Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,000,5940CA21.7% 86.0 / 16.8 46A155S (GCG→TCG) ‡yigEDUF2233 domain‑containing protein YigE
Reads supporting (aligned to +/- strand):  ref base C (17/19);  new base A (4/6);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCAAGATAAAGCAGCTGCTCAACGTTCAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTA  >  NC_000913/4000350‑4000842
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        
cccccGCCTTTCATATACATATGTTAAATCGTGCCATCAAGATAAAGCAGCTGCTCAACGTTCAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTg                                                                                                                                                                                                                                                    <  2:107376/250‑1 (MQ=255)
 ccccGCCTTTCATATACATATGTGAAATCGTGCCATCAAGATAAAGCAGCTGCTCAACGTTCAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGCGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTg                                                                                                                                                                                                                                                    >  2:164522/1‑250 (MQ=255)
                aCATATGTGAAATCGTGCCATCAAGATAAAGCAGCTGCTCAACGTTCAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGtt                                                                                                                                                                                                                                    <  1:94047/251‑1 (MQ=255)
                 cATATTTGAAAGCGTGCTATCAATATAAAGCAGCTGCTCAACGTCCAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAATTGTGTTTACTGCTGGCTCAACAAAAACACGTCGTTCCCATGTTTATTAAGTCCAACACCGTTACGAATTTTGCGTGAGGCGACGTTGGGATGAATACTCGGATTAATTACACCGTGTTCCATCTTCATTGGCCCTGACTGCACCTCAAACTGATTCTCTTTACTGGttt                                                                                                                                                                                                                                   <  2:19713/251‑1 (MQ=255)
                             cGTGCCATCAAGATAAAGCAGCTGCTCAACGTTCAGTTTCGCTTTGTCATAACAGGCAAAATCATAAAAATTTGTTTCCTGCTGGCTCAACAAAAACCCGGCGCTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACTCCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATcc                                                                                                                                                                                                                        <  2:2196/250‑1 (MQ=255)
                              gTGCCATCAAGATAAAGCAGCTGCTCAACGTTCAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACC‑TTTTCCATCAACATTGGCCCTGACTGCACAGAAAACTGAATCTCTTTACTGGTTTTGAAGGCATcc                                                                                                                                                                                                                        >  1:104786/1‑248 (MQ=255)
                                      aaGATAAAGCAGCTGCTCAACGTTCAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGacaaacg                                                                                                                                                                                                               >  2:37126/1‑250 (MQ=255)
                                              gcagcTGCTCAACGTTCAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCAAGACGAACGATGCCGACt                                                                                                                                                                                                      >  1:34643/1‑251 (MQ=255)
                                                              cAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACAt                                                                                                                                                                                      <  1:164522/251‑1 (MQ=255)
                                                                      cTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACAGAAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACAcg                                                                                                                                                                              >  2:84938/1‑251 (MQ=255)
                                                                              aaaCAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTATGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAgg                                                                                                                                                                      <  1:238261/250‑1 (MQ=255)
                                                                                          tCATAAAAATTTGTTGCCTGCTGGCTCAACAAACACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTACTTACACCGTTTTCCATCAACATTGGCCCTGACTGCACAGAAAACTGACTCTCTTTACTg                                                                                                                                                                                                                                       >  2:46486/1‑174 (MQ=255)
                                                                                          tCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGAGTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACAGAAAACTGAATCTCTTTACTg                                                                                                                                                                                                                                       <  1:46486/174‑1 (MQ=255)
                                                                                             tcaaaaTTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGacg                                                                                                                                                                                                                   >  2:161409/3‑191 (MQ=255)
                                                                                             tAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGacg                                                                                                                                                                                                                   <  1:161409/191‑1 (MQ=255)
                                                                                                                                  cGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTAATGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAAAACGCCGCCAGGACGGCTAAAGAAATTCACTTCACCTGAAGCGAGATTTAACGCCAACTTctgc                                                                                                                  >  1:273355/1‑251 (MQ=255)
                                                                                                                                              tattaATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTc                                                                                                       <  1:59216/250‑1 (MQ=255)
                                                                                                                                                 taATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGACTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATg                                                                                                   >  2:188746/1‑251 (MQ=255)
                                                                                                                                                   aTCCCAACACCGATACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTATTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGt                                                                                                  >  2:20286/1‑250 (MQ=255)
                                                                                                                                                           acCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACC‑TTTTCCATCAACATTGGCCCTGACTGCACAGAAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAg                                                                                        <  2:104786/251‑1 (MQ=255)
                                                                                                                                                                      ttttGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACAGAAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAcc                                                                                                                                              >  2:41309/1‑187 (MQ=255)
                                                                                                                                                                      ttttGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACAGAAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAcc                                                                                                                                              <  1:41309/187‑1 (MQ=255)
                                                                                                                                                                         tGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCtt                                                                           >  1:113099/1‑251 (MQ=255)
                                                                                                                                                                                 gCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTTCAAACCGAGCGGCGCATAGCTTTCATCATa                                                                   <  1:231626/251‑1 (MQ=255)
                                                                                                                                                                                       ttGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGCATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGCGATTTAACGCCAACTTCTGCTACCCGTTTTCGATGTTCAAACAGAACGGCGCATGGCTTTCATCATAGATgcc                                                             >  2:121006/1‑251 (MQ=255)
                                                                                                                                                                                          ggATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACAGAAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgccgcc                                                          <  1:84938/251‑1 (MQ=255)
                                                                                                                                                                                            aTGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGTCATCCCGACGAAAGCTGCCGACTTTATCTACCGCGACATAACACACGCCGCCAGGACGGCTAAAGAAGTTCCCTTCACCTGAAGCGGGATTTAACGCCCCCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCCTCGCTTtcatagtgcggaatagcat                                                        >  2:159011/1‑236 (MQ=255)
                                                                                                                                                                                            aTGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGCCCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGt                                                        >  1:262544/1‑251 (MQ=255)
                                                                                                                                                                                            aTGAATACGCGGATTAATTACACCGTTTTCCATCAACATGGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCCCCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcat                                                                       <  1:159011/236‑1 (MQ=255)
                                                                                                                                                                                              gAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCTCATAGCTTTCATCATAGATGCCGCCGTTc                                                      <  1:2967/251‑1 (MQ=255)
                                                                                                                                                                                                    gcgGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCACACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATCGATGCCGCCGTTCATCGcc                                                >  1:268647/1‑251 (MQ=255)
                                                                                                                                                                                                        attaattaCACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCACCGTTCATCGCCATCt                                            >  1:164877/1‑251 (MQ=255)
                                                                                                                                                                                                          ttattaCACCGTTTCCCATCAACATTGGCCCTGACTGTACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGTCTTTATCTCCCGCGCCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTg                                           <  2:164715/248‑1 (MQ=255)
                                                                                                                                                                                                          taattaCACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTATTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGACATCTGc                                          >  1:186115/1‑251 (MQ=255)
                                                                                                                                                                                                                cacCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctg                                     >  1:196087/1‑250 (MQ=255)
                                                                                                                                                                                                                  ccGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaac                                  <  1:214735/251‑3 (MQ=255)
                                                                                                                                                                                                                         ccATCAACATTGGCCCTGACTGCGCAGAAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACt                            <  1:144671/250‑1 (MQ=255)
                                                                                                                                                                                                                         ccATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGAGGCCGACTTTATCTCCCTCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTa                           <  1:207944/251‑1 (MQ=255)
                                                                                                                                                                                                                            tCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGAACCTGACCCTGACTAtt                         >  1:252302/1‑250 (MQ=255)
                                                                                                                                                                                                                            tCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCAATGTACAAACCGAGCGGCGCATAGCTTTCATCATAAATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGAATAtt                         >  1:204376/1‑250 (MQ=255)
                                                                                                                                                                                                                             cAACATTGGCCCTGCCGTCACCGCAAACTGACTCTCTTTACTTGTTTTGAAGGCAGCCAGACTAACCATGCCGTCTTTATCTCCCCCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCCCCTGAAGCGATATTTAACGCCACCGTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGTCCCTGACGATTa                        <  2:232681/250‑1 (MQ=255)
                                                                                                                                                                                                                               aCATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAata                     <  1:110038/251‑1 (MQ=255)
                                                                                                                                                                                                                                      acctcaCTGCACCTCAAACTGACGCTTTTGACTTGCTTTGAAGACATCCAGACTAACGATGCCGGCTTTCTCTCCCGCTACATAAAACCCTCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCGCCTTCCGCTGACCGTGTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGccc              <  2:2791/246‑2 (MQ=255)
                                                                                                                                                                                                                                        cggattGCACCGAAAACGTAATCTTGTTACTGGCTTTGAAGGCAACCAGACGCAAGATGCCGTCTTTATCCCCCGCGACATAAAACACGCCGCCAGGACGTATAAAGAAATTCCCTCCACCTAAAGCTAGATTTAACGCCACCTTCTGCTGACCGTCTTCGATGTACAATCCGAGCGGCGCATCTCTTTCATCATAGATGCCGCCGTCCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagc            <  2:133940/246‑1 (MQ=255)
                                                                                                                                                                                                                                              gCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCa      <  1:188746/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTa  <  1:20286/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        
GCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCAAGATAAAGCAGCTGCTCAACGTTCAGTTTCGCTTTGGCATAACAGGCAAAATCATAAAAATTTGTTGCCTGCTGGCTCAACAAAAACACGGCGTTCCCATGTTTATTAATCCCAACACCGTTACGAATTTTGCTTGAGGCGACGTTGGGATGAATACGCGGATTAATTACACCGTTTTCCATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTA  >  NC_000913/4000350‑4000842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑