New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2290113	NA (NA)	6 (0.240)	6/484	1.9	18.8%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	4215396 =	26 (1.020)					intergenic (+187/‑82)	metA/aceB	homoserine O‑succinyltransferase/malate synthase A
Rejected: Frequency below/above cutoff threshold.

CTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCA  >  NC_000913/4215149‑4215638                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   cTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCtt                                                                                                                                                                                                                                                   >  1:130534/1‑249 (MQ=255)       cGTCTACCAAATCACGCCATAAGATCTACTGCACCTGAATCCAACGCTGAATTAATCTTCTGAGATAATCGATCGTTAAGCGCTTCAGCAACTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTACGACACGCCCCTCAGCAGGTGACTACAATTTATTAATATTGTTTTCCTCACGTTTTCAAGTATTTTTCATTATATTTGCATTTATGTTTGATTTTGGATTTTAAATGAGTATTCTTAGTtgttc                                                                                                                                                                                                                                           >  2:124072/1‑249 (MQ=255)            aCCAGAACACGCCTTACGATCGCCGGTACATGAATCCAACGCTGGATTTATCTTCTGTGAAAGTCGATCTTTAAGCGATTCAGCACCTGACCTCAGGCCCCTTCGGGTCCCTTATGTATTGCCGAAACGTGCCTCAGCAGGTTAATAAATTTTATTCAGATTGTTATCAATAATTTACCAATTATTTTTAATTAAAATGGAAATTGTCTCTGATATTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAAc                                                                                                                                                                                                                                      <  2:100477/251‑1 (MQ=255)                                  aCGGCACAAGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGAGCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAAGTTTATTCATATTGTTATCAACAAGTTATCAAGTATGTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTc                                                                                                                                                                                                                <  2:130534/251‑1 (MQ=255)                                    ggCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGt                                                                                                                                                                                                              <  1:143992/251‑1 (MQ=255)                                                        cgatgacttccaccatgttttgcaatggcagaatctgctccatgcgggacgagaaaatctcttttatggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCg                                                                                                                                                                                          <  1:88956‑M2/59‑1 (MQ=255)                                                                   accatgttttgccatggcagaatctgctccatgcgggacaagaaaatctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTTATGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGtt                                                                                                                                                                                              >  2:93199‑M2/182‑236 (MQ=255)                                                                   accatgttttgccatggcagaatctgctccatgcgggacaagaaaatctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTTATGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGtt                                                                                                                                                                                              <  1:93199‑M2/55‑1 (MQ=255)                                                                           tCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGAATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTg                                                                                                                                                                       >  1:184397/1‑251 (MQ=255)                                                                                  ttAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATg                                                                                                                                                                >  1:275712/1‑251 (MQ=255)                                                                                       cGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTGTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTcaca                                                                                                                                                                                                         <  1:66190/205‑1 (MQ=255)                                                                                       cGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAAAGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTGTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTcacc                                                                                                                                                                                                         >  2:66190/1‑204 (MQ=255)                                                                                                    tACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGcaacaaa                                                                                                                                               >  1:83138/1‑249 (MQ=255)                                                                                                               ccTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACACGTTATCAAGTATTTTTAATTACAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACt                                                                                                                                   >  2:194808/1‑251 (MQ=255)                                                                                                                cTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTg                                                                                                                                  >  1:138337/1‑251 (MQ=255)                                                                                                                       ttctggtctgacggcgcttactgctgaattcactgtcggcgaaggaaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTc                                                                                                                            >  2:147142‑M2/130‑250 (MQ=255)                                                                                                                              tttATTTCCGAAACTCACCTCATCAGGTGAATTAATTGTATTCATATTGTTATCAACAAGTTAGCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTATACTGAGTTTTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAATGGTGAAGTTTTCGGATCCATTACGAGGGGCTTCACGATGTCTGAACAGGATACAACAACCGATGAACTGGCTTTTACaatgc                                                                                                                     <  2:45098/250‑4 (MQ=255)                                                                                                                                   ttCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTAt                                                                                                                <  1:194808/250‑1 (MQ=255)                                                                                                                                        aaaCGTACCGCTGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAAGTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGGGCTGAACGAAAAGAGAACAACGAGCCTTCGTACACAGTGGGGAAGTTGTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACATCAACCGAGGAACTGGTTCTCACAAGGCCGTATGGTGAg                                                                                                          <  2:45893/251‑1 (MQ=255)                                                                                                                                                  attcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGc                                                                                                >  1:39641‑M2/103‑251 (MQ=255)                                                                                                                                                          gAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTAGTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCtt                                                                                        <  1:198202/251‑1 (MQ=255)                                                                                                                                                                       cgatcttGTTATCAACAAGTTATCAAGTATTTGTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAAGGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATTAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAATCAAATTCTTACTGCTGaatcg                                                                            <  2:40207/245‑4 (MQ=255)                                                                                                                                                                             tGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGCTTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCATCGTTCACAGTGGGGCAGTTTTCGAATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGCTGAACTGGATTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGACGAAGCg                                                                            >  1:40207/1‑244 (MQ=255)                                                                                                                                                                                                         aattaaAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTa                                          <  1:26914/250‑1 (MQ=255)                                                                                                                                                                                                             aaaaTGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGcc                                      >  1:8085/1‑250 (MQ=255)                                                                                                                                                                                                                   aacatgatctcatatcagggacttgttcgcaccttccTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTGTTCGGAT‑CATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCa                              <  1:23191‑M2/214‑1 (MQ=255)                                                                                                                                                                                                                      aTTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGACCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAAt                            >  1:3456/1‑251 (MQ=255)                                                                                                                                                                                                                        tGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACACCGCAATa                           >  2:27427/1‑250 (MQ=255)                                                                                                                                                                                                                         gTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATaa                          >  2:256353/1‑250 (MQ=255)                                                                                                                                                                                                                           ttttGATTTTGCATTTTACATGAGTAGTCTTAGTTGTGATGAACGAATAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGTTGAACGATGATTGAAAAGGCAACAACAACCGCTGAACTGGCTTTCACAAGGCCGTATGGCAAGCAGGAGAAGAAAATTCTTCCTGCCGAAGCGGTAGCATTTATGACTGAGCTGATGAAGCATTATACGCAACAACGCAATaacct                       >  2:225676/1‑248 (MQ=255)                                                                                                                                                                                                                                             aaTGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGTTCCATGACGAGGAGCTGCACGATGACTGAACAGGCAAAAACAACCGATGAACTGGCTTTCACAAGGCCGGATGGCGAGGAGGAGAAGCAAATTCTTACTGCCGCAGGGGTAGAAGTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAAAAAACTTCTGGCAGCGCGCATTc      >  2:161973/1‑250 (MQ=255)                                                                                                                                                                                                                                                gAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTcagca  <  1:263519/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCA  >  NC_000913/4215149‑4215638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.