| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,773,176 | C→T | S299F (TCT→TTT) | mtlA → | mannitol‑specific PTS enzyme IICBA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,773,176 | 0 | C | T | 97.3% | 102.3 / ‑4.2 | 38 | S299F (TCT→TTT) | mtlA | mannitol‑specific PTS enzyme IICBA component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (14/22); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01 | |||||||||||
GCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGTAAAATCATC > NC_000913/3772935‑3773423 | gCTACCCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACAGGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCt < 2:85550/251‑1 (MQ=255) aggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTTTACTTGCGATGac > 1:208587/1‑251 (MQ=255) aggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGGTCACGCCGCCTCACCTGGGCGGGGGTCTGGGTTCCCCGGCCTC‑CCCGGTTTTATTCTTGCttgtctggcgtagac > 2:4776/1‑237 (MQ=255) gctgGCGTACATGTTCTTTGGTCGTTGTAGCGCGAAACAGTCTGCTGGCGGTGCGGCAATCATTCCCTCCCTGTGTGGTATCCACGAAATCTCCTTCCCGTATGTGCTGATGAATCCTCGTCTGATCCTCGCAGTCATCCTCGGCTGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCt < 2:209295/249‑1 (MQ=255) ggCGTACATGTTCTTTGGTCGTGGGAGCGTTCAACAGTCCGCGGGCGGGGCGGCAATCATCCACTTCCTGGTGGGTATCCTCGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTTTGATCCTCGCAGTCATCCTCGGCGGTATGATTTGCGTGTTCTCGCTGTCTTTCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTC‑TTGTTTTAGCCTTGCTGAACTGGCGATGTCACCAAAAGGTGCTTACt < 2:98618/250‑1 (MQ=255) gattccatgtttgtgggtcttcgTAGCGCTAGGCAGTCTGCGGGCGGTGCGGCTATCATCCACTGCCCGGGTGGTTTCCACGAAATCTACTTCCCGTATGTGCGGATGAAGCCGTGTCGGATCCTCGCAGTCACCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTATGGTTTCTCCGGCATCTCCGG‑TTTTAGCCTTGCTGTCCTGGCGATGACACCAAAAGGAGCTTACTTc < 2:164135/229‑1 (MQ=255) cATGTTCTTTGGTCGTGGTATCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTaa < 1:188305/251‑1 (MQ=255) tGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCAACTTCCTAGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATACTCGCAGTAATCCTCGGCGGTATGACTGGCGTGATCACGCTGAATATCCTGGGCGGTGGTCTGGTTTCTACCGCATCTCCGG‑TTTTATCCTAGCTGTACTGGCGATGACCCCAAAAGGTGCTTACTTCGCTAACCTCGCggg > 1:285235/1‑251 (MQ=255) cGTGGTAGCGCTAAACAGTCTGCGGTCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTGCCCGTATGTTCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGTTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtgt‑g < 1:292720/251‑1 (MQ=255) cGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGgtgt‑g < 1:119337/251‑1 (MQ=255) atcAGTCTGCGGGCGGTACGGCAATCATCCACTTACTGGGGGGTCTCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCTGTATGACTGGCGTGTGCACGCTGACTATCCTGGGCTGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACTAAAAGGTGCTTACTTCGCTTACATCGCGGGTGT‑GTG‑TGCGGCGATgg < 1:257526/249‑1 (MQ=255) aaCATTCTGCGGGCGGTGCGGCTATCATCCCCTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAAGCCGCGTCTGATCCTCGCATTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATgg < 2:225379/251‑1 (MQ=255) cAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCt < 1:189785/251‑1 (MQ=255) ggTGCGGCAATCATCCCCTTCCTGTGGGGTATCCACGAAATCTCCTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGGTCACGCTTACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTAGCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGtt < 1:12249/251‑1 (MQ=255) gCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGGTGGCTGTCTCCTTCGTTggt > 1:209543/1‑249 (MQ=255) cGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGCATCCGCGTCTGATCCTCGCAGTCATCATCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGGGGTCTGGTTTCTACGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTATCCTTCGTTGtct > 1:43523/1‑251 (MQ=255) ggCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGGGCTTACTTCGCTAACATCGCGGGTG‑‑GTGGTGCGGCGGTGGCTGTCTCCTTCGTTGtctc > 2:83726/1‑251 (MQ=255) catcCACTTCCTGGGGGTGATCCACGAAATCTACTTCCCGTTGGGGCTGACGAATCCGCGTCTGATCCGCGCAGTCACCCGCGGCGGTATGACTGGCGTGTTCACTCTGACTACCCTGGGCGGTGGTCTGGGTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCGAAAGGTGCTTACTTCGCTATCATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCGTCGTTGTCTCTGCTa < 2:73640/250‑1 (MQ=255) catcCACTTCCTGGGGGGTATCCACGAAATCTCCTTCCCGTATGTTCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTAGCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTa < 2:16295/250‑1 (MQ=255) ccACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAAGCCGCGTCTGAGCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTAttt < 2:132265/250‑1 (MQ=255) ggggTATCCACGAAAGCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTAATCCTCGGCGGTATGCCAGACGTGTTCACGCTGACTATACTGGGCGGTGGTCTGGTTTCTCAGGCATCTCCGG‑TTTTATACTTGCTGTACTGGCGGTGACAACAAAAGGTGCGTGCTTAGCTAACATCGCGG‑TGTTGTG‑GTCGGCGGGGGCTGTCTCCTTCGTTGTCTCTGCTCTTTTTCGGAAAAca > 2:173008/1‑249 (MQ=255) ggTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGc > 1:237470/1‑251 (MQ=255) aaaTCTACTTCCCGTATGTGCTGCTGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGGCTTGCGTGTTCACTCTGACTATCCTGGGCGGTGGGCTGGTTTCTCCGGCCTCTCCGG‑TTTTTTCCTTGCGATACTGGCGATTACACCAAAAGGGGCGTAATTCGCTAACCTCGCGGGTGT‑GTG‑TGAGGCGGTGGCTGGATCCTTCATTGTCACTGCTCTTTTGTTGGGAACCAGCAAAgggga > 2:246239/1‑245 (MQ=255) cccGTTTGTGATTATGCATCCGCGGTTGATCCTCGCGTTCAGCCTCGTCGGTATTACTGGCGTGTTTACGCTGACTATCCTGGGCTGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTTCTTCGCTAACATCGCGGGTTT‑GTG‑TGCGGCGATGGCTGTCTCATTCGTTGTCTCTTCTATTTTGCTGAACACCAGCAAAGTGAAAGAAGAAgatg < 2:163705/250‑1 (MQ=255) gTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGatat > 1:273279/1‑251 (MQ=255) aTGTGCTGATGAATCCGCGGCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCAAGCTGACTATCCTGGGCGGTGGTCTGGGTTCTCCGGCATC‑CACGGTTTTATCCTTGCTGGACTGGCGATGAAAACAAAAGGGGCTTACTTCGCTAACATTGCGGGAGT‑GTG‑TGCGGCGAGGGCTGTCTCCTTTGTTGTATCTGCTATTTTGCTGCGAAACAGCAAAGAGAAAGAAGGAGATGATATTg > 1:66236/1‑251 (MQ=255) aaTCCGCGTCTGATCCTCGCAGTCATACTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTT‑TATCCTTGCTGTACTGGCGATGACAACAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTTTGTCCTTCGTTTTCTCTGCTTTTTTGCTGAAAACCAGCCAAGTGAAAGAAGAAGCTGGTATTGAATAAGCAAc > 2:272809/1‑250 (MQ=255) aTCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGCCTATCCTGGGCGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGGTTCTGCTATTTTGCTGGAAACCCTCAAAGTGAAAGAAGAAGATGAGATTGAAGCAGCAACtc > 2:161575/1‑251 (MQ=255) gcgTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACtcgtcg < 1:83726/251‑1 (MQ=255) cccggcggTATTTCTGTCGTGTTCACGCTGACTATCCTGGGCGTTGGTCTGGTTTCTCCGGCAGCGCCGG‑TTTTATCCTTGCTGTACTGGCGATGGCACCAAAATGTGCTTACGTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATTGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTTAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCttag < 2:251279/249‑4 (MQ=255) cggcggTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTAGCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGt < 1:240433/250‑1 (MQ=255) aCTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGc < 1:191656/251‑1 (MQ=255) aTCCTGGGCGGTGGTCTGTTTTCCCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACTCCAAAAGGTGCTTTCTTCGCTAACAGCGCGGGTGG‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGAt < 2:171163/251‑1 (MQ=255) gCGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGACAACCAGCTAAGTGAAGGTAGAAGATGATATTGAAGCAGCAACTAGACGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCCTCTCCGCTATCTGCAGGCGATGTGACt > 2:154326/1‑250 (MQ=255) tCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGCTGATATTGAAGCAGCAACTCGTCGTATGCAGGAAATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCt > 1:177753/1‑251 (MQ=255) ggttttcccggcttcaccggTTTTATCCTTGCTGTACGGGCGAGGACATCAAAAGGTGCTTACGTCGCTAACTTCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTGCTTCTTTGTCTCCGCTATTCTGCTGAAAACCATCAAAGTGAAAGAAGAAGATGATAGTTAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCGGGCTGCTGGCGATGTGACTAACGACCTGAg < 2:102231/233‑1 (MQ=255) ggCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCacgtacg < 1:165074/251‑1 (MQ=255) ttCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCAATGTGACTAACGACCTGAGCCACGTACGTAAAatcatc > 1:21748/1‑250 (MQ=255) | GCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGT‑GTG‑TGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACCTGAGCCACGTACGTAAAATCATC > NC_000913/3772935‑3773423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |