Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,926,9910GA94.7% 50.6 / ‑3.6 19L5L (CTG→TTG) asnCDNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (8/10);  total (8/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

GGCGCTCTTTAA‑TATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACA‑TCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTT  >  NC_000913/3926767‑3927210
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                           
gggcgtctTTAAATAAAATGCCAATAAAGCCGCCTCCGTCATAACCGGGCTGCTTCGGG‑TGACAATCAATCCGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGTCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTa                                                                                                                                                                                                     <  2:76045/245‑1 (MQ=255)
 gtgcTCTTAAA‑TATAAGGCTGATAAAGCTGCCTACGTCAAAACCGAGTTGCTTCGGGCTAACA‑ACAATCCGCGCCCGGTTAATGACCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGc                                                                                                                                                                                                  <  2:248411/249‑1 (MQ=255)
 gcgcTCTTTAA‑TATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACA‑TCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGc                                                                                                                                                                                                  <  1:221440/251‑1 (MQ=255)
                   gccaaaaatgaatccgaagTCATAACCGTGCTGCTTGGGGGAGACA‑TCAAAAGGCCCCCCGGTAAGGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGTCTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGTTGCGCGCATTGCCCATTAATGTTTCCAGGATGTCACGGGCCAGATTGTCGATCAAATAATTTTCCATTGGATTTTCTTATGCGGATtgatgagtcttt                                                                                                                                                                                  <  2:246389/232‑7 (MQ=255)
                         aaaGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACA‑TCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGCATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTAtttt                                                                                                                                                                           >  2:94496/1‑251 (MQ=255)
                                           aCCGAGCTGCTTCGGGCTGACA‑TCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATg                                                                                                                                                          >  1:294503/1‑250 (MQ=255)
                                                                                                  cTTCATTTTCTCTACTCGTACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGGGCGCGCATTGCCCATTAATGCTTCCAGGATGCCCCGGTCCAGATTGTCGATCAAATAATTTTCCATAGGCTTTTCTTATGCGGCTTGATGattc                                                                                                                                                                                     >  2:52133/1‑169 (MQ=255)
                                                                                                  cTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCGTATGCGGATTGATGattc                                                                                                                                                                                     <  1:52133/169‑1 (MQ=255)
                                                                                                                  cGAACGTGAAACGTTCCCGGACTGACGCCAAATTGTTTCGCCAGTCCGGCGTAAGCGGTGTGCGCTTTTCCCATTAATGCTTCCAGGAGGTCACGGTTATGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATGCATTCTATTTTAGCCTTCTTTTTTAATGAATCAATAGTGAGTTAGGCTTTATATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTACTCagaa                                                                                   <  2:213507/251‑4 (MQ=255)
                                                                                                                                  ccGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATACGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAATAGGACGCAgg                                                                    >  1:176629/1‑250 (MQ=255)
                                                                                                                                   cGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGCTGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGcggg                                                                    >  2:95615/1‑246 (MQ=255)
                                                                                                                                                        cGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCtt                                             <  1:94496/251‑1 (MQ=255)
                                                                                                                                                               tCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTg                                       <  1:109093/250‑1 (MQ=255)
                                                                                                                                                                 ggCGTCAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATaaa                                                            >  2:280853/1‑227 (MQ=255)
                                                                                                                                                                 ggCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATaaa                                                            <  1:280853/227‑1 (MQ=255)
                                                                                                                                                                             gcgcgcATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCaaa                        <  1:69593/251‑1 (MQ=255)
                                                                                                                                                                                    ttGCCCATTAATGCTTCCAGGATGCCACGGACCAGATTGTCGATAAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCtt                 >  1:226804/1‑251 (MQ=255)
                                                                                                                                                                                              aTGCTTCCAGTATGCCACGGACCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCATTCTTTTTTCATGAATCAAAAGTGATTTAGGCTTTTTATTGACTGATTCTTGCATGAGTGTCGGTTTTTGTTGCTTAATCATACGCACCAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATAGCCAAACACCGTCAAATTAGCTTCGTGAAAtct       >  2:180053/1‑251 (MQ=255)
                                                                                                                                                                                                    ccatgATGCCTCTGTCCAGATTTTCGATCAAATAATTTTCCATAGGTTTTTCTTATGCGGATTTATGAGTCATTCTATTTTTGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATTATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCTGGAAATCTCACtt  <  2:169633/246‑1 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                           
GGCGCTCTTTAA‑TATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACA‑TCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTT  >  NC_000913/3926767‑3927210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑