Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,133,311 G→A P232P (CCG→CCA metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,133,3110GA95.0% 54.5 / ‑3.8 20P232P (CCG→CCAmetF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (9/10);  total (9/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATCACACAGT  >  NC_000913/4133070‑4133536
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 
aaccGGAAGCTCACCCGGAAGCAAAAAGCGCTCCGGCAGATTTGATTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGAt                                                                                                                                                                                                                           <  2:251679/248‑1 (MQ=255)
          cccccctgAAGCAAAAAGCGCTCAGGAGGATTTGCGTAATCTGAAACGCAAAGTTGATGCCGGATCCAACCGCGCGATTACTCATTTCTTCGGCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTTGAAATTATTCCGGGAAGTTTGCCGGTATCTAACTTTAAACAGGCGAATAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGt                                                                                                                                                                                                                <  2:258601/244‑1 (MQ=255)
                      aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTGATGTGGGAATTATTCCGGGGATTTTTTCGGGATCTAACTTTTAAAAGGCGGAGAAATTTTTCGATATGACCAACGAGCGTATTCCAGCGGGGATGGGGCAAATGTTCGACGGTCTgg                                                                                                                                                                                                    >  1:104631/1‑251 (MQ=255)
                                      gATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCACTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAAc                                                                                                                                                                                    <  1:132918/251‑1 (MQ=255)
                                                              gTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGGTGTGGGAATTATTTCGGGGATTTTTCCGGTATCTAACTTTTAAAAAGGGAAGAAAATTTCCGATATGACCAAAGGGCGTATTTCAGCGGGGGGGGGGCAAAAGTTTGGCGGTGTGGGTGATGAAGCCGAAAACCCCAAAATGGGTgggggggatat                                                                                                                                                            >  1:143302/1‑242 (MQ=255)
                                                                ggaggCCGGCGCCAACCGCGCGATTACTCAGTTCTTCGTCGATGTCGAAAGCGACCGGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGGGGAAATTACTCCGGGAATTTTGCCGGTATCTAACGTTAAACAGGCGACAAAATGTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGATACCCGCAACCTGGTTGGCGCGAATAttc                                                                                                                                                          <  2:8495/247‑2 (MQ=255)
                                                                     ccGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGTTGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTAGCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATg                                                                                                                                                     <  2:183352/251‑1 (MQ=255)
                                                                                                       cGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCTGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTg                                                                                                                    >  1:188462/1‑250 (MQ=255)
                                                                                                                     aCCTGTGTTTTCGGGACCGCTGTGTTTCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACtt                                                                                                      <  2:250282/250‑1 (MQ=255)
                                                                                                                            ttttCGTGACCGCTGTGTACCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTAGCCGGTATCTATCTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACg                                                                                               <  2:58172/250‑1 (MQ=255)
                                                                                                                                                        aTTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCtt                                                                                            <  1:225754/225‑1 (MQ=255)
                                                                                                                                                        aTTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGAGAAGGAGTGAAAGATTTCCACTTCTATACGCtt                                                                                            >  2:225754/1‑225 (MQ=255)
                                                                                                                                                          tGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGAtt                                                                 <  2:105122/250‑1 (MQ=255)
                                                                                                                                                                      tattCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCAGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGCCGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTACACTTCTATACGCTTAACCGTGCTGAAATGAGATACGAGATTTGCCATACGCTgg                                                    >  2:284559/1‑251 (MQ=255)
                                                                                                                                                                                       cgttATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCATCGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGTAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGTTTAGACCTGGTTta                                   <  2:271379/248‑1 (MQ=255)
                                                                                                                                                                                                   ttAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTTGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAgtgt                         <  2:143302/249‑1 (MQ=255)
                                                                                                                                                                                                              gaagaaATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAACTGAGTTACGCGATTTGCCATACGCTGGGGGTTCGCCCTGGTTTATAATTAGTGTGGCTTTTGTGa              >  1:2768/1‑250 (MQ=255)
                                                                                                                                                                                                                 gaaATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAAt          >  1:222986/1‑251 (MQ=255)
                                                                                                                                                                                                                   aaTTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATc         >  1:64012/1‑250 (MQ=255)
                                                                                                                                                                                                                         ccGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATCACACAgg  >  1:254313/1‑250 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 
ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATCACACAGT  >  NC_000913/4133070‑4133536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: