Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,883	1	.	C	92.9%	78.5 / 1.0	28	coding (667/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (18/8);  total (19/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-01

CGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCT  >  NC_000913/3815636‑3816125                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    cgcgCCCGGTATGTAAGAGCCCGGCTTTGAAGAAATAGTGGCGTTTGCGCCCGGATGCCAGAGTAAACTCGCCAAACGTTAACACCTGCGTGCTAAGCGCAAATGCAATAAACTGGCGCGGATATTGTTTCATGCCTTCGCTCCACATCTGACATTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCGTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTccccctc                                                                                                                                                                                                                                                   <  2:135497/251‑4 (MQ=255)                    cccGGCGATGAAGAAATAGGGGCTTTTACGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCcaaca                                                                                                                                                                                                                                    >  2:152669/1‑247 (MQ=255)                                  aaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                                                                                                                                                                                                  >  2:254698/1‑251 (MQ=255)                                                                    cTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc                                                                                                                                                                                >  1:129910/1‑251 (MQ=255)                                                                                            gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                                                                                                                                        >  1:228704/1‑251 (MQ=255)                                                                                                          cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTCCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTACCCTGCACTTCAATGATGCGCCCGTCTTCGGTCCTCACTCCGTTc                                                                                                                                           >  2:33720/1‑250 (MQ=255)                                                                                                            aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTAGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATg                                                                                                                                        >  1:34413/1‑251 (MQ=255)                                                                                                                aCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCgg                                                                                                                                    <  1:294733/251‑1 (MQ=255)                                                                                                                     cgcTGATATGGTTTCATGCCTTCGCTACTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCATGCACTTCAATGTAGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTctct                                                                                                                               >  1:201045/1‑251 (MQ=255)                                                                                                                                  tCATGCCTTCGCTCCACATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTTCCAGCGCCGCCTTCTGCGTCGCTGCAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCATAGTCt                                                                                                                   <  1:207644/250‑1 (MQ=255)                                                                                                                                   cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTc                                                                                                                 >  1:239711/1‑251 (MQ=255)                                                                                                                                     tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGACATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCa                                                                                                                >  1:99391/1‑250 (MQ=255)                                                                                                                                                       tACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTcc                                                                                                       >  1:154653/1‑241 (MQ=255)                                                                                                                                                       tACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGAGGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTcc                                                                                                       <  2:154653/241‑1 (MQ=255)                                                                                                                                                                acaaaCAAAAAAAAGGCGACTCACCCATCTACTTTAAAATCAGTTTGCCCGCGCCGCCTTCTGCGTCGATACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCCCTTCAAGGATGCGCCCGTCTGCGGTCATCACTACGTTCAGGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CTCAAACCGCtt                                                                                      <  2:194160/244‑1 (MQ=255)                                                                                                                                                                     cAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCCCTACGTTCATTTCTGTCTCTGCGGCAGAGGCTTCAACGTATTCCAGAT‑CGCAAACCGCTCCGCCGTTc                                                                              >  2:38075/1‑251 (MQ=255)                                                                                                                                                                        aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑AGCAAACCGCTTCGCCGTTcac                                                                            >  2:252914/1‑250 (MQ=255)                                                                                                                                                                         aaaaaGGCGACCCATCAGTCGCTTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCTCCTTCTGCCGTCCCCTGCACTTCAATGTTGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACaa                                                                          <  1:21960/251‑1 (MQ=255)                                                                                                                                                                                  aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑AGCAAACCGCTTCGCCGTTCACAATTCCGACAg                                                                 <  1:252914/251‑1 (MQ=255)                                                                                                                                                                                         aGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGc                                                          >  1:212328/1‑251 (MQ=255)                                                                                                                                                                                                 gaaaaaTCAGTTTGCCAGAGCCGCCTTCTGCGTCGCTACAATGAATTCAATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGACCGGCTCGCCTTCTGCCGACCCCTGCACTTCAATGATGCGACCGACTTCGGTCCTCACTACGTTCATACCGCCATCTGCGGCAGATACTTCAACGAATTCCA‑ATACGCCAACCACTTCGCCGTTCACAATTCCGCCAGAAACTGCGGcaagcc                                                  >  2:185615/2‑246 (MQ=255)                                                                                                                                                                                                      aTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCTATTCCCCCTAGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCCTGTCGGTCTCTGCGGCAGAGTCTTCAACGCATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCAACCATCCCt                                             >  1:142436/1‑251 (MQ=255)                                                                                                                                                                                                       tCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGACCGGCTCGCCTTCTGCCGTCCCCTGCACTTCCATGATGCGCCCGTCTTCTGTCATCACTACGTTCCTGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGCT‑CGCTAACCGCTTCACCGTTCACAATTCCGACAGAAACTGccgatacacaccctg                                            >  2:69983/1‑237 (MQ=255)                                                                                                                                                                                                                cAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGAt                                   <  1:87086/251‑1 (MQ=255)                                                                                                                                                                                                                           tCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTAATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGc                        >  1:277107/1‑251 (MQ=255)                                                                                                                                                                                                                              gCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCt                       <  1:154291/249‑1 (MQ=255)                                                                                                                                                                                                                                gtagCTACAATGGATTCGCTTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGCGAACGGCACGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTAGGACATCACTACGTTCATGTCGGCCTCCGCGGACGAGTCTTCAACGTATTCCAGAT‑CGCCAACCGCTTTGCCGTTCCTCATACCGTCAATAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGATTgac                   >  2:106465/4‑249 (MQ=255)                                                                                                                                                                                                                                     tACAATGGATTCGATTCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCTGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTc             >  1:53229/1‑251 (MQ=255)                                                                                                                                                                                                                                                cgattcCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAGGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCTGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCt  <  2:53229/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    CGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAT‑CGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCT  >  NC_000913/3815636‑3816125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑