| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,133,311 | G→A | P232P (CCG→CCA) | metF → | 5,10‑methylenetetrahydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,133,311 | 0 | G | A | 95.0% | 54.5 / ‑3.8 | 20 | P232P (CCG→CCA) | metF | 5,10‑methylenetetrahydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (9/10); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 | |||||||||||
ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATCACACAGT > NC_000913/4133070‑4133536 | aaccGGAAGCTCACCCGGAAGCAAAAAGCGCTCCGGCAGATTTGATTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGAt < 2:251679/248‑1 (MQ=255) cccccctgAAGCAAAAAGCGCTCAGGAGGATTTGCGTAATCTGAAACGCAAAGTTGATGCCGGATCCAACCGCGCGATTACTCATTTCTTCGGCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTTGAAATTATTCCGGGAAGTTTGCCGGTATCTAACTTT‑AAACAGGCGAATAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGt < 2:258601/244‑1 (MQ=255) aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTGATGTGGGAATTATTCCGGGGATTTTTTCGGGATCTAACTTT‑TAAAAGGCGGAGAAATTTTTCGATATGACCAACGAGCGTATTCCAGCGGGGATGGGGCAAATGTTCGACGGTCTgg > 1:104631/1‑251 (MQ=255) gATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCACTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAAc < 1:132918/251‑1 (MQ=255) gTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGGTGTGGGAATTATTTCGGGGATTTTTCCGGTATCTAACTTTTAAAAAGG‑GAAGAAAATTTCCGATATGACCAAAGGGCGTATTTCAGCGGGGGGGGGGCAAAAGTTTGGCGGTGTGGGTGATGAAGCCGAAAACCCCAAAATGGGTgggggggatat > 1:143302/1‑242 (MQ=255) ggaggCCGGCGCCAACCGCGCGATTACTCAGTTCTTCGTCGATGTCGAAAGCGACCGGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGGGGAAATTACTCCGGGAATTTTGCCGGTATCTAACGTT‑AAACAGGCGACAAAATGTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGATACCCGCAACCTGGTTGGCGCGAATAttc < 2:8495/247‑2 (MQ=255) ccGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGTTGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTAGCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATg < 2:183352/251‑1 (MQ=255) cGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCTGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTg > 1:188462/1‑250 (MQ=255) aCCTGTGTTTTCGGGACCGCTGTGTTTCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTTGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACtt < 2:250282/250‑1 (MQ=255) ttttCGTGACCGCTGTGTACCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTAGCCGGTATCTATCTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACg < 2:58172/250‑1 (MQ=255) aTTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCtt < 1:225754/225‑1 (MQ=255) aTTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGAGAAGGAGTGAAAGATTTCCACTTCTATACGCtt > 2:225754/1‑225 (MQ=255) tGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGAtt < 2:105122/250‑1 (MQ=255) tattCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCAGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGCCGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTACACTTCTATACGCTTAACCGTGCTGAAATGAGATACGAGATTTGCCATACGCTgg > 2:284559/1‑251 (MQ=255) cgttATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCATCGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGTAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGTTTAGACCTGGTTta < 2:271379/248‑1 (MQ=255) tt‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTTGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAgtgt < 2:143302/249‑1 (MQ=255) gaagaaATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAACTGAGTTACGCGATTTGCCATACGCTGGGGGTTCGCCCTGGTTTATAATTAGTGTGGCTTTTGTGa > 1:2768/1‑250 (MQ=255) gaaATTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAAt > 1:222986/1‑251 (MQ=255) aaTTTGCCGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATc > 1:64012/1‑250 (MQ=255) ccGATATGACCAACGTGCGTATTCCAGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATCACACAgg > 1:254313/1‑250 (MQ=255) | ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAACTTT‑AAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGCAAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACTGGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCTGGTTTATAAATAGTGTGGCTTTTGTGAAAATCACACAGT > NC_000913/4133070‑4133536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |