Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,405,840	+T	coding (802/939 nt)	lrhA ←	DNA‑binding transcriptional dual regulator LrhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,405,840	1	.	T	100.0%	15.8 / NA	7	Y268N (TAT→AAT)	lrhA	DNA‑binding transcriptional dual regulator LrhA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (4/3);  total (4/3)

AGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATA‑GCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGG‑GCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGG  >  NC_000913/2405627‑2406053                                                                                                                                                                                                                       |                                                                                                                                                                                                                       aGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGAAAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATATGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTaa                                                                                                                                                                                    <  1:289060/251‑1 (MQ=255)                                                 gTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATATGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGGCCTCTAATTCACACAACCGACGCAGGTCCGG‑GCTCATCATCTCAAc                                                                                                                                   >  2:202461/1‑251 (MQ=255)                                                             atctatcGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATATGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCCTCTAATCCACTCAGCACACGCAGGGCCGG‑GGTCAACATCTCAACCCGCCTTGCCGt                                                                                                                       >  1:64293/1‑251 (MQ=255)                                                                                                                                         gTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATATGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCAATCAGCACACGCAGGTCCGG‑GCTCATCATCTCAAACGGCCTTTCCGTCCCGCCACGACCGGCTTTCACTGCCGCACGAACCGCCGGAAACGTCGGGGGGGAATAAGCCAGCCGCCATGGAat                                            >  1:178190/1‑250 (MQ=255)                                                                                                                                                cACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATATGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCCGCACACGCAGGGCCGG‑GCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCCAGAAAGGCCGGAAGAGTCGAGGCGACATAAACCCGGCGACATGGAATATCTGCtt                                    >  1:1443/1‑251 (MQ=255)                                                                                                                                                                                 aTCACCTGGGCCAGCTCATTATTACTGGACGGATCATATGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGG‑GCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACg   <  1:208158/251‑1 (MQ=255)                                                                                                                                                                                   cACCTGGGCCAGTGTATAATTACTGGACGGAGCGTATGCACAGCAAACATCCGGTATCAGGTAGGGGATTTAAGCCATCTACTACACGCAGCACACTCAGGTCCGGTACT‑TTCATCTCACCCTGCATTGCAGTCACGCCAAGATCGGTTTTCACTGCAGCACGAACGGCCGGAAGCGTCTAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGGCGACAGCACCATAGCGCGAAACgg  <  2:286766/250‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                       AGCCGCTGGGGTTTAAAACACCCCCAGCGGCTCGTTTTTTACACTATTGTCTCAGGAATTATCTATCGTCCGTCGATTACTCGATATCCCTTTCAATCAACAATGAATCATCCCCTTCCGGAGCAGACATTGGGCTGTATTGCCACGGATTATGGTAGCTCTCCATCGCCTGATAGATCACCTGGGCCAGCTCATTATTACTGGACGGATCATA‑GCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGG‑GCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGG  >  NC_000913/2405627‑2406053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑