New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1676530	36 (1.410)	3 (0.120)	3/478	2.7	8.5%	coding (1374/1533 nt)	pntA	pyridine nucleotide transhydrogenase subunit alpha
	?	NC_000913	1677140 =	29 (1.150)					coding (764/1533 nt)	pntA	pyridine nucleotide transhydrogenase subunit alpha
Rejected: Frequency below/above cutoff threshold.

GTTTCAGCAGATTAACGAGGTTTGTGCCGTAAAGCTGTGAGGATTGCGTCGGCAGACGGCCCGGAAGATCGGTATAACCAATCAC‑TTTGACACCATTTTCCGTAGTGAAGATTTCACCCGGCACGGTGTATTCACAGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCGAATGCACGCACAATCGCGCCGAGACTGTTTGCTGCGCCAATGGCGGCCAGACCTGCAAC  >  NC_000913/1676895‑1677381                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gTTTCAGCAGATTAACGAGGTTTGTGCCGTAAAGCTGTGAGGATTGCGTCGTCAGACGGCCCGGAAGATCGGTATAACCAATCAC‑TTTGACACCATTTTCCGTAGTGAAGATTTCACCCGGCACGGTGTATTCACATTTGCCGACGTTTTGGGCTGCCAGGTCGACAATCACACTTCCCGCCTTCATGGAGTCAACCATTTCCCGTGTACTTAGCTCAGGCGCTGTTTTGCCTGGAATACGCGCGggggtg                                                                                                                                                                                                                                              >  2:218517/1‑251 (MQ=255)               aCGAGGTTTGTGCCGTAAAGCTGTGAGGATTGCGTCGGCAGACGGCCCGGAAGATCGGTATAACCAATCAC‑TTTGACACCATTTTCCGTAGTGAAGATTTCACCCGGCACGGTGTATTCACAGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGAc                                                                                                                                                                                                                                <  1:218517/251‑1 (MQ=255)                                              gCGTCGGCAGACGGCCCGGAAGATCGGTATAACCAATCAC‑TTTGACACCATTTTCCGTAGTGAAGATTTCACCCGGCACGGTGTATTCACAGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCAtt                                                                                                                                                                                                 <  1:37117/251‑1 (MQ=255)                                                     ccatgtgaccGGAAGATCGGTATAACCAAT‑ACATTTACCACCATATTCCGTGGTGTAGATATCACCCGGCTCGGTGTATTCACTGTTGCCGCCGTTTTGGTCAGCAAGGTCGACAATCACACTGCCCGCCTTCATGGAGACAACCATTTCACGGGAAGTTAGCTACGGCGCTGGTTTGCCTGGAATAAGCGAGGTGGTGACAATGATAGCGACCTCGTTTGCCTGGGCGGCAAAGAGTTCCATTTcctcct                                                                                                                                                                                          <  2:67007/243‑5 (MQ=255)                                                                       ggTATAACCAATCAC‑TTTGACACCATTTTCCGTAGTGAAGATTTCACCCGGCACGGTGTATTCACAGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACa                                                                                                                                                                        <  1:125513/251‑1 (MQ=255)                                                                          ataACCAATCAC‑TTTGACACCATTTTCCGTAGTGAAGATTTCACCCGGCACGGTGTATTCACAGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCa                                                                                                                                                                     <  1:258050/251‑1 (MQ=255)                                                                             aCCAATCAC‑TTTGACACCATTTTCCGTAGTGAAGATTTCACCCGGCACGGTGTATTCACAGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACtt                                                                                                                                                                  <  1:266625/251‑1 (MQ=255)                                                                                    aC‑TTTGACACCATTTTCCGTAGTGAAGATTTCACCCGGCACGGTGTATTCACAGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCAt                                                                                                                                                            >  1:208008/1‑250 (MQ=255)                                                                                                          ggaaaGATTTCACCCGGCACGGTGCTTGCTCATGTGCCCCCGTTTTGGGCTGCCAGTGCGACAACTACACGGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCtt                                                                                                                                       <  2:278372/247‑1 (MQ=255)                                                                                                                     ccccGGAACGGTGTATCCTCAGTTGCCGCCGTTTTGGGCAGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTaaaac                                                                                                                           <  2:24708/250‑2 (MQ=255)                                                                                                                            aCGGTGTATTCACAGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACAATGCCCGCCTTCATGGAGTCAACAATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGAGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTc                                                                                                                    >  1:35614/1‑251 (MQ=255)                                                                                                                                gttATTCACAGTTTCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAAACATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAgg                                                                                                                <  1:46800/249‑1 (MQ=255)                                                                                                                                      cacaGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTcc                                                                                                          >  2:138505/1‑251 (MQ=255)                                                                                                                                      accacttgtcgacgtgttGGTCTGCCAGGCCGTCAATCTCCCTGCCCGCCTTCATGGAGTCAACCATTTCACGTGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGGGGTGACAATGATATCGTCCTCTTTTGCCTGGGTGGCAAAGAGTCCCATTTCCGCTTTGATGAACTCGTCCGACATCACTTTTGCATAGCCATCGCCGTTTTCAGCTTCCTCTCTAAAATCCAGCTCGATGAATTcc                                                                                                          <  2:170421/235‑1 (MQ=255)                                                                                                                                                attaatgctggctataagcaccgcgataaaactaaggaagctaacccagccgccctggccaatctgcaacagtgctccgacaacaataatccctgaaatcgcgGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTtgat                                                                                                                                                                                      >  2:74022‑M2/104‑165 (MQ=255)                                                                                                                                                attaatgctggctataagcaccgcgataaaactaaggaagctaacccagccgccctggccaatctgcaacagtgctccgacaacaataatccctgaaatcgcgGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTtgat                                                                                                                                                                                      <  1:74022‑M2/62‑1 (MQ=255)                                                                                                                                                            tGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGtt                                                                                    >  1:173814/1‑251 (MQ=255)                                                                                                                                                              ccAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCt                                                                                  >  1:281014/1‑251 (MQ=255)                                                                                                                                                                    cGACAATCTCACGGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCAc                                                                             <  2:243532/250‑1 (MQ=255)                                                                                                                                                                    cGACAATCACCCTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTACGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACt                                                                            <  1:108085/251‑1 (MQ=255)                                                                                                                                                                         aTCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGAGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGAGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGACCTTGTTCTTTCACTTCcgg                                                                       >  1:30001/1‑251 (MQ=255)                                                                                                                                                                                 gCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTACGGCGCTGATGTGCCTGGAATAAGTGCGGGGGTGACAAAGGTATCAACCTCTTTTGGCTGGGCGGCAAAGAGTTCAATTTCAGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCAACGCCGATGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAGTTCAGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGCCGGgtgg                                                               >  1:245694/1‑250 (MQ=255)                                                                                                                                                                                   ccGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCg                                                             >  2:93302/1‑251 (MQ=255)                                                                                                                                                                                             ggAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCGAATgcacgc                                                    >  1:9316/1‑250 (MQ=255)                                                                                                                                                                                                 tCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGGGTCGAATGCACGCACAAt                                               >  1:240280/1‑251 (MQ=255)                                                                                                                                                                                                                   aTTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCGAATGCACGCACAGTCGCGCCGAGACTGTTtgc                             >  1:164744/1‑251 (MQ=255)                                                                                                                                                                                                                    ttAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAAAGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCACATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCGAATGCACGCACAATAGCGCCGCGACTGTTtgct                            >  2:108883/1‑251 (MQ=255)                                                                                                                                                                                                                      aGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCAACTGACCGCACAATCCGGCCGAGATTGTTttctgc                          >  2:52549/1‑251 (MQ=255)                                                                                                                                                                                                                        cttaggCGCTGGTTTGCCTGGAATAAGCGCCGGGGTGACAATGATATCGAACTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTTAACTTGTTCTTTCACTTCCGGGCGGGAGTCGCATGCACGCACAATCGCGCCGTGCCTGTTTGCTgcgc                        >  2:280518/5‑251 (MQ=255)                                                                                                                                                                                                                                 tGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCGAATGCACGCACAATCGCGCCGAGACTGTTTGCTGCGCCAATggcgg               >  1:132077/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCGAATGCACGCACAATCGCGCCGAGACTGTTTGCTGCGCCAATggcggc              >  1:175931/1‑251 (MQ=255)                                                                                                                                                                                                                                              ataatcgcgGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCGAATGCACGCACAATCGCGCCGAGACTGTTTGCTGCGCCAATGGCGGCCAGACCTGCAac  <  2:132077‑M2/242‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GTTTCAGCAGATTAACGAGGTTTGTGCCGTAAAGCTGTGAGGATTGCGTCGGCAGACGGCCCGGAAGATCGGTATAACCAATCAC‑TTTGACACCATTTTCCGTAGTGAAGATTTCACCCGGCACGGTGTATTCACAGTTGCCGCCGTTTTGGGCTGCCAGGTCGACAATCACACTGCCCGCCTTCATGGAGTCAACCATTTCACGGGTAATTAGCTTCGGCGCTGGTTTGCCTGGAATAAGCGCGGTGGTGACAATGATATCGACCTCTTTTGCCTGGGCGGCAAAGAGTTCCATTTCCGCTTTGATGAACGCGTCCGACATCACTTTGGCATAGCCATCGCCGCTGCCAGCTTCCTCTTTAAAATCCAGCTCGAGGAATTCCGCGCCCATACTTTGAACTTGTTCTTTCACTTCCGGGCGGGTGTCGAATGCACGCACAATCGCGCCGAGACTGTTTGCTGCGCCAATGGCGGCCAGACCTGCAAC  >  NC_000913/1676895‑1677381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.