCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGTTGCCAGCt                                                                                          <  2:156676/251‑1 (MQ=255)                                                                                                                                                      ccggtgtaGTCAATCCTGACGGCTAGCTGGCGCAATTGGGCGCAGCGAAAATGGGGCGTCTGCGGCACGGAGAGTTGAAGAAAGTCTACATCAAAGGCCTCGGCGCACGGTCTGGCGGTC‑AGGCTTTGCAACAGGCGAAAGCGGGTATTGAAGCATTCTAGCGGTCGGTATTTCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATTAGTCTTTCTATCCGGCAAACTCGGAGCCAGCt                                                                                          <  2:293583/244‑1 (MQ=255)                                                                                                                                                            cAGTTAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGTCGGCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCTACGGCCCCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGGCGGGATGGCAGGTAGCGGTGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAATCTCGGTGCCAGCTgtggtg                                                                                    <  1:285190/251‑1 (MQ=255)                                                                                                                                                                        aaTGCACGCTGGCGCAACTTGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCTCCGGCGCCCTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGTTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGGATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATcaac                                                                        <  1:256496/251‑1 (MQ=255)                                                                                                                                                                                           gggCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAACAGTCTATCTGTCGGGATGGCAGGGAGCGGCGGCAGCTTAACC‑GGCGGGCCGCATGTATCCGGGTCAGTCCCGCTATCCGGCACACCCGGGGCCCGCCGTGGGGGGGCGGGTCAAACACACCCTCcgtccggacc                                                     >  2:213649/1‑245 (MQ=255)                                                                                                                                                                                                         tgtgGCGTCTGCTGCACGGTGCGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGGATTGAAGCAATCTATCTGTCGGGATGGCAGGAAGCGGCGGACGCT‑AACCTGGCGGCACCCAACTATCCGGGTCAGGAGCGCCATCCGGCAAACACGGTGCCAGCTGCTGTGGAGCGGAGAAAAAACACATTCCGGCGGGCCGCTCAGATACAATgg                                       >  1:169911/1‑251 (MQ=255)                                                                                                                                                                                                             gCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCcg                                   <  1:8063/251‑1 (MQ=255)                                                                                                                                                                                                                          ggTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGcc                      <  1:150287/251‑1 (MQ=255)                                                                                                                                                                                                                                            tACATCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGACGCAACAGGCGGAAGCGGGTATTGGAGCCGTCTATCTGTCGGGATGGCAGGTAGAGGCGGACGCT‑AACCTGGCGGGCAGAATGTATCCGGGTAAGACGCTCTATCCGGCAAACTCAGGGCCAGCTTTGGGGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCACAGGGCCGCGGGCATTGAACCGGGCGATCCGCGCTCTGt    >  1:259936/1‑251 (MQ=255)                                                                                                                                                                                                                                               aTCAACGGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTTTCCGGCAAACTCGGTGCCCGCTGTGGGGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGAACCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCg  >  1:110237/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GCCTGTTAGCGTAAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTC‑AGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCT‑AACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCG  >  NC_000913/4217066‑4217553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑