Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,003,502	G→T	P226Q (CCG→CAG)	rarD ←	putative transporter RarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,003,502	0	G	T	94.4%	40.9 / NA	18	P226Q (CCG→CAG)	rarD	putative transporter RarD
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (6/11);  total (6/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTACCAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGT  >  NC_000913/4003258‑4003743                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   cGGCATATTTTTTCATAATATATTTATTTTATATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGctgtacgg                                                                                                                                                                                                                                              <  1:49845/251‑1 (MQ=255)         tttttCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAGTGAc                                                                                                                                                                                                                                       <  1:286849/250‑1 (MQ=255)         tttttCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTAAAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAGTGAc                                                                                                                                                                                                                                       <  2:74103/250‑1 (MQ=255)               aGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCACGTGGCAGCGGCGGTAAAACACAACAACTGTACGGGAGTGGCAATaacg                                                                                                                                                                                                                                >  1:78773/1‑248 (MQ=255)                          attttttatttGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCTGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAGTGACAATACCGGCGGCGATcag                                                                                                                                                                                                                     <  1:270423/251‑1 (MQ=255)                              tttatttGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAAAAAAACAGCTGTAAGGTAGGGGAAAAACCGGCGGCGATCAGCAGt                                                                                                                                                                                                                 >  1:182907/1‑251 (MQ=255)                                 atttGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCTCCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTaa                                                                                                                                                                                                               <  1:201753/250‑1 (MQ=255)                                         gTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAACTAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAATGACAATACCTgcggcgctccgccggaaaatcaacg                                                                                                                                                                                                       >  1:188597/1‑231 (MQ=255)                                                       ataAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGA‑CAGCGGGTTTTGc                                                                                                                                                                                        <  1:38554/250‑1 (MQ=255)                                                                 accttcACAAAAATTGCCACCGCCACCCAAATAAAGGCGAAAGTCCCCATCTTAGCGGCACCCGGTTTTGCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACTGTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCt                                                                                                                                                                             <  2:262664/247‑1 (MQ=255)                                                                               tGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCg                                                                                                                                                                <  1:97684/250‑1 (MQ=255)                                                                                                                                                    cccagccagcAGGAACATCAGCGGCGGGCCAATGTACTGTAAAAAGCCTAACGTTGTGAGCCGAAAGCGCGGGGCATCGGGGGTAAAACACAACAGCTGTAGGGTAGTGACAATTCCGGCGGCGATCAGCATTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCTGGTGGATCTGTCTGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTgcg                                                                                          <  2:236988/249‑1 (MQ=255)                                                                                                                                                       agccagcAGGAACTTCCGCGTCGGTCCAATGTACTGGAAAAAGCAGAACGGTGATAGACGCAAGGGCGTGGCAGGGGCGGTAAAACACAACAGCTGTACGTGAGTTACGAGACCGGTGGCGAGCAGCAGGAAATTCAGCGT‑GGTCGGGTTTTGTCCCAGATGTCGTGTTAAGGGGTCGGCAATAGCAAACAGGTAATTTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCTGTTTGCGCtt                                                                                       <  1:282770/251‑1 (MQ=255)                                                                                                                                                          cagcagGAAAATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCTAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATTCCGGCGGCGATCAGCAGTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGTCACATGGTTTCGATTAACATGCCGGTTTGCGCTTCaa                                                                                    <  2:78773/251‑1 (MQ=255)                                                                                                                                                                                             aaaaGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGAAAAAACCAAAAGCTGTAAGGAAGGGAAAAAACCCGCGGCGGTCATCAGTAAATTCAGAGAACA‑CAGGTTTTTCCACAAATAGCTTATTAAGCTTTCGGCAATAACAAAAAGGGAAATTGCCGCCACGGGCAGCAGCCACATGGGTTCGAGTAACATTCCGGATTGCGCCTCAACAGCActctctttaggtacccacgcgtagaaggc                                                  >  1:15824/1‑225 (MQ=255)                                                                                                                                                                                                 gCCTAACGTTGAGAGCCGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAGTGACAATTCCGGCGGCGATCAGCAGTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCTGGTTTAGCTGTCGGCAATAGCAAACAGGTAAATTGCCTCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTACCAGACCGTAGAAGGCAAAAc                                             <  2:182907/251‑1 (MQ=255)                                                                                                                                                                                                                                      gTAAACCACAACAGCTGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACAt                                                                                                                    <  2:59226/143‑1 (MQ=255)                                                                                                                                                                                                                                      gTAAAACACAACAGCTGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACAt                                                                                                                    >  1:59226/1‑143 (MQ=255)                                                                                                                                                                                                                                            cacaACAGCTGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTACCAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGt  >  1:116147/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   CGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGA‑CATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTACCAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGT  >  NC_000913/4003258‑4003743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑