Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,423,8250CT28.1% 40.1 / 17.0 34P42P (CCC→CCT) ulaFL‑ribulose‑5‑phosphate 4‑epimerase UlaF
Reads supporting (aligned to +/- strand):  ref base C (9/14);  new base T (5/4);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAG‑GCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTT  >  NC_000913/4423580‑4424061
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             
cGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAgcgg                                                                                                                                                                                                                                          >  1:196971/1‑251 (MQ=255)
         ccTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCTTAGCGGCGTTGCCt                                                                                                                                                                                                                                  >  1:33438/1‑250 (MQ=255)
                gaggtgtgGAGAGAAACGGCGTAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGGCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAAc                                                                                                                                                                                                                           <  2:234029/246‑1 (MQ=255)
                     gtgGAGCGAAACGGCGGGAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGC‑GCGCATGGCGAAAGAGGGCATGGGGGCGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGCCCGCGAGCGCAGGCTGGGGGTGATAAAGCCCAGCGGAGTTGCCTACGCAAccgcga                                                                                                                                                                                                                      >  2:230419/1‑246 (MQ=255)
                        atcgtaaaCGGCGGAAGACCCTGCGGCAGAAGTGGCGAAAGCGCGTGGTTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCACAGCGGCGTTGCCTACGAAACCATGAAAGc                                                                                                                                                                                                                  <  2:166740/246‑1 (MQ=255)
                            gAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGCACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGc                                                                                                                                                                                                                  >  1:166740/1‑247 (MQ=255)
                                 ggcggAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCTTCGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGatat                                                                                                                                                                                                         >  2:125383/1‑251 (MQ=255)
                                     gAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGAGTGGGTGAAAGCTGC‑CATGGGGAGAGCGGGAGTGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACAGGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATAtggtg                                                                                                                                                                                                     <  2:96278/251‑1 (MQ=255)
                                          cccGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGG‑GCGCATGGCGAAAGCGGGGATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCGTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATggtggtggt                                                                                                                                                                                                <  2:104421/251‑1 (MQ=255)
                                                ggcAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGC‑GAGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCCGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCTTAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGatat                                                                                                                                                                                          <  2:86260/251‑1 (MQ=255)
                                                               aGCGCGTGATTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAggtggt                                                                                                                                                                           <  1:247329/251‑1 (MQ=255)
                                                                   cgTGATTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGaa                                                                                                                                                                       <  1:172335/251‑1 (MQ=255)
                                                                       gttGGTTGAAAGC‑GCGCATGGCGAAAGCGGGAATGGGTGAGGCGGCATAATGCAAAAGCTAACACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTAGCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCATACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGTGGGTGGAAgggg                                                                                                                                                                   <  2:213216/250‑1 (MQ=255)
                                                                             gtgaaggagcgcATGGAGAAGGCGGGCATGGGGGAGGCGGTATAATGCAAAAGCTAAAACAGAAGGTAGTTGATGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCTACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGTCGTTGCCTACGAAACCATGAAAGCGGCCTATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTAt                                                                                                                                                              <  2:191504/243‑1 (MQ=255)
                                                                                                   cGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCg                                                                                                                                         >  1:214834/1‑250 (MQ=255)
                                                                                                         tggtggAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGCGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCTTAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGGGGAGGAAGGGGAGTATCGCCCCTCTTCCGACACTGAGACGCAtc                                                                                                                                  >  2:7207/1‑251 (MQ=255)
                                                                                                                  gcgccaaaggCAAAAGCTAAATCAGCAGTTTATTGAAGCCAACATGGAGCTTCCGCGCTACGGGCTGGTGACCTTTTCCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCTTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGATCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAActct                                                                                                                         <  2:218573/242‑1 (MQ=255)
                                                                                                                           gaaaaaGCTAAAACAGGCGGTATTTGATGCCAACATGGTGCTGCCGCGCTACGGGCTGGTGTCCTTTCCCTGGTGCAACGTCAGCGTTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCATCGGCGTTGTCTACGAAACCATTAAAGCGGCCGATATGGTGTTGGTTTATATGAGCGGCAAGGTGGTCGAAGGTGAGTAGCGCCTATCTTCCGACACTGAGACGCATCTCGAACTCTACcgtcgtg                                                                                                                <  2:203354/249‑2 (MQ=255)
                                                                                                                                        cagcagGTATTGGAAGTCAACATGGAGCTTCCGCGCTACGGGCTGGTGACCTTTCCCTGGGGCATCGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTtgg                                                                                                   <  1:276321/251‑1 (MQ=255)
                                                                                                                                                   tGAAGCCAACATGGAGCTGCCGCGCTTCGGGCTGTTGACCTTTCCCGGGGGCAACGGCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCTTAGCGGCGTTGCCTTCGAAATCATGAAAGCGGCCAATATGGTGGCGGTTGATATGAGCGGCAAGGTGGTGGAAGGTGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTc                                                                                         <  2:33438/250‑1 (MQ=255)
                                                                                                                                                     aaGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCAt                                                                                      >  1:77092/1‑251 (MQ=255)
                                                                                                                                                                    tGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCcaccccactcct                                                                       >  1:250642/1‑249 (MQ=255)
                                                                                                                                                                       cgcgCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGAGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGGGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGAATCTCCCATCTTCCGACACTGCGACGCATATCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCCCTCCACTCATGcc                                                                    >  1:205392/1‑251 (MQ=255)
                                                                                                                                                                                     gttgccgttagcTGGGGCAAAGTCAGCGCTATCGACCGCTAACGCGCGTTGGTGGTGATCGATCCCAGTGGCGTTGCCTACGAAACCTTGAAAGCGGCCGATATGGTGGTGTTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACTCTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCGTGGTGGCATTGTCCATACCCACTCCACTCATGCCCCCGCATGGGCGCa                                                      <  2:163594/240‑1 (MQ=255)
                                                                                                                                                                                            ttACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCTTAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCAAGCCACCGCATGGGCGCAG‑GCggg                                               <  1:125383/250‑1 (MQ=255)
                                                                                                                                                                                             tACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAG‑GCggggg                                             >  1:128241/1‑250 (MQ=255)
                                                                                                                                                                                                cTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGATGGTGGTGATCAAGCCAAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGCTATGAGCGGCAAGGAGGTGGGCGGGGAGGATCGCCCATCTTCCGACCCTGCGCCGCATCTCGAACTCTACCGTCGGTCCCCGTCGCTTGGTGGCCATGTCCATACCCACTCCACTAATGCCACCGCCAGGGCGCGG‑GCgggggt                                            >  2:190123/1‑247 (MQ=255)
                                                                                                                                                                                                            cAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGCTCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGCGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGCGTATCGCCCATCTTCCGACACTGCGACGCAt                                                                                                                                   >  2:199983/1‑151 (MQ=255)
                                                                                                                                                                                                            cAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCAt                                                                                                                                   <  1:199983/151‑1 (MQ=255)
                                                                                                                                                                                                                          gcgAACGCGGGCTGGTGGGGAGAAAGCCAGGCGGCGTTGCCTACGAAACCATGAAAGCGGTCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACGCCACTCATGCCACCGCATGGGCGCAG‑GCGGGGCTGGCGATCCCGGCGTTAGGCTCcactcac                <  2:234223/251‑1 (MQ=255)
                                                                                                                                                                                                                                  gggCTGGGGGTGATCAAGCGTAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGGGGTGGTTGATATGAGAGGCAAGGAGGAGGAAGGGGAGAATCGACCATCTTCCGACACTGCGACGCATCGGGAACTCTACCGTCGTTACCCGTCGCTTGGGGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCG‑AGAGCGGGGGTGGAGAGCCCCGCGGTAGGACCCACACACGCCgcac         >  2:191768/1‑247 (MQ=255)
                                                                                                                                                                                                                                        gtggtgATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAG‑GCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTActtctt  <  1:190123/251‑1 (MQ=255)
                                                                                                                                                                                                                                            tgATCAAGCTTAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGAc                                                                                                                                       <  1:200508/115‑1 (MQ=255)
                                                                                                                                                                                                                                            tgATCAAGCTTAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCACATCTTCCGACACTGCGAc                                                                                                                                       >  2:200508/1‑115 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             
CGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGC‑GCGCATGGCGAAAGCGGGCATGGTGGAGGCGGCATAATGCAAAAGCTAAAACAGCAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAG‑GCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTT  >  NC_000913/4423580‑4424061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑