Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,765,4111.G33.3% 0.1 / 11.0 9intergenic (‑258/+102)abpA/yfjManti‑bacteriophage protein/protein YfjM
Reads supporting (aligned to +/- strand):  ref base . (2/4);  new base G (3/0);  total (5/4)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATCAAGCTTTTCAGGATTCCACAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑G‑C‑‑‑‑‑‑‑ACTATGGCA‑‑‑‑‑‑‑‑CTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATCGCGTGTTCATGGCTTTCTATATTGTTGATGCCTTGCCCATCCGGACCCCACTCCTT  >  NC_000913/2765175‑2765623
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gCGAAAATTAAGGAGATGATATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATCAAGCTTTTCAGGATTCCACAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑g‑c‑‑‑‑‑‑‑actatggca‑‑‑‑‑‑‑‑ct                                                                                                                                                                                                         >  1:259926/1‑250 (MQ=255)
 cGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACAACATCATCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATCAAGCTTTTCAAGATTCCACAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACCTACTGCCCCAACTAACGTAAGGTAGGGACAGCCTTAATCACGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAAAC‑‑‑‑‑‑‑‑g‑c‑‑‑‑‑‑‑acactggca‑‑‑‑‑‑‑‑ctat                                                                                                                                                                                                       >  2:244201/1‑251 (MQ=255)
     aaTTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATCAAGCTTTTCAGGATTCCACAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑g‑c‑‑‑‑‑‑‑actatggca‑‑‑‑‑‑‑‑ctatggca                                                                                                                                                                                                   <  1:54487/251‑1 (MQ=255)
              gaTGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATCAAGCTTTTCAGGATTCCACAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑g‑c‑‑‑‑‑‑‑actatggca‑‑‑‑‑‑‑‑ctatggaactatgtcac                                                                                                                                                                                          >  1:214914/1‑251 (MQ=255)
                   tATGAAACAGTCCAATCGGATCACTGCATCCTCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGTTATCAAGCTTTTCAGGATTCAACAACTATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACAGCCAACC‑‑‑‑‑‑‑‑g‑c‑‑‑‑‑‑‑actatggca‑‑‑‑‑‑‑‑ctatggcactatggccctatgg                                                                                                                                                                                     >  2:284371/1‑251 (MQ=255)
                      gacaagttCCAATCGGATCACTCCATCATCACCAGTGATCTAAATCCTTCACACCCATTCTCGGTCGCAGGTATCAAGCTTGTCAGGATTCCAAAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑g‑c‑‑‑‑‑‑‑actatggca‑‑‑‑‑‑‑‑ttatggcactatggcactatggcac                                                                                                                                                                                  <  2:282815/244‑1 (MQ=255)
                               cAATCGGATCACTGCATCATCACAAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATCAAGCTTTTCAGGATTCCACAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑g‑c‑‑‑‑‑‑‑actatggga‑‑‑‑‑‑‑‑ctatggcactatggcactatggcaatatggcact                                                                                                                                                                         >  2:218404/1‑251 (MQ=255)
                                         aCTGCATCATCACCAGTGATCTAAATACTTCACAGCCATTTTCGGTCGCAGGTATCAAGCTTTTCAGGATTCCACAAATACTGTAACCCTGACGTCTGATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑G‑C‑‑‑‑‑‑‑ACTATGGCA‑‑‑‑‑‑‑‑CTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGc                                                                                                                                                               >  1:59335/1‑251 (MQ=255)
                                                                                                                                                         cATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACCGCACTATGG‑C‑‑‑‑‑‑‑ACTATGGCA‑‑‑‑‑‑‑‑CTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATcgcg                                                       >  1:10644/1‑251 (MQ=255)
                                                                                                                                                              tGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑G‑C‑‑‑‑‑‑‑ACTATGGCA‑‑‑‑‑‑‑‑CTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTAATATCTCGCTGCTCTTTGAGCAATGTc                                                                >  2:101205/1‑229 (MQ=255)
                                                                                                                                                              tGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑G‑C‑‑‑‑‑‑‑ACTATGGCA‑‑‑‑‑‑‑‑CTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTAATATCTCGATGCTCTTTGAGCAATGTc                                                                <  1:101205/229‑1 (MQ=255)
                                                                                                                                                               cccccaactacattAAGGAAGGCACAGCCTTAATCAAGGTTTGGTTTTTCAAATAGTCCAGAGCTGGGCCCGCCTACG‑‑‑‑‑‑‑‑C‑C‑‑‑‑‑‑‑CCGATGGCC‑‑‑‑‑‑‑‑CTATGGCACTATGGCACGATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATCGCGTGTTCATGGCTTTc                                         <  2:59335/238‑1 (MQ=255)
                                                                                                                                                                ccataacgaatgttaagaaatcataGCCTTAATCAAGGTTTTGGGTTACAGATAGTTGAGAGCAGAGACCGCCATCC‑‑‑‑‑‑‑‑GGCATATGGAACTATGTCA‑‑‑‑‑‑‑‑CTATGGCACTATTGCACCAGGGCTCTATGGCAGTATGGCTCCTCCAGCGATTATAATCACACTTGATATTATAAAACACAGTTGCACGCATAATTTCCTGGTTGGTAGTGTTATATCTCGATGCTCTTTGAGCAATGTCAGCATCGCGTGGTCAt                                                <  2:114924/227‑1 (MQ=255)
                                                                                                                                                                           gTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑G‑C‑‑‑‑‑‑‑ACTATGGCACTATGGCGCTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATCGCGCGTTCATGGCTTTCtata                                     <  1:195386/251‑1 (MQ=255)
                                                                                                                                                                                                           gttttCAAATAGTTCAGAGCTGAGACCGCCAACCGCACTATGG‑C‑‑‑‑‑‑‑ACTATGGCA‑‑‑‑‑‑‑‑CTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATCGCGTGTTCATGGCTTTCTATATTGTTGATGCCTTGCCCATCCGGACCCCACTc     >  1:30957/1‑251 (MQ=255)
                                                                                                                                                                                                              ttCAAATAGTTCAGAGCTGAGACCGCCAACCGCACTATGG‑C‑‑‑‑‑‑‑ACTATGGCA‑‑‑‑‑‑‑‑CTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATCGCGTGTTCATGGCTTTCTATATTGTTGATGCCTTGCCCATCCGGACCCCACTCCtt  >  1:225001/1‑251 (MQ=255)
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GCGAAAATTAAGGAGATGTTATGACACAGTCCAATCGGATCACTGCATCATCACCAGTGATCTAAATACTTCACAGCCATTCTCGGTCGCAGGTATCAAGCTTTTCAGGATTCCACAAATATTGTAACCCTGACGTCTAATTTGGTTATATAACATACTGCCCCAACTAACGTAAGGTAGGCACAGCCTTAATCAAGGTTTTGGTTTTCAAATAGTTCAGAGCTGAGACCGCCAACC‑‑‑‑‑‑‑‑G‑C‑‑‑‑‑‑‑ACTATGGCA‑‑‑‑‑‑‑‑CTATGGCACTATGGCACTATGGCACTATGGCACTATGGCACCGCCAGCGATTGTAATCACACTTGATATTATAAAACACAGTTGCACGCATTATTTCCTGGTTGGTAGGGTCATATCTCGATGCTCTTTGAGCAATGTCAACATCGCGTGTTCATGGCTTTCTATATTGTTGATGCCTTGCCCATCCGGACCCCACTCCTT  >  NC_000913/2765175‑2765623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑