Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913968,8370GA87.0% 59.2 / ‑2.1 24D158N (GAT→AAT) lpxKtetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (7/13);  total (8/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

GGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGT‑ACGCGTTGTGACGTTGCTCAGCTTG‑AACATGTAGTGGCGATGGCGGGGATTGG  >  NC_000913/968591‑969081
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
ggTAGTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGACGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAAtgt                                                                                                                                                                                                                                                    <  1:293022/251‑1 (MQ=255)
   aatcgtgTGGGGGTCGTATCGCTGGGATATGGTTGTAAGGCTGAATCCTATCCGCTGTTATTGTCGGCAGATACCACAACAGTACATGCGGGTGATGAACCTGTGTTTATTTATCAACGCACTGATGCGCCTGTTGCGGTTTTGCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTgg                                                                                                                                                                                                                                                  <  1:134103/244‑1 (MQ=255)
           gggggTCGTATCGCGGGGATATGGTGGTCAGGCTGAATCTTAACCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATATATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTGTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCg                                                                                                                                                                                                                                         <  1:59020/251‑1 (MQ=255)
                             atatGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTTGTTTATCAACCCACTTATGCGCCTGGTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCCACACCCTGATGTGCAGATCATCGTAACCCACGACGGTTTACAGCATTACCGTCTGGCGCGTGGTGTGGGAATTGTCGTTTTTGGTGGGGTTCGTc                                                                                                                                                                                                                       >  1:92981/1‑251 (MQ=255)
                                 ggtggtAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCCCTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGGGGAAATTGTCGTTATTGATGGTGTGCGTCGCtt                                                                                                                                                                                                                   <  1:88532/251‑1 (MQ=255)
                                    ggtAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCTCGACACCCTGATGTGCAGATCATCGTAGCCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTg                                                                                                                                                                                                                 <  1:155067/250‑1 (MQ=255)
                                          gCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATgg                                                                                                                                                                                                          <  1:228157/251‑1 (MQ=255)
                                                   tATCCGCTGTTATTTTCGGCAGATACCACAAAAGCACAGGCGGGTGATGAACCTGTGTTGAGTTATCAACGCACTGATGCGCCTGGTGCGGTTTCGCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACCCCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGGGCGTCGCTTTGGCAATGGCtggtgg                                                                                                                                                                                                   <  2:13049/249‑1 (MQ=255)
                                                   tATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCtggt                                                                                                                                                                                                     <  1:276703/247‑1 (MQ=255)
                                                    atcctcagttattggtggcgtaTACCTCAACGGCGCATGCGGTTGATGTACCTGGGTTGATTTATCAACGCACGGTTGCTCCGTTTGTGGTTGCGCTCGTTCGTTCTGATGCGGTAAAAGCCAGTCTTGCGCAACTCACTGATGTGCAGATTATCGTAACCGACTACTGTTGACAGCATGACCTTCTGGCTCGTAATGTGGAAATTGTCGTTAGTGATGGTGTGCGTCGCTTTGGCAATGGCTGGGGGTTg                                                                                                                                                                                                <  2:271564/230‑1 (MQ=255)
                                                                                          gCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGGGCGGGGGGCttta                                                                                                                                                           >  1:294637/1‑248 (MQ=255)
                                                                                                 atgaACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGtt                                                                                                                                                   >  1:204638/1‑251 (MQ=255)
                                                                                                  tgaACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGGCAATGCGGGAGCGAGAGGGGCGCTTATAGTCgggtg                                                                                                                                                  >  1:271407/1‑248 (MQ=255)
                                                                                                             tGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAAAGCGTGGGCGAGCGGGGGGCTTTAAGTCGGTTTGTGCGGTAATc                                                                                                                                       >  1:179599/1‑251 (MQ=255)
                                                                                                                             tGATGCTCCTGTTGCGGTTACTCCCGTTCGTTCTGAGGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAg                                                                                                                                                         <  2:253618/217‑1 (MQ=255)
                                                                                                                             tGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGGGCGAGCGGGGCGCTTAAAg                                                                                                                                                         >  1:253618/1‑217 (MQ=255)
                                                                                                                                                   cccGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATccc                                                                                                    <  1:236808/248‑1 (MQ=255)
                                                                                                                                                          gttcTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTc                                                                                                                                                       <  1:143412/190‑1 (MQ=255)
                                                                                                                                                                     gTAAAAGCCATTCTGGCGCAACACCCAGATGTGCAGATCATCGTAACCGACGACGGTTGACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCa                                                                               <  2:32652/251‑1 (MQ=255)
                                                                                                                                                                       aaaaGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTGTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGTGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGc                                                                                                               <  1:121505/217‑1 (MQ=255)
                                                                                                                                                                                                         atcatcGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGT‑ACGCGTTGTGACGt                                          <  1:264237/251‑1 (MQ=255)
                                                                                                                                                                                                                 aaCCGACGACGGTTTACAGCATTACCGTCTGGCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGGGCTTAAAGTCGGTTGATGCGGGAATCGTCAACGGCGGTGTCCCTCGCAGCGGGGGAATCCCCCAGGATCTCCTGCCGGGGCAGGGGGGGGATTTACGTACCGGTTACG‑GTTGTGACGtttgtccgc                                  >  1:135074/1‑244 (MQ=255)
                                                                                                                                                                                                                                              gggCGCGTCATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGGGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGT‑ACGCGTTGTGACGTTGCTCAGCTTG‑AACATGTAGTGGCGATGGCGGGGAtt    <  2:294637/250‑1 (MQ=255)
                                                                                                                                                                                                                                                gcgcGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGGGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGGGGCCCTCGCAGCGGGGGAATCCCCCAGCATCTGCTGCCGGGTCCGGGGGGGGAATTACGTGCCCGG‑CCGCGTTGTGGCGTTGCTCAGCTTGTAAC‑TGTAGTgggggtgggggggggttg  >  1:38411/1‑235 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
GGTATTCGCGTGGGGGTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACCGGT‑ACGCGTTGTGACGTTGCTCAGCTTG‑AACATGTAGTGGCGATGGCGGGGATTGG  >  NC_000913/968591‑969081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑