Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,474,2720CT73.1% 32.6 / 10.8 27R94H (CGT→CAT) rpsL30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base T (12/7);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCAC‑GTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCG  >  NC_000913/3474023‑3474513
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
gaaccGAATTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAAc                                                                                                                                                                                                                                                    <  1:116223/247‑1 (MQ=255)
            ggATCCAGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCggg                                                                                                                                                                                                                                         >  1:83392/1‑249 (MQ=255)
               tCCGGCAGAATTTTACGCTTACCAATGACGTGACTACGTGGCATGTAAATACTCCGTGGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTGGACATGTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGTCGCGTCACGCCATACTTGGAACGAGCCTGTTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTTGTAATGAACACCCGGGAGGt                                                                                                                                                                                                                                     <  2:293344/250‑1 (MQ=255)
                                      aaTGACGCGACGACGTGGCATGGAAATACTCCGTTGTTTATTCAGGATTGACCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAATGAACACCCGGGAGGTCTTTCACACGACAGCCCCgggta                                                                                                                                                                                                              >  2:44127/1‑247 (MQ=255)
                                                 gacgTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCCCGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTACCACGACCGCCCCGGATCAGGATCACgg                                                                                                                                                                                                    >  2:143572/1‑249 (MQ=255)
                                                                                   gATTGTCCAAAACTCGACGATTTTAGTTTGTCATATAAGTTAAAACGTGTGGCTTTATTTAACGGAGACGCATTAAGCTTTAGGACGCTTCTCGCCATTCTTGGAACGGTCCGGCTTACGGTCTTTAATGTCGTGGCGGTCAAGCGCACTCCGCACGTTGTGGTAATGAACACCCGGAAGTTCTTTACCGTGACCTCCTCGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCTCCACCGATGt                                                                                                                                                                <  1:145194/251‑1 (MQ=255)
                                                                                       gTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAATGAACACCCGGGAGGTCTTTTACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAgg                                                                                                                                                             >  2:265735/1‑250 (MQ=255)
                                                                                          cAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTGCGGTGTGGTAATGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAgg                                                                                                                                                         >  2:165813/1‑250 (MQ=255)
                                                                                                    cGAGTTTAGTTGGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACGTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATCTAGGAAGTCACTTCGaaa                                                                                                                                               <  1:143572/251‑1 (MQ=255)
                                                                                                      agtttagtttGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAATGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAAcc                                                                                                                                             >  1:244061/1‑251 (MQ=255)
                                                                                                         ttagtttGCCATTGAAGTAAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGTACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCTCCCCGTACGTTGTGGTAATGAACTCCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCTATGTAGGAAGTCACTTCGAAACCGt                                                                                                                                           <  1:293811/250‑1 (MQ=255)
                                                                                                                gaaaTTTACGTTAAAACGTTTGACCTTACTTAACGGAGAACCATTAAGACTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCGTTAACGCCGGACCAGTCAGGCACACCAAGTACGGTGAGGTAATGAACACCCGGTAGGACTTTAACACGACCGCCACGGATCAGGAACACGGAGTGCGACAGCAGATTGTGACCTTCACCACCAATGTAGGAAGTCACTTCGAAACCGTTAGTc                                                                                                                                      >  2:67429/4‑248 (MQ=255)
                                                                                                                        aGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAAAGAGCCTGCTTACGGTATTTACCGCCGGAGCAGTCAAGCGCACCAAGTACGGTGTGGTAATGAACACCCGGGACGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTACGACACCGTTAGTAAGACGAGCACg                                                                                                                           >  1:200314/1‑251 (MQ=255)
                                                                                                                            aaaaCGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGAAATGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCATGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGCAACCGTTAGTCCGTCGAACACAGCAt                                                                                                                       >  1:234366/1‑251 (MQ=255)
                                                                                                                             aaaCGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAATGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCAt                                                                                                                       >  1:230679/1‑250 (MQ=255)
                                                                                                                                 gTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCGGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATCTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACttt                                                                                                                  >  2:2647/1‑251 (MQ=255)
                                                                                                                                         ttacttaACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAATGAACACCCGGGAGGTCTTTAACACGACCGCCACGGCTCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAgcg                                                                                                          >  1:202858/1‑251 (MQ=255)
                                                                                                                                           acttaACGGAGACCCCTTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAATGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGcgcg                                                                                                        >  1:76522/1‑251 (MQ=255)
                                                                                                                                           acataACGGAGAACCATTAAGCCTTGGGCCGCTGAACGCCATCCTTGGACTGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATCTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACTGCATACTTTACGCAGcgcg                                                                                                        <  1:46634/248‑1 (MQ=255)
                                                                                                                                                              agctttgggACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCCCCACGTACGGTGTGGTAATGAACACCCGGGAGTTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAGCACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAg                                                                                     <  1:265735/244‑1 (MQ=255)
                                                                                                                                                                   tAGGACGCTTCACGCCATACTTGGAACGAGCCGGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATCTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGgt                                                                                 <  1:2647/250‑1 (MQ=255)
                                                                                                                                                                                     aCTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTCACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATCTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACAt                                                              >  1:265819/1‑251 (MQ=255)
                                                                                                                                                                                                          ggTCTTTAACGCCGGAGCAGTCAAGCTCCCCACGATCGGTGTGGTAATGAACCCCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCAC‑GTTTTTGCGGGCAt                                        <  1:158252/251‑1 (MQ=255)
                                                                                                                                                                                                                   cGCCGGAGCAGCCAAGCGCACCCCGTACGGTGTGGTAAGGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCAC‑GTTTTTGCGGGCATGCTTCCAgc                               <  1:216614/251‑1 (MQ=255)
                                                                                                                                                                                                                      cGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAATGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCAC‑GTTTTTGCGGGCATGCTTCCAGCGCa                            <  1:23243/251‑1 (MQ=255)
                                                                                                                                                                                                                         aGCAGTCAAGCGCACCACGTACGGTGTGGTAATGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCCCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGCAACCGTTAGTCAGTAGAACACGGCCTACTTTACGCAGCGCGGAGTTCGGTTTGTTCGGAGAGGTAGTATATACACGAGTACATACGC‑ACAGTTTTTGCGGGAATGCTTCCAGCGCAGGc                         >  2:105698/1‑251 (MQ=255)
                                                                                                                                                                                                                                             aCGGTGTGGTAATGAACTCCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCAC‑GTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACttt     <  1:245217/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  gtgGTAATGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCAC‑GTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCg  >  1:288331/1‑249 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
GATCCGAACTTCGGATCCGGCAGAATTTTACGCTGACCAATGACGCGACGACGTGGCATGGAAATACTCCGTTGTTAATTCAGGATTGTCCAAAACTCTACGAGTTTAGTTTGACATTTAAGTTAAAACGTTTGGCCTTACTTAACGGAGAACCATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCAC‑GTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCG  >  NC_000913/3474023‑3474513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑